Matching Items (5)
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- All Subjects: Electrochemical Impedance Spectroscopy
- Creators: LaBelle, Jeffrey
- Creators: Janis, William Edward
- Resource Type: Text
Description
Bioscience High School, a small magnet high school located in Downtown Phoenix and a STEAM (Science, Technology, Engineering, Arts, Math) focused school, has been pushing to establish a computer science curriculum for all of their students from freshman to senior year. The school's Mision (Mission and Vision) is to: "..provide a rigorous, collaborative, and relevant academic program emphasizing an innovative, problem-based curriculum that develops literacy in the sciences, mathematics, and the arts, thus cultivating critical thinkers, creative problem-solvers, and compassionate citizens, who are able to thrive in our increasingly complex and technological communities." Computational thinking is an important part in developing a future problem solver Bioscience High School is looking to produce. Bioscience High School is unique in the fact that every student has a computer available for him or her to use. Therefore, it makes complete sense for the school to add computer science to their curriculum because one of the school's goals is to be able to utilize their resources to their full potential. However, the school's attempt at computer science integration falls short due to the lack of expertise amongst the math and science teachers. The lack of training and support has postponed the development of the program and they are desperately in need of someone with expertise in the field to help reboot the program. As a result, I've decided to create a course that is focused on teaching students the concepts of computational thinking and its application through Scratch and Arduino programming.
ContributorsLiu, Deming (Author) / Meuth, Ryan (Thesis director) / Nakamura, Mutsumi (Committee member) / Computer Science and Engineering Program (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
Electromyography (EMG) and Electroencephalography (EEG) are techniques used to detect electrical activity produced by the human body. EMG detects electrical activity in the skeletal muscles, while EEG detects electrical activity from the scalp. The purpose of this study is to capture different types of EMG and EEG signals and to determine if the signals can be distinguished between each other and processed into output signals to trigger events in prosthetics. Results from the study suggest that the PSD estimates can be used to compare signals that have significant differences such as the wrist, scalp, and fingers, but it cannot fully distinguish between signals that are closely related, such as two different fingers. The signals that were identified were able to be translated into the physical output simulated on the Arduino circuit.
ContributorsJanis, William Edward (Author) / LaBelle, Jeffrey (Thesis director) / Santello, Marco (Committee member) / Barrett, The Honors College (Contributor) / Computer Science and Engineering Program (Contributor)
Created2013-12
Description
Improved pancreatic cancer diagnostic technology has the potential to improve patient prognosis by increasing cancer screening rates and encouraging early detection of the cancer. To increase the sensitivity and specificity while decreasing the cost and time investment, the emerging detection method of electrochemical impedance spectroscopy (EIS) was tested to detect two pancreatic cancer specific biomarkers. The antibodies of carcinoembryonic antigen and quiescin sulfhydryl oxidase 1 were immobilized individually to gold disk electrodes and tested for binding to their respective antigens. An AC signal of varying potential and a wide frequency sweep was applied to the electrode system and the resulting imaginary impedance values were analyzed. Based off of the highest slope and R-squared values of the collected impedance values, the optimal binding frequencies of QSOX1 and CEA with their antibodies was determined to be 97.66 Hz and 17.44 Hz, respectively. EIS was also used to test for potential multimarker detection by coimmobilizing anti-CEA and anti-QSOX1 to the surface of gold disk electrodes. Each system's impedance response was correlated to the physiological concentration range of CEA and QSOX1 individually. The resulting impedance and concentration calibration curves had R-squared values of 0.78 and 0.79 for the calculated QSOX1 and CEA, respectively. Both markers showed similar trends between the calculated and actual calibration curves for each marker. The imaginary impedance output lacks two independent peaks for the distinct optimal binding frequencies of both biomarkers after signal subtraction and show a large shift in optimal frequencies. From analyzing the co-immobilization data for the calculated and experimentally determined calibration curves of CEA and QSOX1, both curves had different correlation values between imaginary impedance values and concentration. Add and subtracting the experimental and calculated co-immobilization, QSOX1, and CEA signals suggest an oversaturation of QSOX1 used during the experiments.
ContributorsMalla, Akshara (Co-author) / Murali, Keerthana (Co-author) / LaBelle, Jeffrey (Thesis director) / Lin, Chi-En (Committee member) / Harrington Bioengineering Program (Contributor) / Barrett, The Honors College (Contributor)
Created2017-05
Description
A point of care glucose sensor using electrochemical impedance spectroscopy (EIS) with a glutaraldehyde-linked enzyme shows promise as an effective biosensor platform. This report details the characterization of various factors on optimal binding frequency (OBF) and sensor performance to better prepare the sensor for future experimentation. Utilizing a screen printed carbon electrode, the necessary amount of glucose oxidase was determined to be 10 mg/mL. Binding time trials ranging from 1-3 minutes demonstrated that 1.5 minutes was the optimal binding time. This timeframe produced the strongest impedance response at each glucose concentration. Using this enzyme concentration and binding time, the native OBF of the biosensor was found to be 1.18 Hz using vector analysis. Temperature testing showed little change in OBF in sensors exposed to 4 \u00B0C through 43.3 \u00B0C. Only exposure to 60 \u00B0C resulted in rapid OBF change which was likely due to glucose oxidase becoming denatured. Humidity tests showed little change in OBF and sensor performance between sensors prepared at the humidities of 7.5%, 10.625% and 16.5% humidity. Alternatively, solutions containing common interference molecules such as uric acid, acetaminophen, and ascorbic acid resulted in a highly shifted OBF and drastically reduced signal.
ContributorsMatloff, Daniel (Co-author) / Khanwalker, Mukund (Co-author) / Johns, Jared (Co-author) / LaBelle, Jeffrey (Thesis director) / Pizziconi, Vincent (Committee member) / Lin, Chi (Committee member) / Dean, W.P. Carey School of Business (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2018-12
Description
As of today, there does not exist a cheap diagnostic for lactate for use in trauma centers. $671 billion are spent on trauma accidents and emergency rooms, with money focused on treatments such as YSI and ELISA, costing $1500 and $200, respectively. Gold disk electrodes were used to immobilize lactate dehydrogenase and glucose oxidase, with electrochemical impedance spectroscopy (EIS) used as the method for detection. Two lactate experimental runs were completed with data detailing a linear model and positive correlation for imaginary impedance and concentration, and one glucose experimental run was completed proving that a continuous system can be completed accounting for reaction and consumption using EIS, a process previously not done before.
ContributorsEltohamy, Omar Khaled (Author) / LaBelle, Jeffrey (Thesis director) / Lin, Chi-En (Committee member) / Harrington Bioengineering Program (Contributor, Contributor) / Barrett, The Honors College (Contributor)
Created2017-05