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RFID Chip for DNA Tracking Identification

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A primary need of Forensic science is to individualize missing persons that cannot be identified after death. With the use of advanced technology, Radio Frequency Identification (RFID) implant chips can drastically improve digital tracking and enable robust biological and legal

A primary need of Forensic science is to individualize missing persons that cannot be identified after death. With the use of advanced technology, Radio Frequency Identification (RFID) implant chips can drastically improve digital tracking and enable robust biological and legal identification. In this paper, I will discuss applications between different microchip technologies and indicate reasons why the RFID chip is more useful for forensic science. My results state that an RFID chip is significantly more capable of integrating a mass volume of background information, and can utilize implanted individuals’ DNA profiles to decrease the missing persons database backlogs. Since today’s society uses a lot of digital devices that can ultimately identify people by simple posts or geolocation, Forensic Science can harness that data as an advantage to help serve justice for the public in giving loved ones closure.

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2021-05

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Exploiting bioparticles: from new properties of liposomes to novel applications of bioaerosol analysis

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Bioparticles comprise a diverse amount of materials ubiquitously present in nature. From proteins to aerosolized biological debris, bioparticles have important roles spanning from regulating cellular functions to possibly influencing global climate. Understanding their structures, functions, and properties provides

Bioparticles comprise a diverse amount of materials ubiquitously present in nature. From proteins to aerosolized biological debris, bioparticles have important roles spanning from regulating cellular functions to possibly influencing global climate. Understanding their structures, functions, and properties provides the necessary tools to expand our fundamental knowledge of biological systems and exploit them for useful applications. In order to contribute to this efforts, the work presented in this dissertation focuses on the study of electrokinetic properties of liposomes and novel applications of bioaerosol analysis. Using immobilized lipid vesicles under the influence of modest (less than 100 V/cm) electric fields, a novel strategy for bionanotubule fabrication with superior throughput and simplicity was developed. Fluorescence and bright field microscopy was used to describe the formation of these bilayer-bound cylindrical structures, which have been previously identified in nature (playing crucial roles in intercellular communication) and made synthetically by direct mechanical manipulation of membranes. In the biological context, the results of this work suggest that mechanical electrostatic interaction may play a role in the shape and function of individual biological membranes and networks of membrane-bound structures. A second project involving liposomes focused on membrane potential measurements in vesicles containing trans-membrane pH gradients. These types of gradients consist of differential charge states in the lipid bilayer leaflets, which have been shown to greatly influence the efficacy of drug targeting and the treatment of diseases such as cancer. Here, these systems are qualitatively and quantitatively assessed by using voltage-sensitive membrane dyes and fluorescence spectroscopy. Bioaerosol studies involved exploring the feasibility of a fingerprinting technology based on current understanding of cellular debris in aerosols and arguments regarding sampling, sensitivity, separations and detection schemes of these debris. Aerosolized particles of cellular material and proteins emitted by humans, animals and plants can be considered information-rich packets that carry biochemical information specific to the living organisms present in the collection settings. These materials could potentially be exploited for identification purposes. Preliminary studies evaluated protein concentration trends in both indoor and outdoor locations. Results indicated that concentrations correlate to certain conditions of the collection environment (e.g. extent of human presence), supporting the idea that bioaerosol fingerprinting is possible.

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2011

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A search for man's meaning: examining manhood from the margins of gender and orientation

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While numerous studies have examined the nature of masculinity, scholars seldom seek to determine the meaning of manhood or to explore which types of individuals are culturally permitted to call themselves men. One scholarly approach suggests that the meaning of

While numerous studies have examined the nature of masculinity, scholars seldom seek to determine the meaning of manhood or to explore which types of individuals are culturally permitted to call themselves men. One scholarly approach suggests that the meaning of a cultural category can best be illuminated through examining marginalized examples within that category. Based on this assumption, this project illuminates cultural understandings of manhood in the United States by examining the experience of men within two marginalized categories--gay and transsexual--who have often found themselves fighting for the right to call themselves men at a time when hegemonic assumptions about manhood have required that one had been designated male at birth, claims a heterosexual orientation, and exhibits characteristics that are stereotypically masculine. For gay men who were born male, social marginalization could result from one's gay orientation as well as from a perceived lack of masculine traits. For some transsexual gay men, all three of the traditional markers of manhood may be absent or deemed insufficient. This scenario calls into question what it is that all men have in common if the concept of manhood is to be associated with any stable definition. Within rhetorical analysis, the concept of textual fragmentation suggests that a rhetorical critic performs an analysis of a text by examining dense textual fragments; the critic's audience members then produce what they perceive to be a finished discourse in their own minds. Along these lines, this project illuminates the concept of manhood by examining dense textual fragments found within mass media representations and personal narratives, and concludes that one's manhood is determined based on the degree to which one identifies with others who call themselves men. Therefore, manhood can best be framed, not as a specific identity with a stable definition, but as a body of intersecting identifications specific to a particular cultural location and time period. As such, it is linked to cultural systems of power and oppression, illustrating that the claim to manhood as an identity is a rhetorical act that is not free from controversy.

