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Synthesis of Hybrid DNA-Protein Nanostructures

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While DNA and protein nanotechnologies are promising avenues for nanotechnology on their own, merging the two could create more diverse and functional structures. In order to create hybrid structures, the protein will have to undergo site-specific modification, such as the incorporation of an unnatural amino, p-azidophenylalanine (AzF), via Shultz amber…

While DNA and protein nanotechnologies are promising avenues for nanotechnology on their own, merging the two could create more diverse and functional structures. In order to create hybrid structures, the protein will have to undergo site-specific modification, such as the incorporation of an unnatural amino, p-azidophenylalanine (AzF), via Shultz amber codon suppression method, which can then participate in click chemistry with modified DNA. These newly synthesized structures will then be able to self-assemble into higher order structures. Thus far, a surface exposed residue on the aldolase protein has been mutated into an amber stop codon. The next steps are to express the protein with the unnatural amino acid, allow it to participate in click chemistry, and visualize the hybrid structure. If the structure is correct, it will be able to self-assemble.

ContributorsAziz, Ann-Marie (Author) / Stephanopoulos, Nicholas (Thesis director) / Mills, Jeremy (Committee member) / School of Social Transformation (Contributor) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)

Created2017-05

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Synthesis of Hybrid DNA-Protein Nanostructures

Partita no. 1 in B minor, BWV 1002. Double

Sonata no. 1 in G minor, BWV 1001. Presto

Brandenburg concerto, no. 4 BWV 1049. Andante

Brandenburg concerto, no. 4 BWV 1049. Presto

Fugue

Cello suite no. 2 in D minor, BWV 1008. Prelude

Cello suite no. 2 in D minor, BWV 1008. Allemande

Cello suite no. 2 in D minor, BWV 1008. Courante

Cello suite no. 2 in D minor, BWV 1008. Sarabande