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- Creators: Harrington Bioengineering Program
- Creators: School of Nutrition and Health Promotion
- Status: Published
Description
One of the most prominent biological challenges for the field of drug delivery is the blood-brain barrier. This physiological system blocks the entry of or actively removes almost all small molecules into the central nervous system (CNS), including many drugs that could be used to treat diseases in the CNS. Previous studies have shown that activation of the adenosine receptor signaling pathway through the use of agonists has been demonstrated to increase BBB permeability. For example, regadenoson is an adenosine A2A receptor agonist that has been shown to disrupt the BBB and allow for increased drug uptake in the CNS. The goal of this study was to verify this property of regadenoson. We hypothesized that co-administration of regadenoson with a non-brain penetrant macromolecule would facilitate its entry into the central nervous system. To test this hypothesis, healthy mice were administered regadenoson or saline concomitantly with a fluorescent dextran solution. The brain tissue was either homogenized to measure quantity of fluorescent molecule, or cryosectioned for imaging with confocal fluorescence microscopy. These experiments did not identify any significant difference in the amount of fluorescence detected in the brain after regadenoson treatment. These results contradict those of previous studies and highlight potential differences in injection methodology, time windows, and properties of brain impermeant molecules.
ContributorsWohlleb, Gregory Michael (Author) / Sirianni, Rachael (Thesis director) / Stabenfeldt, Sarah (Committee member) / Barrett, The Honors College (Contributor) / Harrington Bioengineering Program (Contributor)
Created2015-05
Description
The global population over the age of 60 is estimated to rise to 23% by 2050 only increase the prevalence of functional neurological disorders and stroke. Increase in cases of functional neurological disorders and strokes will place a greater burden on the healthcare industry, specifically physical therapy. Physical therapy is vital for a patient’s recovery of motor function which is time demanding and taxing on the physical therapist. Wearable robotics have been proven to improve functional outcomes in gait rehabilitation by providing controlled high dosage and high-intensity training. Accurate control strategies for assistive robotic exoskeletons are vital for repetitive high precisions assistance for cerebral plasticity to occur.
This thesis presents a preliminary determination and design of a control algorithm for an assistive ankle device developed by the ASU RISE Laboratory. The assistive ankle device functions by compressing a spring upon heel strike during gait, remaining compressed during mid-stance and then releasing upon initiation of heel-off. The relationship between surface electromyography and ground reactions forces were used for identification of user-initiated heel-off. The muscle activation of the tibialis anterior combined with the ground reaction forces of the heel pressure sensor generated potential features that will be utilized in the revised control algorithm for the assistive ankle device. Work on this project must proceed in order to test and validate the revised control algorithm to determine its accuracy and precision.
This thesis presents a preliminary determination and design of a control algorithm for an assistive ankle device developed by the ASU RISE Laboratory. The assistive ankle device functions by compressing a spring upon heel strike during gait, remaining compressed during mid-stance and then releasing upon initiation of heel-off. The relationship between surface electromyography and ground reactions forces were used for identification of user-initiated heel-off. The muscle activation of the tibialis anterior combined with the ground reaction forces of the heel pressure sensor generated potential features that will be utilized in the revised control algorithm for the assistive ankle device. Work on this project must proceed in order to test and validate the revised control algorithm to determine its accuracy and precision.
ContributorsGaytan-Jenkins, Daniel Rinaldo (Author) / Zhang, Wenlong (Thesis director) / Tyler, Jamie (Committee member) / Harrington Bioengineering Program (Contributor, Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
The research being proposed would develop an objective test for handedness analyzing circle-drawing movements performed with the dominant arm versus non-dominant arm. Handedness is a unique and exceptional characteristic of human beings which impacts society on an individual basis that has far-reaching influence. Its correlation and possible causation has been studied and implied in everything from mental disorders (Deep-Soboslay et al. 2010) to advanced biological processes (Driscoll, Kei, & McPherson, 2002). Despite the importance of handedness, there are many faults surrounding the widely used methods for determining and classifying handedness. The most common of these, the Edinburgh Handedness Inventory, especially suffers from reporter bias, possibly confusing categories and instructions, and underestimating ambidextrous or mixed handedness. Research done by R.L. Sainburg of Penn State and N. Dounskaia of Arizona State University points to a possible method of measuring handedness. The findings of these studies show show that the dominant arm to perform better in drawing movements than the non-dominant arm. It is proposed that an objective test could be developed for handedness using circle-drawing tasks. A participant would draw circles with both arms, these movements would be analyzed to show which arm was dominant by showing which arm made the more perfect circle. By developing an objective test, handedness could be more properly classified and assessed, helping aid research and understanding in how handedness affects humans.