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Date Created
2012

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Application of recognition tunneling in single molecule identification

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Single molecule identification is one essential application area of nanotechnology. The application areas including DNA sequencing, peptide sequencing, early disease detection and other industrial applications such as quantitative and quantitative analysis of impurities, etc. The recognition tunneling technique we have

Single molecule identification is one essential application area of nanotechnology. The application areas including DNA sequencing, peptide sequencing, early disease detection and other industrial applications such as quantitative and quantitative analysis of impurities, etc. The recognition tunneling technique we have developed shows that after functionalization of the probe and substrate of a conventional Scanning Tunneling Microscope with recognition molecules ("tethered molecule-pair" configuration), analyte molecules trapped in the gap that is formed by probe and substrate will bond with the reagent molecules. The stochastic bond formation/breakage fluctuations give insight into the nature of the intermolecular bonding at a single molecule-pair level. The distinct time domain and frequency domain features of tunneling signals were extracted from raw signals of analytes such as amino acids and their enantiomers. The Support Vector Machine (a machine-learning method) was used to do classification and predication based on the signal features generated by analytes, giving over 90% accuracy of separation of up to seven analytes. This opens up a new interface between chemistry and electronics with immediate implications for rapid Peptide/DNA sequencing and molecule identification at single molecule level.

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Date Created
2014

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Elementary teachers' concerns regarding students showing characteristics of a chromosomal disorder

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The presence of certain chromosomal disorders is not always immediately apparent at birth. Children with relatively high-incidence, but non-heritable disorders may receive delayed identification due to the sometimes subtle manifestation of their disorder. Delayed identification may result in various undesirable

The presence of certain chromosomal disorders is not always immediately apparent at birth. Children with relatively high-incidence, but non-heritable disorders may receive delayed identification due to the sometimes subtle manifestation of their disorder. Delayed identification may result in various undesirable outcomes for affected children and their families. In addition to parents, teachers can be valuable participants in the identification process. Chromosomal disorders are associated with generally predictable physical and behavioral characteristics, known as phenotype. In the present study, the influence of phenotype on teachers' student-related concerns was examined. Teachers looked at a photo and read a vignette about a fictional elementary-age student who, although not identified, showed varying degrees of the Turner syndrome phenotype. A follow-up questionnaire indicated significantly greater concerns when a student showed many versus few characteristics of behavioral phenotype. However, the effect of morphological phenotype on teacher responses was not significant. The implications for identification of chromosomal disorders are discussed.

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Date Created
2013

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Adaptive talent management for project professionals: early identification of future industry leaders

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The workforce demographics in the United States are rapidly changing. According to census information, 35% of working adults are project to retire within the next 20 years. The construction is being particularly affected by this demographic shift as fewer employees

The workforce demographics in the United States are rapidly changing. According to census information, 35% of working adults are project to retire within the next 20 years. The construction is being particularly affected by this demographic shift as fewer employees are entering into the industry. This shift is especially bad among project professionals within the industry. The response to these changing demographics depends on how companies manage their talent and plan for successions. In order to investigate this workforce problem in the construction industry, the author has partnered with an expert panel of human resource executives from various companies in the construction industry. This research seeks to investigate methods in which construction companies can identify high potential project leaders early on in their careers through quantitative methodologies. The author first validated the research problem by gathering demographic data from six U.S. construction companies varying in size and industry expertise. As a result of analyzing information from 2,294 construction employees in the project management career path, the authors have found that 58% of these individuals are projected to retire within the next 12 years. The author also conducted a detailed literature review and six company interviews to investigate current succession planning practices in the industry. The results show that very few companies have contingency plans for early to mid-level employees. Lastly, the author conducted 76 employee psychological evaluations to measure personality and behavior traits. These traits were then compared to supervisory performance reviews of these employees. The results of this comparison suggest that high potential employees tend to showcase previous leadership experience and also tend to be more outspoken and are also able to separate their emotional bias from business decisions. Using these findings, the author provides an interview tool that employers can use to expand their talent pool in order to identify high potential candidates that may have been previously overlooked. The author recommends additional research in further developing the use of quantitative tools to evaluate early-career employees in order to more efficiently align resources within the shrinking talent pool.

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Date Created
2017

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Egalitarian socialization and subjective well-being in multiracial individuals: a moderated mediation analysis

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Scholarly interest in racial socialization is growing, but researchers' understanding of how and when racial socialization relates to subjective well-being is underdeveloped, particularly for multiracial populations. The present study investigated the possibility that the relationship of racial socialization to subjective

Scholarly interest in racial socialization is growing, but researchers' understanding of how and when racial socialization relates to subjective well-being is underdeveloped, particularly for multiracial populations. The present study investigated the possibility that the relationship of racial socialization to subjective well-being is mediated by racial identification and that this mediation depends on physical racial ambiguity. Specifically, the proposed study used a moderated mediation model to examine whether the indirect relation of egalitarian socialization to subjective well-being through racial identification is conditional on physical racial ambiguity among 313 multiracial individuals. Results suggested egalitarian socialization was positively correlated with subjective well-being. The results provided no support for the moderated mediation hypothesis. The present study examined the complex interaction between racial socialization, racial identification, physical racial ambiguity, and subjective well-being among multiracial individuals. Despite receiving no support for the moderated mediation hypothesis, this research helped to further explicate a distinct pathway through which egalitarian socialization impacts well-being through racial identification for multiracial individuals independent of physical racial ambiguity.

