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- All Subjects: HMM
- Creators: Pollat, Scott
- Creators: Presse, Steve
Single molecule FRET experiments are important for studying processes that happen on the molecular scale. By using pulsed illumination and collecting single photons, it is possible to use information gained from the fluorescence lifetime of the chromophores in the FRET pair to gain more accurate estimates of the underlying FRET rate which is used to determine information about the distance between the chromophores of the FRET pair. In this paper, we outline a method that utilizes Bayesian inference to learn parameter values for a model informed by the physics of a immobilized single-molecule FRET experiment. This method is unique in that it combines a rigorous look at the photophysics of the FRET pair and a nonparametric treatment of the molecular conformational statespace, allowing the method to learn not just relevant photophysical rates (such as relaxation rates and FRET rates), but also the number of molecular conformational states.
Bdellovibrio bacteriovorus (BB) is a gram negative predatory bacteria that uses other gram negative bacteria to proliferate non-binarily. Due to the predatory nature of BB researchers have proposed to use it as a potential biocontrol agent against other gram negative bacteria. The in vivo effect of predatory bacteria on a living host lacks thorough investigation. This paper explores BB inside and outside of the C. elegans. BB acts internally by pre- infecting C. elegans with E. coli and then treating the worms with BB. After BB treatment worm survivavbility increased and morbidity decreased. Ex- ternally, BB modulated the environment around the nematode which reduced infection rates and increased nematode lifespan and survivability. Together, the internal and external results suggest BB has the capability to act as a living antibiotic acting topically and internally to reduce infection rates.