Managed Aquifer Recharge is an increasingly prevalent solution to sustain water availability in arid regions. Recharge of groundwater resources using treated wastewater effluent is one type of managed aquifer recharge that offers long-term sustainable water management. However, there are some concerns regarding the reuse of wastewater and its potential to increase exposures to antibiotic resistant bacteria and antibiotic resistance genes that could affect human health. Antibiotic resistance genes can confer the ability for bacteria to resist antibacterial treatment, rendering their presence in water supplies as an area of research needed to evaluate where environmental “hot spots” of potential antibiotic resistance disseminate. To evaluate the occurrence of antibiotic resistant bacteria and antibiotic resistance genes, sampling of an Arizona managed aquifer recharge facility was performed, with target antibiotic resistance genes measured using quantitative polymerase chain reaction. The occurrence of antibiotic resistance genes was evaluated at several sampling wells and in sediments to examine trade-offs between water quantity benefits and water quality issues. The goal of this work is to inform management operations for secure water quality in the face of climate change.
Bacteriophages are the most numerous type of organisms in the world. These viruses have the ability to destroy bacteria that they specifically match. Phage therapy shows great promise to become a solution to bacterial infections and antibiotic resistance. To ensure that younger generations are aware of this field of research, we created a website to educate them. The website contains lessons and a game that will encourage confidence, curiosity, and understanding of bacteriophages.
for Unmanned Aerial Vehicles.
Towards enabling a UAV to autonomously sense and avoid moving obstacles, this thesis makes the following contributions. Initially, an image-based reactive motion planner is developed for a quadrotor to avoid a fast approaching obstacle. Furthermore, A Dubin’s curve based geometry method is developed as a global path planner for a fixed-wing UAV to avoid collisions with aircraft. The image-based method is unable to produce an optimal path and the geometry method uses a simplified UAV model. To compensate
these two disadvantages, a series of algorithms built upon the Closed-Loop Rapid Exploratory Random Tree are developed as global path planners to generate collision avoidance paths in real time. The algorithms are validated in Software-In-the-Loop (SITL) and Hardware-In-the-Loop (HIL) simulations using a fixed-wing UAV model and in real flight experiments using quadrotors. It is observed that the algorithm enables a UAV to avoid moving obstacles approaching to it with different directions and speeds.