Matching Items (3)
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- All Subjects: Soil Ecology
- Creators: Arnold, Susanne
- Creators: Garcia-Pichel, Ferran
- Resource Type: Text
Description
Human activity has increased loading of reactive nitrogen (N) in the environment, with important and often deleterious impacts on biodiversity, climate, and human health. Since the fate of N in the ecosystem is mainly controlled by microorganisms, understanding the factors that shape microbial communities becomes relevant and urgent. In arid land soils, these microbial communities and factors are not well understood. I aimed to study the role of N cycling microbes, such as the ammonia-oxidizing bacteria (AOB), the recently discovered ammonia-oxidizing archaea (AOA), and various fungal groups, in soils of arid lands. I also tested if niche differentiation among microbial populations is a driver of differential biogeochemical outcomes. I found that N cycling microbial communities in arid lands are structured by environmental factors to a stronger degree than what is generally observed in mesic systems. For example, in biological soil crusts, temperature selected for AOA in warmer deserts and for AOB in colder deserts. Land-use change also affects niche differentiation, with fungi being the major agents of N2O production in natural arid lands, whereas emissions could be attributed to bacteria in mesic urban lawns. By contrast, NO3- production in the native desert and managed soils was mainly controlled by autotrophic microbes (i.e., AOB and AOA) rather than by heterotrophic fungi. I could also determine that AOA surprisingly responded positively to inorganic N availability in both short (one month) and long-term (seven years) experimental manipulations in an arid land soil, while environmental N enrichment in other ecosystem types is known to favor AOB over AOA. This work improves our predictions of ecosystem response to anthropogenic N increase and shows that paradigms derived from mesic systems are not always applicable to arid lands. My dissertation also highlights the unique ecology of ammonia oxidizers and draws attention to the importance of N cycling in desert soils.
ContributorsMarusenko, Yevgeniy (Author) / Hall, Sharon J (Thesis advisor) / Garcia-Pichel, Ferran (Thesis advisor) / Mclain, Jean E (Committee member) / Schwartz, Egbert (Committee member) / Arizona State University (Publisher)
Created2013
Description
Mycorrhizal fungi form symbiotic relationships with plant roots, increasing nutrient and water availability to plants and improving soil stability. Mechanical disturbance of soil has been found to reduce mycorrhizal inoculum in soils, but findings have been inconsistent. To examine the impact of restoration practices on riparian mycorrhizal inoculum potential, soil samples were collected at the Tres Rios Ecosystem Restoration and Flood Control Project located at the confluence of the Salt, Gila, and Agua Fria rivers in central Arizona. The project involved the mechanical removal of invasive Tamarix spp.( tamarisk, salt cedar) and grading prior to revegetation. Soil samples were collected from three stages of restoration: pre-restoration, soil banks with chipped vegetation, and in areas that had been graded in preparation for revegetation. Bioassay plants were grown in the soil samples and roots analyzed for arbuscular mycorrhizal (AM) and ectomycorrhizal (EM) infection percentages. Vegetations measurements were also taken for woody vegetation at the site. The mean number of AM and EM fungal propagules did not differ between the three treatment area, but inoculum levels did differ between AM and EM fungi with AM fungal propagules detected at moderate levels and EM fungi at very low levels. These differences may have been related to availability of host plants since AM fungi form associations with a variety of desert riparian forbs and grasses and EM fungi only form associations with Populus spp. and Salix spp. which were present at the site but at low density and canopy cover. Prior studies have also found that EM fungi may be more affected by tamarisk invasions than AM fungi. Our results were similar to other restoration projects for AM fungi suggesting that it may not be necessary to add AM fungi to soil prior to planting native vegetation because of the moderate presence of AM fungi even in soils dominated by tamarisk and exposed to soil disturbance during the restoration process. In contrast when planting trees that form EM associations, it may be beneficial to augment soil with EM fungi collected from riparian areas or to pre-inoculate plants prior to planting.
ContributorsArnold, Susanne (Author) / Stutz, Jean (Thesis advisor) / Alford, Eddie (Committee member) / Green, Douglas (Committee member) / Arizona State University (Publisher)
Created2012
Description
Microarthropods play important roles in the decomposition process of the detrital food web, where they break down organic matter and return nutrients to the soil. However, only a small percentage of the belowground microarthropod population has been studied or even discovered, leading to a decrease in the knowledge of all of the processes carried out by these organisms and their importance to the soil. This is because microarthropod extraction methods are not 100% effective at collecting specimens. This study aimed to find an ideal quantitative procedure to better record the number of microarthropods existing in the soil and to determine if a seasonal variation exists that effects the success of extraction. Two extraction methods, including dynamic extraction and heptane flotation extraction, were compared across two seasons, a dry season (June) and a wet season (September). Average biomasses and average richness were calculated for four different functional groups, including Prostigmata, Mesostigmata, Cryptostigmata, and Collembola, across the two seasons, and statistical analysis was performed to determine if any differences that existed were statistically significant. Results indicate that the dynamic extraction method was significantly more effective for the collection of microarthropods during the wet season, and the heptane extraction method was significantly more effective during the dry season. In addition, the heptane procedure recovered samples of higher average richness than the dynamic method during both seasons. The heptane procedure works best for extraction during the dry season because it is able to collect organisms that entered into an ametabolic anhydrobiotic state to escape desiccation. These organisms form a protective lipid layer around their exoskeletons to retain water, and the non-polar exoskeletons display a chemical affinity to the heptane fluid, allowing for collection out of the soil and into the heptane layer. Despite these results, no one method is entirely superior to the other, and the most efficacious procedure depends on the researcher's aim of study.
ContributorsAntol, Rachel Lynn (Author) / Sabo, John L. (Thesis director) / Hall, Sharon (Committee member) / Wyant, Karl A. (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor)
Created2014-12