ContributorsKleisler, Kevin C. (Author) / Dounskaia, Natalia (Thesis director) / Ringenbach, Shannon (Committee member) / Wang, Wanyue (Committee member) / Barrett, The Honors College (Contributor) / School of Nutrition and Health Promotion (Contributor)
Created2013-12
Description
Although the sport and exercise of running has a great amount of benefits to anyone's health, there is a chance of injury that can occur. There are many variables that can contribute to running injury. However, because of the vast amount of footsteps a frequent runner takes during their average run, foot strike pattern is a significant factor to be investigated in running injury research. This study hypothesized that due to biomechanical factors, runners that exhibited a rear foot striking pattern would display a greater incidence of chronic lower extremity injury in comparison to forefoot striking counterparts. This hypothesis would support previous studies conducted on the topic. Student-athletes in the Arizona State University- Men's and Women's Track & Field program, specifically those who compete in distance events, were given self reporting surveys to provide injury history and had their foot strike patterns analyzed through video recordings. The survey and analysis of foot strike patterns resulted in data that mostly followed the hypothesized pattern of mid-foot and forefoot striking runners displaying a lower average frequency of injury in comparison to rear foot strikers. The differences in these averages across all injury categories was found to be statistically significant. One category that displayed the most supportive results was in the average frequency of mild injury. This lead to the proposed idea that while foot strike patterns may not be the best predictor of moderate and severe injuries, they may play a greater role in the origin of mild injury. Such injuries can be the gateway to more serious injury (moderate and severe) that are more likely to have their cause in other sources such as genetics or body composition for example. This study did support the idea that foot strike pattern can be the main predictor in incidence of running injuries, but also displayed that it is one of many major factors that contribute to injuries in runners.
ContributorsBaker-Slama, Garrett Richard (Author) / Harper, Erin (Thesis director) / Cataldo, Donna (Committee member) / Wilson, Jeffrey (Committee member) / Barrett, The Honors College (Contributor) / School of Nutrition and Health Promotion (Contributor)
Created2014-05
Description
Research on joint control during arm movements in adults has led to the development of the Leading Joint Hypothesis (LJH), which states that the central nervous system takes advantage of interaction torque (IT) and muscle torque (MT) to produce movements with maximum efficiency in the multi-jointed limbs of the human body. A gap in knowledge exists in determining how this mature pattern of joint control develops in children. Prior research focused on the kinematics of joint control for children below the age of three; however, not much is known about interjoint coordination with respect to MT and IT in school-aged children. In the present study, joint control at the shoulder, elbow, and wrist during drawing of five shapes was investigated. A random sample of nine typically developing children ages 6 to 12 served as subjects. The task was to trace with the index finger a template placed on a horizontal table. The template consisted of a circle, horizontal, vertical, right-diagonal, and left-diagonal line. Analysis of muscle torque contribution (MTC) revealed the individual roles of MT and IT in the shoulder, elbow, and wrist joints. During drawing of the horizontal line, which requires the most difficult joint control pattern in adults because it does not allow the use of IT for joint rotation, joint control was found to change through development. For the youngest children, the function of elbow MT modified to suppress IT, thereby producing large elbow rotation. The oldest children simplified this by using the shoulder as the principal joint of movement production and with decreased assistance from the elbow. For the other four drawing movements, differences in the pattern of joint control used by all of the subjects was unaffected by an increase in age. Overall, the results suggest that in children above 6 years of age, minor changes in joint control occur during drawing of relatively simple movements. The limited effect of age that was observed could be related to the restriction of movements to the horizontal plane. For a future study, three-dimensional movements that provide more freedom in joint control due to redundancy of degrees of freedom could be more informative about developmental changes in joint coordination.
ContributorsKemmou, Nadaa (Co-author) / Way, Victoria (Co-author) / Dounskaia, Natalia (Thesis director) / Vidt, Meghan (Committee member) / School of Nutrition and Health Promotion (Contributor) / Department of Psychology (Contributor) / Barrett, The Honors College (Contributor)
Created2016-12
Description
Neurological disorders are difficult to treat with current drug delivery methods due to their inefficiency and the lack of knowledge of the mechanisms behind drug delivery across the blood brain barrier (BBB). Nanoparticles (NPs) are a promising drug delivery method due to their biocompatibility and ability to be modified by cell penetrating peptides, such as transactivating transciptor (TAT) peptide, which has been shown to increase efficiency of delivery. There are multiple proposed mechanisms of TAT-mediated delivery that also have size restrictions on the molecules that can undergo each BBB crossing mechanism. The effect of nanoparticle size on TAT-mediated delivery in vivo is an important aspect to research in order to better understand the delivery mechanisms and to create more efficient NPs. NPs called FluoSpheres are used because they come in defined diameters unlike polymeric NPs that have a broad distribution of diameters. Both modified and unmodified 100nm and 200nm NPs were able to bypass the BBB and were seen in the brain, spinal cord, liver, and spleen using confocal microscopy and a biodistribution study. Statistically significant differences in delivery rate of the different sized NPs or between TAT-modified and unmodified NPs were not found. Therefore in future work a larger range of diameter size will be evaluated. Also the unmodified NPs will be conjugated with scrambled peptide to ensure that both unmodified and TAT-modified NPs are prepared in identical fashion to better understand the role of size on TAT targeting. Although all the NPs were able to bypass the BBB, future work will hopefully provide a better representation of how NP size effects the rate of TAT-mediated delivery to the CNS.
