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- Creators: School of Sustainability
- Member of: Barrett, The Honors College Thesis/Creative Project Collection
Description
Dengue virus infects millions of people every year. Yet there is still no vaccine available to prevent it. Here we use a neutralizing epitope determinant on the dengue envelope (E) protein as an immunogen to be vectored by a measles virus (MV) vaccine. However the domain III (DIII) of the dengue 2 E protein is too small to be immunogenic by itself. In order for it to be displayed on a larger particle, it was inserted into the amino terminus of small hepatitis B surface antigen (HBsAg, S) coding sequence. To generate the recombinant MV vector and verify the efficiency of this concept, a reverse genetics system was used where the MV vectors express one or two additional transcription units to direct the assembly of hybrid HBsAg particles. Two types of recombinant measles virus were produced: pB(+)MVvac2(DIII-S,S)P and pB(+)MVvac2(DIII-S)N. Virus recovered from pB(+)MVvac2(DIII-S,S)P was viable. An ELISA assay was performed to demonstrate the expression and secretion of HBsAg. Supernatant from MVvac2(DIII-S,S)P infected cells confirmed that hybrid HBsAg-domain III particles with a density similar to traditional HBsAg particles were released. Characteristics of the subviral particle have been analyzed for the successful incorporation of domain III. The replication fitness of the recombinant MV was evaluated using multi-step growth kinetics and showed reduced replication fitness when compared to the parental strain MVvac2. This demonstrates that viral replication is hindered by the addition of the two inserts into MV genome. Further analysis of MVvac2(DIII-S)N is needed to justify immune response studies in a small animal model using both of the generated recombinant vectors.
ContributorsHarahap, Indira Saridewi (Author) / Reyes del Valle, Jorge (Thesis director) / Hogue, Brenda (Committee member) / Misra, Rajeev (Committee member) / Barrett, The Honors College (Contributor) / T. Denny Sanford School of Social and Family Dynamics (Contributor) / School of Human Evolution and Social Change (Contributor) / School of Life Sciences (Contributor)
Created2014-05
Description
The objective of this thesis was to determine whether Zika Virus (ZIKV) can be effectively inactivated by Selective Photonic Disinfection (SEPHODIS) and determine whether key proteins involved in the infection process are preserved, making SEPHODIS a possible source for vaccine development. As of January 2018, there have been 3,720 confirmed cases of Congenital Zika Syndrome in infants, making a Zika Vaccine a high priority (Mitchell, 2018). SEPHODIS is a process that involves prolonged exposure of an object to a pulsing laser which can render it ineffective. Initially, ZIKV was subjected to laser inactivation for 6 hours, then a plaque assay was performed on both laser-treated and control samples. ZIKV was inactivated two-fold? after laser treatment, when compared with control, as indicated by the plaque assay results. Additionally, both samples were submitted to ELISA to evaluate antigenicity with a panel of monoclonal and human sera. As a second control, virus inactivated by formaldehyde (2%) was used. ELISA results showed that antigenicity of some proteins were preserved while others were probably disturbed. However, ELISA results show that ZIKV envelope protein (E-protein), the protein responsible for viral entry into cells, was effectively preserved after laser-treatment, implying that if laser parameters were tweaked to obtain more complete inactivation, then SEPHODIS may be an appropriate source for the development of a vaccine.
ContributorsViafora, Ataiyo Blue (Author) / Johnston, Stephen (Thesis director) / Tsen, Kong-Thon (Committee member) / School of Life Sciences (Contributor) / School of Sustainability (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
The United States has been facing a resurgence of vaccine preventable infectious diseases. Non-medical vaccination exemptions (NMEs) which include religious exemptions and philosophical vaccine exemptions are contributing factors in state vaccination rates dropping. The policies surrounding such exemptions vary from state to state. Some states with higher rates of nonmedical vaccine exemptions are dealing with repercussions for this including vaccination rates falling below desired herd immunity and thus putting vulnerable populations such as those who are immunocompromised, too young for vaccination and the elderly at a higher risk.
This thesis aims to examine vaccine preventable re-emerging infectious diseases in the United States with the objective of reaching vaccine hesitant populations and providing them with the tools to make informed decisions to seek out immunizations. This will be done by exploring five different diseases and infections, discussing why some individuals feel hesitant to get immunizations, examining how nonmedical vaccine exemptions are correlated to increased cases of disease outbreaks, looking into state laws specifically focused on countering nonmedical vaccine exemptions and the steps that can be taken moving forward.
This thesis aims to examine vaccine preventable re-emerging infectious diseases in the United States with the objective of reaching vaccine hesitant populations and providing them with the tools to make informed decisions to seek out immunizations. This will be done by exploring five different diseases and infections, discussing why some individuals feel hesitant to get immunizations, examining how nonmedical vaccine exemptions are correlated to increased cases of disease outbreaks, looking into state laws specifically focused on countering nonmedical vaccine exemptions and the steps that can be taken moving forward.
ContributorsUmar, Syeda (Author) / Jehn, Megan (Thesis director) / Glegziabher, Meskerem (Committee member) / School of Human Evolution & Social Change (Contributor) / School of Sustainability (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05