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2016

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Learning Disabilities or Language Proficiency? Mapping a School’s Understanding of English Learners’ (In)competence

Description

Special education identification processes related to English language learners (ELs) in the United States have puzzled the field for decades. The phenomenon of referrals, the first step toward identification, is complex since it requires deciphering the root cause of students’

Special education identification processes related to English language learners (ELs) in the United States have puzzled the field for decades. The phenomenon of referrals, the first step toward identification, is complex since it requires deciphering the root cause of students’ learning struggles—e.g., second language (L2) factors, the possibility of a learning disability (LD), or the combination of multiple other influences. To investigate the various influences contributing to learning difficulties, I centered this study on three potential sources, individual, institutional, and interpersonal. I aimed to answer, how did sociocultural influences mediate a teacher’s understanding of ELs’ competence? How did sociocultural influences mediate whether a teacher referred ELs to special education services? Using a cultural-historical theoretical approach, I sought deeper theoretical and empirical understandings into how institutional factors (e.g., tiered intervention contexts, policies), combined with other influences, mediated ELs’ referral decisions. I used a multiple parallel case study design following two fifth-grade ELs who faced the possibility of a referral. Interested in the interpersonal domain (e.g., interactions and communication among people), I zoomed in to a local process, student-teacher conferences to examine how classroom processes shaped teachers’ thoughts of students’ competence, and ultimately, referral decisions. I video-recorded teacher-student conference sessions over 14 weeks, and audio-recorded viewing sessions of the recorded conferences to understand teacher and student interpretations of learning competence. To understand how other dimensions (individual and institutional) contributed to teachers’ overall views about the student competence, I interviewed parents and school personnel, wrote observational field notes, and examined archival documents related to student learning over the entire fifth-grade year. I used inductive and iterative qualitative analytical approaches to craft the findings. My findings reaffirmed the complexity involved in finalizing ELs’ referral decisions. I found cultural factors intertwined with structural forces, driving students’ special education candidacies in divergent directions: one evaluated (LD); the other, retained. I also found the referral decisions were based on narrow understandings of learning and behaviors, lack of attention to students’ L2 needs, and faulty and overpowering structural forces which undermined teacher’s professional opinions about the referrals. These findings have implications for research, practice, and policy.

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Date Created
2020

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Combatting Assumption in a Fluid Myth Classroom

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The need to draw a more explicit connection between literature and the daily lives of students has become an increasingly pressing issue. Preeminent literary scholars have long argued that the design of many undergraduate classrooms only engages the student with

The need to draw a more explicit connection between literature and the daily lives of students has become an increasingly pressing issue. Preeminent literary scholars have long argued that the design of many undergraduate classrooms only engages the student with literature to a degree that, long term, does not produce habits of criticism that engage students with wider contexts of conflict. The yield instead primarily takes place in a classroom. Leading scholars tend to draw connections of value between the work they are teaching and the lives of students by focusing on how they negotiate specific power discourses. However, placing an emphasis on having habits of criticism function regarding specific biases in contexts restricts the kinds of conflict students are prepared to negotiate. To encourage a habit of critical thinking in undergraduate students that can be applied to any context of conflict and bias, a vocabulary on language failure should be taught and analyzed through its implications in origin myths that explain and justify division. Language failure, or the failure of symbols to represent subjects in their full capacity, is a concept and theory introduced by Kenneth Burke to examine conflict at a conceivable root. Burke suggests that language failure is the core of misrepresentation and conflict and is inevitably the result of any ‘identification’, or selection of meaning that is assigned to symbols. Identifications are selections of meaning and conceptions of value that organize bodies towards a social purpose, under a limited perspective. The danger of language failure is present when it goes unacknowledged. In identifications, the repercussions of language failure continually complicate, divide and propagate in discourse; assumptions about the validity of identifications encourage more complex ‘blind-spots’ and misrepresentations that exclude populations and have violent potentials. The more complex the layering of association between identifications becomes, the more obscured their foundational failure, their nature of non-innateness, is, faced as truth, affective as justice. The ‘affective’ foundation behind these powerful associations and assumptions is myth. Origin myth, or a narrative that explains the beginning of some worldly phenomena, founds, and adapts to the needs of culture and society. Teaching students to regard risks of language use as being foundational in their cultural thought, their criticism, and their communication, enriches their capability to negotiate and participate ambivalently in conflicts faced during their daily lives.

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2021