ContributorsCeton, Ricki Ronea (Author) / Stabenfeldt, Sarah (Thesis director) / Sirianni, Rachael (Committee member) / Harrington Bioengineering Program (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
The effect of conflicting sensorimotor memories on optimal force strategies was explored. Subjects operated a virtual object controlled by a physical handle to complete a simple straight-line task. Perturbations applied to the handle induced a period of increased error in subject accuracy. After two blocks of 33 trials, perturbations switched direction, inducing increased error from the previous trials. Subjects returned after a 24-hour period to complete a similar protocol, but beginning with the second context and ending with the first. Interference from the first context on each day caused an increase in initial error for the second (P < 0.05). Following the rest period, subjects showed retention of the sensorimotor memory from the previous day through significantly decreased initial error (P = 3x10-6). However, subjects showed an increase in forces for each new context resulting from a sub-optimal motor strategy. Higher levels of total effort (P < 0.05) and a lack of separation between force values for opposing and non-opposing digits (P > 0.05) indicated a strategy that used more energy to complete the task, even when rates of learning appeared identical or improved. Two possible mechanisms for this lack of energy conservation have been proposed.
ContributorsSmith, Michael David (Author) / Santello, Marco (Thesis director) / Kleim, Jeffrey (Committee member) / Harrington Bioengineering Program (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
Medial compartment knee osteoarthritis (OA) is a disease whose severity has been associated with the peak adduction moment during walking (pKAM). Unfortunately, measuring patients' pKAM to track their therapy progress involves the use of a gait laboratory which is expensive and time intensive. This study aimed to develop and assess a regression method to predict the pKAM using only plantar pressure measurements. This approach could greatly reduce the burden of evaluating pKAM.
ContributorsThomas, Kevin Andrew (Author) / Hinrichs, Richard (Thesis director) / Harper, Erin (Committee member) / Favre, Julien (Committee member) / Barrett, The Honors College (Contributor) / Harrington Bioengineering Program (Contributor)
Created2014-05
Description
Human walking is a complex and rhythmical activity that comprises of the brain, nerves and muscles. Neuromuscular disorder (NMD) is a broad term that refers to conditions that affect the proper use of muscles and nervous system, thus also impairing the walking or gait cycle of an individual. The improper gait cycle might be attributed to the lack of force produced at the toe-off stage. This project addresses if it is possible to create an OpenSim model to find the ideal time and force magnitude needed of an assistive force ankle device to improve gait patterns in individuals with NMD.
ContributorsRivera, Jose Luis (Author) / Zhang, Wenlong (Thesis director) / Lockhart, Thurmon (Committee member) / Harrington Bioengineering Program (Contributor, Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
Over the past 30 years the use of graphene has been increasing at a rapid rate. The reason why graphene has become more popular is because it is starting to be understood better, and researchers are starting to recognize graphene’s unique properties. Graphene is a single atomic layer of graphite, and graphite is a three-dimensional cube base structure of carbon. Graphite has a high conductivity rate, and graphene has an even higher conductivity, meaning that graphene makes for an excellent resistor in any hardware system. Graphene is flexible, has high durability, and can vary in resistance based on its shape (Sharon 2015). With graphene being able to change its resistivity, it can act as different types of sensors. These sensors include measuring pressure, resistance, force, strain, and angle. One problem across the globe is that patients have arthritis, decaying bone density, and injuries which can easily go mistreated or not treated at all. It can be hard to determine the severity of injuries in joints by observation of the patient. There are tools and equipment that will allow a doctor to track the force and degrees of motion of certain joints, but they are mostly limited to hospitals. With graphene acting as a sensor it can be embedded into casts, braces, and even clothing. With a mobile sensor that relays accurate and continuous data to a doctor they can more precisely determine a therapy or recovery time that will better suit the patients’ needs. In this project the graphene was used to measure the angle of a patient’s wrist while they were wearing a wrist brace. From the data collected, the graphene was able to track the user’s movement of their wrist as they moved it in a single direction. The data showed the angle of the wrist ranging from zero degrees to 90 degrees. This proves that graphene can shape the way biosensing is accomplished. Biodynamics is a growing field, and with more injuries everyday it is important to study graphene and how it can be used to diagnose and prevent injuries related to joints. Graphene can be used as a biosensor which can then be implemented into a brace to allow for accurate biodynamic tracking.
ContributorsSweeten, William (Author) / Lockhart, Thurmon (Thesis director) / Helms Tillery, Stephen (Committee member) / Harrington Bioengineering Program (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05