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An understanding of diet habits is crucial in implementing proper management strategies for wildlife. Diet analysis, however, remains a challenge for ruminant species. Microhistological analysis, the method most often employed in herbivore diet studies, is tedious and time consuming. In addition, it requires considerable training and an extensive reference plant

An understanding of diet habits is crucial in implementing proper management strategies for wildlife. Diet analysis, however, remains a challenge for ruminant species. Microhistological analysis, the method most often employed in herbivore diet studies, is tedious and time consuming. In addition, it requires considerable training and an extensive reference plant collection. The development of DNA barcoding (species identification using a standardized DNA sequence) and the availability of recent DNA sequencing techniques offer new possibilities in diet analysis for ungulates. Using fecal material collected from controlled feeding trials on pygmy goats, (Capra hicus), novel DNA barcoding technology using the P6-loop of the chloroplast trnL (UAA) intron was compared with the traditional microhistological technique. At its current stage of technological development, this study demonstrated that DNA barcoding did not enhance the ability to detect plant species in herbivore diets. A higher mean species composition was reported with microhistological analysis (79%) as compared to DNA barcoding (50%). Microhistological analysis consistently reported a higher species presence by forage class. For affect positive species identification, microhistology estimated an average of 89% correct detection in control diets, while DNA barcoding estimated 50% correct detection of species. It was hypothesized that a number of factors, including variation in chloroplast content in feed species and the effect of rumen bacteria on degradation of DNA, influenced the ability to detect plant species in herbivore diets and concluded that while DNA barcoding opens up new possibilities in the study of plant-herbivore interactions, further studies are needed to standardize techniques and for DNA bar-coding in this context.
ContributorsMurphree, Julie Joan (Author) / Miller, William H. (Thesis advisor) / Steele, Kelly (Committee member) / Salywon, Andrew (Committee member) / Arizona State University (Publisher)
Created2012
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Description
Since its inception in 1973, the Endangered Species Act has been met with both praise and criticism. More than 40 years later, the Act is still polarizing, with proponents applauding its power to protect species and critics arguing against its perceived ineffectiveness and potential mismanagement. Recovery plans, which were required

Since its inception in 1973, the Endangered Species Act has been met with both praise and criticism. More than 40 years later, the Act is still polarizing, with proponents applauding its power to protect species and critics arguing against its perceived ineffectiveness and potential mismanagement. Recovery plans, which were required by the 1988 amendments to the Act, play an important role in organizing efforts to protect and recover species under the Act. In 1999, in an effort to evaluate the process, the Society for Conservation Biology commissioned an independent review of endangered species recovery planning. From these findings, the SCB made key recommendations for how management agencies could improve the recovery planning process, after which the Fish and Wildlife Service and the National Marine Fisheries Service redrafted their recovery planning guidelines. One important recommendation called for recovery plans to make threats a primary focus, including organizing and prioritizing recovery tasks for threat abatement. Here, I seek to determine the extent to which SCB recommendations were incorporated into these new guidelines, and if, in turn, the recommendations regarding threats manifested in recovery plans written under the new guidelines. I found that the guidelines successfully incorporated most SCB recommendations, except those that addressed monitoring. As a result, recent recovery plans have improved in their treatment of threats, but still fail to adequately incorporate threat monitoring. This failure suggests that developing clear guidelines for monitoring should be an important priority in future ESA recovery planning.
ContributorsTroyer, Caitlin (Author) / Gerber, Leah (Thesis advisor) / Minteer, Ben (Committee member) / Guston, David (Committee member) / Arizona State University (Publisher)
Created2014
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Description
For the past 30 years wildlife biologists have debated the need of pronghorn antelope (Antilocapra americana) to drink freestanding water (free water). Some have suggested that pronghorn may feed at night to increase preformed water (plant moisture) intake, thus decreasing their dependence on free water. Pronghorn diet composition and nutrient

