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Characterizing the activity of alcohol dehydrogenase from Lactobacillus brevis on primary and secondary alcohol biofuel precursors

Description
The R-specific alcohol dehydrogenase (RADH or LVIS_0347) from Lactobacillus brevis LB19 was found to possess activity on several short chain aldehydes and ketones. This broad substrate specificity was previously uncharacterized. To demonstrate its relevance to the biofuels industry as well as its broader utility for chiral reductions, a detailed characterization…

The R-specific alcohol dehydrogenase (RADH or LVIS_0347) from Lactobacillus brevis LB19 was found to possess activity on several short chain aldehydes and ketones. This broad substrate specificity was previously uncharacterized. To demonstrate its relevance to the biofuels industry as well as its broader utility for chiral reductions, a detailed characterization was performed to further investigate the activity and function of RADH.
ContributorsHalloum, Ibrahim (Co-author) / Pugh, Shawn (Co-author) / Nielsen, David R. (Thesis director) / Rege, Kaushal (Committee member) / Barrett, The Honors College (Contributor) / Chemical Engineering Program (Contributor)
Created2015-05

Process Design and Refinement for Bioproduction of Sustainable Butanol

Description
This document outlines the research work done by Shona Becwar in the process design and refinement for the production of sustainable butanol from Clostridium, along with the required background knowledge on the subject. The process that the microbiological organisms go through to produce butanol must be an oxygen free environment…

This document outlines the research work done by Shona Becwar in the process design and refinement for the production of sustainable butanol from Clostridium, along with the required background knowledge on the subject. The process that the microbiological organisms go through to produce butanol must be an oxygen free environment for up to 21 days with multiple perforations made into the environment in this period. There was not previously a cost effective method to do this, even in small scale. It was determined that using a butyl rubber septa would allow for the environment to be sustained during the growth process. The pervaporation process was losing butanol product at a rate of approximately 60%, changing the tubing from silicon to stainless steel allowed for a mere 7% loss during the separation process, greatly increasing the prospective of upscaling this process. These improvements to the sustainable butanol production process will allow for a more efficient, therefore more economically competitive product which can be used as a drop in equivalent to the current butanol market.
ContributorsBecwar, Shona Marie (Author) / Nielsen, David R. (Thesis director) / Staggs, Kyle (Committee member) / Chemical Engineering Program (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05