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ABSTRACT Enzyme-Induced Carbonate Precipitation (EICP) using a plant-derived form of the urease enzyme to induce the precipitation of calcium carbonate (CaCO3) shows promise as a method of stabilizing soil for the mitigation of fugitive dust. Fugitive dust is a significant problem in Arizona, particularly in Maricopa County. Maricopa County is an EPA air quality non-attainment zone, due primarily to fugitive dust, which presents a significant health risk to local residents. Conventional methods for fugitive dust control, including the application of water, are either ineffective in arid climates, very expensive, or limited to short term stabilization. Due to these limitations, engineers are searching for new and more effective ways to stabilize the soil and reduce wind erosion. EICP employs urea hydrolysis, a process in which carbonate precipitation is catalyzed by the urease enzyme, a widely occurring protein found in many plants and microorganisms. Wind tunnel experiments were conducted in the ASU/NASA Planetary Wind Tunnel to evaluate the use of EICP as a means to stabilize soil against fugitive dust emission. Three different soils were tested, including a native Arizona silty-sand, a uniform fine to medium grained silica sand, and mine tailings from a mine in southern Arizona. The test soil was loosely placed in specimen container and the surface was sprayed with an aqueous solution containing urea, calcium chloride, and urease enzyme. After a short period of time to allow for CaCO3 precipitation, the specimens were tested in the wind tunnel. The completed tests show that EICP can increase the detachment velocity compared to bare or wetted soil and thus holds promise as a means of mitigating fugitive dust emissions.
ContributorsKnorr, Brian (Author) / Kavazanjian, Edward (Thesis advisor) / Houston, Sandra (Committee member) / Zapata, Claudia (Committee member) / Arizona State University (Publisher)
Created2014