Matching Items (3)

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Biology-based matched signal processing and physics-based modeling for improved detection

Description

Peptide microarrays have been used in molecular biology to profile immune responses and develop diagnostic tools. When the microarrays are printed with random peptide sequences, they can be used

Peptide microarrays have been used in molecular biology to profile immune responses and develop diagnostic tools. When the microarrays are printed with random peptide sequences, they can be used to identify antigen antibody binding patterns or immunosignatures. In this thesis, an advanced signal processing method is proposed to estimate epitope antigen subsequences as well as identify mimotope antigen subsequences that mimic the structure of epitopes from random-sequence peptide microarrays. The method first maps peptide sequences to linear expansions of highly-localized one-dimensional (1-D) time-varying signals and uses a time-frequency processing technique to detect recurring patterns in subsequences. This technique is matched to the aforementioned mapping scheme, and it allows for an inherent analysis on how substitutions in the subsequences can affect antibody binding strength. The performance of the proposed method is demonstrated by estimating epitopes and identifying potential mimotopes for eight monoclonal antibody samples.

The proposed mapping is generalized to express information on a protein's sequence location, structure and function onto a highly localized three-dimensional (3-D) Gaussian waveform. In particular, as analysis of protein homology has shown that incorporating different kinds of information into an alignment process can yield more robust alignment results, a pairwise protein structure alignment method is proposed based on a joint similarity measure of multiple mapped protein attributes. The 3-D mapping allocates protein properties into distinct regions in the time-frequency plane in order to simplify the alignment process by including all relevant information into a single, highly customizable waveform. Simulations demonstrate the improved performance of the joint alignment approach to infer relationships between proteins, and they provide information on mutations that cause changes to both the sequence and structure of a protein.

In addition to the biology-based signal processing methods, a statistical method is considered that uses a physics-based model to improve processing performance. In particular, an externally developed physics-based model for sea clutter is examined when detecting a low radar cross-section target in heavy sea clutter. This novel model includes a process that generates random dynamic sea clutter based on the governing physics of water gravity and capillary waves and a finite-difference time-domain electromagnetics simulation process based on Maxwell's equations propagating the radar signal. A subspace clutter suppression detector is applied to remove dominant clutter eigenmodes, and its improved performance over matched filtering is demonstrated using simulations.

Contributors

Agent

Created

Date Created
  • 2014

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Separation of Agile Waveform Time-Frequency Signatures from Coexisting Multimodal Systems

Description

As the demand for wireless systems increases exponentially, it has become necessary

for different wireless modalities, like radar and communication systems, to share the

available bandwidth. One approach to realize coexistence successfully

As the demand for wireless systems increases exponentially, it has become necessary

for different wireless modalities, like radar and communication systems, to share the

available bandwidth. One approach to realize coexistence successfully is for each

system to adopt a transmit waveform with a unique nonlinear time-varying phase

function. At the receiver of the system of interest, the waveform received for process-

ing may still suffer from low signal-to-interference-plus-noise ratio (SINR) due to the

presence of the waveforms that are matched to the other coexisting systems. This

thesis uses a time-frequency based approach to increase the SINR of a system by estimating the unique nonlinear instantaneous frequency (IF) of the waveform matched

to the system. Specifically, the IF is estimated using the synchrosqueezing transform,

a highly localized time-frequency representation that also enables reconstruction of

individual waveform components. As the IF estimate is biased, modified versions of

the transform are investigated to obtain estimators that are both unbiased and also

matched to the unique nonlinear phase function of a given waveform. Simulations

using transmit waveforms of coexisting wireless systems are provided to demonstrate

the performance of the proposed approach using both biased and unbiased IF estimators.

Contributors

Agent

Created

Date Created
  • 2018

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Adaptive methods within a sequential Bayesian approach for structural health monitoring

Description

Structural integrity is an important characteristic of performance for critical components used in applications such as aeronautics, materials, construction and transportation. When appraising the structural integrity of these components, evaluation

Structural integrity is an important characteristic of performance for critical components used in applications such as aeronautics, materials, construction and transportation. When appraising the structural integrity of these components, evaluation methods must be accurate. In addition to possessing capability to perform damage detection, the ability to monitor the level of damage over time can provide extremely useful information in assessing the operational worthiness of a structure and in determining whether the structure should be repaired or removed from service. In this work, a sequential Bayesian approach with active sensing is employed for monitoring crack growth within fatigue-loaded materials. The monitoring approach is based on predicting crack damage state dynamics and modeling crack length observations. Since fatigue loading of a structural component can change while in service, an interacting multiple model technique is employed to estimate probabilities of different loading modes and incorporate this information in the crack length estimation problem. For the observation model, features are obtained from regions of high signal energy in the time-frequency plane and modeled for each crack length damage condition. Although this observation model approach exhibits high classification accuracy, the resolution characteristics can change depending upon the extent of the damage. Therefore, several different transmission waveforms and receiver sensors are considered to create multiple modes for making observations of crack damage. Resolution characteristics of the different observation modes are assessed using a predicted mean squared error criterion and observations are obtained using the predicted, optimal observation modes based on these characteristics. Calculation of the predicted mean square error metric can be computationally intensive, especially if performed in real time, and an approximation method is proposed. With this approach, the real time computational burden is decreased significantly and the number of possible observation modes can be increased. Using sensor measurements from real experiments, the overall sequential Bayesian estimation approach, with the adaptive capability of varying the state dynamics and observation modes, is demonstrated for tracking crack damage.

Contributors

Agent

Created

Date Created
  • 2013