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Combined AFM and Fluorescence Measurements for the Investigation of Nanophotonic Effects on Single Fluorophores

Description
In this project, we introduce a type of microscopy which produces correlated topography and fluorescence lifetime images with nanometer resolution. This technique combines atomic force microscopy (AFM) and time resolved confocal fluorescence microscopy to conduct biological and materials research. This method is used to investigate nanophotonic effects on single fluorophores,…

In this project, we introduce a type of microscopy which produces correlated topography and fluorescence lifetime images with nanometer resolution. This technique combines atomic force microscopy (AFM) and time resolved confocal fluorescence microscopy to conduct biological and materials research. This method is used to investigate nanophotonic effects on single fluorophores, including quantum dots and fluorescent molecules. For single fluorescent molecules, we investigate the effects of quenching of fluorescence with the probe of an atomic force microscope which is combined and synchronized with a confocal fluorescence lifetime microscope. For quantum dots, we investigate the correlation between the topographic and fluorescence data. With this method of combining an atomic force microscope with a confocal microscope, it is anticipated that there will be applications in nanomaterial characterization and life sciences; such as the determination of the structure of small molecular systems on surfaces, molecular interactions, as well as the structure and properties of fluorescent nanomaterials.
ContributorsWard, Alex Mark (Author) / Ros, Robert (Thesis director) / Shumway, John (Committee member) / Schulz, Olaf (Committee member) / Barrett, The Honors College (Contributor) / School of Mathematical and Statistical Sciences (Contributor) / Department of Physics (Contributor)
Created2013-05
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Examining Morphological Differences in UBC Subpopulations

Description

Unipolar brush cells (UBCs) are glutamatergic neurons in the cerebellar cortex. Their morphology includes a brush-like dendrite, soma, and axon with large ‘mossy fiber’ terminals. They are classified as being excited or inhibited by glutamate (ON and OFF). UBCs may contribute to auditory and vestibular circuits whose dysfunction may lead…

Unipolar brush cells (UBCs) are glutamatergic neurons in the cerebellar cortex. Their morphology includes a brush-like dendrite, soma, and axon with large ‘mossy fiber’ terminals. They are classified as being excited or inhibited by glutamate (ON and OFF). UBCs may contribute to auditory and vestibular circuits whose dysfunction may lead to tinnitus and ataxia, respectively. This study explores UBC physiology, connectivity in cerebellar circuits, and contributions to circuit dysfunction.
ContributorsAlgstam, Alexa Brynn (Author) / Balmer, Dr. Timothy (Thesis director) / Newbern, Dr. Jason (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor) / School of International Letters and Cultures (Contributor)
Created2022-05