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Description
Four enzymes, ATF1, ATF2, ATF, and CAT, were screened to determine which would be most effective at catalyzing the formation of aromatic esters. The CAT enzyme successfully catalyzed the reaction to produce 2-phenethyl acetate using 20x more lysate to improve the probability of enzyme presence in the lysate. The CAT

Four enzymes, ATF1, ATF2, ATF, and CAT, were screened to determine which would be most effective at catalyzing the formation of aromatic esters. The CAT enzyme successfully catalyzed the reaction to produce 2-phenethyl acetate using 20x more lysate to improve the probability of enzyme presence in the lysate. The CAT enzyme was able to catalyze the reaction producing concentrations that increased by 62% every 12 hours. Enzymatic activity resulted in the production of 2.15 mg/L of 2-phenethyl acetate at 12 hours, 5.62 mg/L of 2-phenethyl acetate at 24 hours, and 15.12 mg/L of 2-phenethyl acetate at 48 hours.
ContributorsBrown, Kristen Ashley (Author) / Nielsen, David (Thesis director) / Thompson, Brian (Committee member) / Chemical Engineering Program (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
ContributorsDu, Pan (Performer) / ASU Library. Music Library (Publisher)
Created2018-04-23
ContributorsZhang, Aihua (Performer) / ASU Library. Music Library (Publisher)
Created2018-04-23
ContributorsSong, Haiyuan (Performer) / Jiang, Zhou (Performer) / ASU Library. Music Library (Publisher)
Created2018-04-22
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Description

Terpene cyclizations are one of the most complex reactions that occur in nature. Scientists have found that replicating this reaction in a lab setting has proved to be immensely challenging as result of the numerous intermediates that must be controlled through the cyclization process in the absence of an enzyme.

Terpene cyclizations are one of the most complex reactions that occur in nature. Scientists have found that replicating this reaction in a lab setting has proved to be immensely challenging as result of the numerous intermediates that must be controlled through the cyclization process in the absence of an enzyme. This study uses commercially available lipases to conduct reactions with geraniol-derived starting materials to identify conditions for performing a terpene cyclization effectively and efficiently. Through hypothesized screening of enzymes and reaction conditions, we have identified a protocol for the successful cyclization of limonene and other geranyl-derived products.

ContributorsGupta, Ritika (Author) / Biegasiewicz, Kyle (Thesis director) / Ackerman-Biegasiewicz, Laura (Committee member) / Heyden, Matthias (Committee member) / College of Health Solutions (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
ContributorsSoberano, Ramon Alfonso (Performer) / ASU Library. Music Library (Publisher)
Created2018-04-17
ContributorsRomani, Amanda (Performer) / Shraibman, Dani (Performer) / ASU Library. Music Library (Publisher)
Created2018-04-15
ContributorsShen, Mengyu (Performer) / ASU Library. Music Library (Publisher)
Created2018-04-16
ContributorsChen, Sicong (Performer) / Chen, Neilson (Performer)
Created2018-04-15
ContributorsZhou, Dana (Performer) / ASU Library. Music Library (Publisher)
Created2018-04-15