For the past 30 years wildlife biologists have debated the need of pronghorn antelope (Antilocapra americana) to drink freestanding water (free water). Some have suggested that pronghorn may feed at night to increase preformed water (plant moisture) intake, thus decreasing their dependence on free water. Pronghorn diet composition and nutrient intake is integral to understanding water available to pronghorn through preformed and metabolic sources. The dual purpose of this study was to determine plant composition of pronghorn diets, and to examine whether night feeding provides a water allocation advantage by testing for differences between day and night and modeling free water requirements during biologically critical seasons and years of different precipitation. I determined species composition, selected nutrients, and moisture content of American pronghorn diets on Perry Mesa, Arizona in March, May, June and August of 2008 and 2009. I used microhistological analysis of fecal samples to determine percent plant composition of pronghorn diets. I used forage samples to evaluate the nutrient composition of those diets for moisture, crude protein and structural carbohydrates, and to calculate metabolic water. I used calculations proposed by Fox et al. (2000) to model free water requirements and modified the equations to reflect increased requirements for lactation. Diet analysis revealed that pronghorn used between 67% and 99% forbs and suggested fair range conditions. Preformed water was not significantly different between night and day. Night feeding appeared to be of marginal advantage, providing an average potential 9% preformed water increase in 2008, and 3% in 2009. The model indicated that neither male nor female pronghorn could meet their water requirements from preformed and metabolic water during any time period, season or year. The average free water requirements for females ranged from 0.67 L/animal/day (SE 0.06) in March, 2008 to 3.12 L/animal/day (SE 0.02) in June, 2009. The model showed that American pronghorn on Perry Mesa require access to free water during biological stress periods.
ContributorsTluczek, Melanie (Author) / Miller, William H. (Thesis advisor) / Brown, David E. (Committee member) / Steele, Kelly (Committee member) / Arizona State University (Publisher)
Created2012
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Description
Is it possible to treat the mouth as a natural environment, and determine new methods to keep the microbiome in check? The need for biodiversity in health may suggest that every species carries out a specific function that is required to maintain equilibrium and homeostasis within the oral cavity. Furthermore,

Is it possible to treat the mouth as a natural environment, and determine new methods to keep the microbiome in check? The need for biodiversity in health may suggest that every species carries out a specific function that is required to maintain equilibrium and homeostasis within the oral cavity. Furthermore, the relationship between the microbiome and its host is mutually beneficial because the host is providing microbes with an environment in which they can flourish and, in turn, keep their host healthy. Reviewing examples of larger scale environmental shifts could provide a window by which scientists can make hypotheses. Certain medications and healthcare treatments have been proven to cause xerostomia. This disorder is characterized by a dry mouth, and known to be associated with a change in the composition, and reduction, of saliva. Two case studies performed by Bardow et al, and Leal et al, tested and studied the relationships of certain medications and confirmed their side effects on the salivary glands [2,3]. Their results confirmed a relationship between specific medicines, and the correlating complaints of xerostomia. In addition, Vissink et al conducted case studies that helped to further identify how radiotherapy causes hyposalivation of the salivary glands [4]. Specifically patients that have been diagnosed with oral cancer, and are treated by radiotherapy, have been diagnosed with xerostomia. As stated prior, studies have shown that patients having an ecologically balanced and diverse microbiome tend to have healthier mouths. The oral cavity is like any biome, consisting of commensalism within itself and mutualism with its host. Due to the decreased salivary output, caused by xerostomia, increased parasitic bacteria build up within the oral cavity thus causing dental disease. Every human body contains a personalized microbiome that is essential to maintaining health but capable of eliciting disease. The Human Oral Microbiomics Database (HOMD) is a set of reference 16S rRNA gene sequences. These are then used to define individual human oral taxa. By conducting metagenomic experiments at the molecular and cellular level, scientists can identify and label micro species that inhabit the mouth during parasitic outbreaks or a shifting of the microbiome. Because the HOMD is incomplete, so is our ability to cure, or prevent, oral disease. The purpose of the thesis is to research what is known about xerostomia and its effects on the complex microbiome of the oral cavity. It is important that researchers determine whether this particular perspective is worth considering. In addition, the goal is to create novel experiments for treatment and prevention of dental diseases.
ContributorsHalcomb, Michael Jordan (Author) / Chen, Qiang (Thesis director) / Steele, Kelly (Committee member) / Barrett, The Honors College (Contributor) / College of Letters and Sciences (Contributor)
Created2015-05
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Description
The plateau pika (Ochotona curzoniae), a small burrowing lagomorph that occupies the high alpine grassland ecosystems of the Qinghai-Tibetan Plateau in western China, remains a controversial subject among policymakers and researchers. One line of evidence points to pikas being a pest, which has led to massive attempts to eradicate pika

