Forensic entomology is the use of insects in legal investigations, and relies heavily upon calculating the time of colonization (TOC) of insects on remains using temperature-dependent growth rates. If a body is exposed to temperatures that exceed an insect’s critical limit, TOC calculations could be severely affected. The determination of critical thermal limits of forensically-relevant insects is crucial, as their presence or absence could alter the overall postmortem interval (PMI) calculation. This study focuses on the larvae of Phormia regina (Meigen) (Diptera: Calliphoridae), a forensically relevant blow fly common across North America. Three populations were examined (Arizona, Colorado, and New Jersey), and five day old larvae were exposed to one of two temperatures, 39℃ or 45℃, for five hours. Across all colonies, the survival rate was lower at 45℃ than 39℃, in both larval and emerged adult stages. The Arizona colony experienced a harsher drop in survival rates at 45℃ than either the Colorado or New Jersey colonies. This research suggests that the range of 39℃ - 45℃ approaches the critical thermal limit for P. regina, but does not yet exhibit a near or complete failure of survivorship that a critical temperature would cause at this duration of time. However, there is opportunity for further studies to examine this critical temperature by investigating other temperatures within the 39℃ - 45℃ range and at longer durations of time in these temperatures.