Premature babies are at risk of death from immature lung development. For this reason, pregnant mothers at risk for preterm delivery are administered dexamethasone (DEX), a synthetic glucocorticoid that promotes fetal lung development. However, exposure to DEX in utero is associated with low birth weight and cardiovascular development pathologies. Moreover, our lab found that DEX administration in-utero leads to a sex-specific increase in stress-induced tachycardia in female, but not male offspring. This project seeks to expand on this preliminary finding of the heart by examining local effectors of activity from the sympathetic system (tyrosine hydroxylase and catechol-o-methyltransferase). Tyrosine hydroxylase was measured as it catalyzes the rate limiting step of norepinephrine synthesis while catechol-O- methyltransferase was studied as it catalyzes the degradation of norepinephrine. Acetylcholinesterase was used to measure parasympathetic activity as it catalyzes the degradation of the primary neurotransmitter of the parasympathetic nervous system, acetylcholine. Analyses of sympathetic as well as parasympathetic activity were done to determine influences of in-utero DEX exposure on autonomic regulation in adulthood. Pregnant rats were administered DEX (0.4 mg/kg, i.p.) or vehicle (20% w/v 2-hydroxypropyl ß- cyclodextran) at gestation days 18-21, with euthanasia of offspring occurring at around the time the offspring reached 13-15 weeks of age. Left ventricles and right atria were pulverized, processed and subjected to western blot analysis to determine expression of proteins of interest. Males exposed to DEX in-utero saw a decrease in tyrosine hydroxylase expression in left ventricle and right atrium when compared to vehicle control, a difference not seen with females. In addition, catechol-o-methyltransferase expression was increased in right atria from male, but not female rats. Acetylcholinesterase expression was reduced in the right atria of female, but not male rats. The present findings suggest reduced norepinephrine signaling in the heart of male, but not female DEX-exposed offspring. Given that we have previously found that female, but not male rats exhibit exaggerated stress-induced tachycardia, our current findings suggest that males possess a sex-specific compensatory mechanism allowing the heart to resist increased sympathetic signaling from the brain, one that females do not possess. The underlying mechanics of this proposed mechanism are unclear, and further investigation is needed in this subject to determine the significance of the findings from our study.

As political campaigning becomes increasingly digital and data-driven, data privacy has become a question of democratic governance. Yet, Congress has yet to pass a comprehensive federal data privacy law and even the strongest subnational data privacy laws exempt political campaigns from regulation. <br/><br/>This thesis examines how data privacy laws impact data-driven and digital political campaigning. Specifically, it investigates what information is incorporated into the political data ecosystem, how data privacy laws regulate the collection of this data, and how actors in the political data ecosystem respond to these laws. It examines both sector-specific federal law and subnational data protection regulation through a case study of California. This research suggests that although the California Consumer Privacy Act and California Privacy Rights Act are landmark steps in American data protection, subnational data privacy law remains inhibited by the federal market-based approach.

Within the last decade, there has been a lot of hype surrounding the potential medical applications of artificial intelligence (AI) and machine learning (ML) technologies. During the same timespan, big tech companies such as Microsoft, Apple, Amazon, and Google have entered the healthcare market as developers of health-based AI and ML technologies. This project aims to create a comprehensive map of the existing health-AI market landscape for the standard biotech reader and to provide a critical commentary on the existing market structure.

It is a fact of modern food processing that the majority of products contain one or multiple food additives. Yet, while these additives see great abundance of use, the average consumer has relatively little knowledge about them and, more often than not, a negative opinion of their inclusion. This piece explores the discrepancy between these two realities by delving into the origins, histories of use, health effects, and misconceptions that surround a number of modern food additives, exploring along the way the social changes and regulatory history that brought about the legal landscape of food safety in the United States. Ten author-developed recipes are included at the end to encourage not only a conceptual, but also a practical familiarity with these same food additives.