The plateau pika (Ochotona curzoniae), a small burrowing lagomorph that occupies the high alpine grassland ecosystems of the Qinghai-Tibetan Plateau in western China, remains a controversial subject among policymakers and researchers. One line of evidence points to pikas being a pest, which has led to massive attempts to eradicate pika populations. Another point of view is that pikas are a keystone species and an ecosystem engineer in the grassland ecosystem of the QTP. The pika eradication program raises a difficult ethical and religious dilemma for local pastoralists, and is criticized for not being supported by scientific evidence. Complex interactions between pikas, livestock, and habitat condition are poorly understood. My dissertation research examines underpinning justifications of the pika poisoning program leading to these controversies. I investigated responses of pikas to habitat conditions with field experimental manipulations, and mechanisms of pika population recovery following pika removal. I present policy recommendations based on an environmental ethics framework and findings from the field experiments. After five years of a livestock grazing exclusion experiment and four years of pika monitoring, I found that grazing exclusion resulted in a decline of pika habitat use, which suggests that habitat conditions determine pika population density. I also found that pikas recolonized vacant burrow systems following removal of residents, but that distances travelled by dispersing pikas were extremely short (~50 m). Thus, current pika eradication programs, if allowed to continue, could potentially compromise local populations as well as biodiversity conservation on the QTP. Lethal management of pikas is a narrowly anthropocentric-based form of ecosystem management that has excluded value-pluralism, such as consideration of the intrinsic value of species and the important ecological role played by pikas. These conflicting approaches have led to controversies and policy gridlock. In response, I suggest that the on-going large-scale pika eradication program needs reconsideration. Moderation of stocking rates is required in degraded pika habitats, and Integrated Pest Management may be required when high stocking rate and high pika density coexist. A moderate level of livestock and pika density can be consistent with maintaining the integrity and sustainability of the QTP alpine steppe ecosystem.
ContributorsBadingqiuying (Author) / Smith, Andrew T. (Thesis advisor) / Wu, Jianguo (Committee member) / Minteer, Ben (Committee member) / Anderies, John (Committee member) / Harris, Richard B. (Committee member) / Arizona State University (Publisher)
Created2016
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Description
The issue of wild horse management in the American West has become prevalent in the media recently and management strategies are often criticized and opposed by the public. Horses have been a core feature of American history and culture nearly since the colonization of the western frontier, and popular media

The issue of wild horse management in the American West has become prevalent in the media recently and management strategies are often criticized and opposed by the public. Horses have been a core feature of American history and culture nearly since the colonization of the western frontier, and popular media such as television and movies paint a romantic but often unrealistic picture of wild horses. Land management agencies must balance limited resources with an ever-growing wild horse population in order to properly manage public land so that it retains its ecological integrity and is still able to be used by multiple stakeholders, and they also must endure public criticism throughout the process. I used a photo elicitation survey to gather responses to photographic images of wild horses and determine how the public feels about wild horse management, given that horses are seen as a symbol of freedom and the American West. It was revealed that people who are unfamiliar with the issue still have opinions about how the horses should be managed, and these opinions often mirror what can be found in popular media.
ContributorsDuran, Kiana Alexis (Author) / Minteer, Ben (Thesis director) / Schoon, Michael (Thesis director) / Murphree, Julie (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05