Permafrost degradation is leading to rapid wetland formation in northern peatland ecosystems, altering the role of these ecosystems in the global carbon cycle. I reviewed the literature on the history of the MPP theory, including tracing its origins to The Second Law of Thermodynamics. To empirically test the MPP, I collected soils along a gradient of ecosystem development and: 1) quantified the rate of adenosine triphosphate (ATP) production--literally cellular energy--to test the MPP; 2) quantified greenhouse gas production (CO2, CH4, and N2O) and microbial genes that produce enzymes catalyzing greenhouse gas production, and; 3) sequenced the 16s rRNA gene from soil microbes to investigate microbial community composition across the chronosequence of wetland development. My results suggested that the MPP and other related theoretical constructs have strong potential to further inform our understanding of ecosystem development. Soil system power (ATP) decreased temporarily as the ecosystem reorganized after disturbance to rates of power production that approached pre-disturbance levels. Rates of CH4 and N2O production were higher at the newly formed bog and microbial genes involved with greenhouse gas production were strongly related to the amount of greenhouse gas produced. DNA sequencing results showed that across the chronosequence of development, the two relatively mature ecosystems--the peatland forest ecosystem prior to permafrost degradation and the oldest bog--were more similar to one another than to the intermediate, less mature bog. Collectively, my results suggest that ecosystem age, rather than ecosystem state, was a more important driver for ecosystem structure and function.
The overarching question of this dissertation is: how do hydrology, soil conditions, and plant patches affect patterns of denitrification in accidental urban wetlands? To answer this question, I took a three-pronged approach using a combination of field and greenhouse studies. First, I examined drivers of broad patterns of denitrification in accidental urban wetlands. Second, I used a field study to test if plant traits influence denitrification indirectly by modifying soil resources. Finally, I examined how species richness and interactions between species influence nitrate retention and patterns of denitrification using both a field study and greenhouse experiment.
Hydroperiod of accidental urban wetlands mediated patterns of denitrification in response to monsoon floods and plant patches. Specifically, ephemeral wetlands had patterns of denitrification that were largely unexplained by monsoon floods or plant patches, which are common drivers of patterns of denitrification in non-urban wetlands. Several plant traits including belowground biomass, above- and belowground tissue chemistry and rooting depth influenced denitrification indirectly by changing soil organic matter or soil nitrate. However, several other plant traits also had significant direct relationships with denitrification, (i.e. not through the hypothesized indirect relationships through soil organic matter or soil nitrate). This means these plant traits were affecting another aspect of soil conditions not included in the analysis, highlighting the need to improve our understanding of how plant traits influence denitrification. Finally, increasing species richness did not increase nitrate retention or denitrification, but rather individual species had the greatest effects on nitrate retention and denitrification.
Constructed treatment wetlands (CTW) have been a cost-efficient technological solution to treat different types of wastewater but may also be sources of emitters of methane (CH4) and nitrous oxide (N2O). Thus, my objective for this dissertation was to investigate CH4 and N2O fluxes via multiple pathways from the Tres Rios CTW located in Phoenix, AZ, USA. I measured gas fluxes from the CTW along a whole-system gradient (from inflow to outflow) and a within-marsh gradient (shoreline, middle, and open water sites). I found higher diffusive CH4 release in the summer compared to spring and winter seasons. Along the whole-system gradient, I found greater CH4 and N2O emission fluxes near the inflow compared to near the outflow. Within the vegetated marsh, I found greater CH4 emission fluxes at the vegetated marsh subsites compared to the open water. In contrast, N2O emissions were greater at the marsh-open water locations compared to interior marsh. To study the plant-mediated pathway, I constructed small gas chambers fitted to Typha spp. leaves. I found plant-mediated CH4 fluxes were greater near the outflow than near the inflow and that CH4 fluxes were higher from lower sections of plants compared to higher sections. Overall, Typha spp. emitted a mean annual daily flux rate of 358.23 mg CH4 m-2 d-1. Third, using a 30-day mesocosm experiment I studied the effects of three different drydown treatments (2, 7, 14 days) on the fluxes of CH4 and N2O from flooded CTW soils. I found that CH4 fluxes were not significantly affected by soil drydown events. Soils that were dry for 7 days shifted from being N2O sources to sinks upon inundation. As a result, the 7-day drydown soils were sinks while the 14-day drydown soils showed significant N2O release. My results emphasize the importance of studying ecological processes in CTWs to improve their design and management strategies so we can better mitigate their greenhouse gas emissions.
Nitrogen is an essential, often limiting, element for biological growth that can act as a pollutant if present in excess. Nitrogen is primarily transported by water from uplands to streams and eventually to recipient lakes, estuaries, and wetlands, but can be modulated by biological uptake and transformation along these flowpaths. As a result, nitrogen can accumulate in aquatic ecosystems if supply is high or if biological retention is low. Dryland and urban ecosystems offer interesting contrasts in water supply, which limits transport and biological activity in drylands, and nitrogen supply that increases with human activity. In my dissertation, I ask: What is the relative balance among nitrogen retention, removal, and transport processes in dryland watersheds, and what is the fate of exported nitrogen? My dissertation research demonstrates that water is a major control on where and when nitrogen is retained and removed versus exported to downstream ecosystems. I used a mass-balance model based on synoptic surveys to study seasonal and spatial patterns in nitrate loading to a dryland stream network. I found that irrigation diversions transport nitrate from agricultural areas to the stream network year-round, even during dry seasons, and are an important driver of nitrate loading. I further explored how seasonal precipitation influences flood nutrient export in an intermittent desert stream by coupling long-term data of flood-water chemistry with stream discharge and precipitation data. I found that higher precipitation prior to a flood fills water storage sites in the catchment, leading to larger floods. In addition, higher antecedent precipitation stimulates biological nitrogen retention in the uplands, leading to lower nitrogen concentration in floods. Finally, I evaluated the consequences of nitrogen export from watersheds on how urban wetlands attenuate nitrate through denitrification that permanently removes nitrogen, and dissimilatory nitrate reduction to ammonium (DNRA) that retains nitrogen in another biologically reactive form. I found that DNRA becomes proportionally more important with low nitrate concentration, thereby retaining nitrogen as ammonium. Collectively, my dissertation research addresses how dryland and urban ecosystems can be integrated into models of watershed nitrogen cycling.