Hundreds of thousands of people die annually from malaria; a protozoan of the genus Plasmodium is responsible for this mortality. The Plasmodium parasite undergoes several life stages within the mosquito vector, the transition between which require passage across the lumen of the mosquito midgut. It has been observed that in about 15% of parasites that develop ookinetes in the mosquito abdomen, sporozoites never develop in the salivary glands, indicating that passage across the midgut lumen is a significant barrier in parasite development (Gamage-Mendis et al., 1993). We aim to investigate a possible correlation between passage through the midgut lumen and drug-resistance trends in Plasmodium falciparum parasites. This study contains a total of 1024 Anopheles mosquitoes: 187 Anopheles gambiae and 837 Anopheles funestus samples collected in high malaria transmission areas of Mozambique between March and June of 2016. Sanger sequencing will be used to determine the prevalence of known resistance alleles for anti-malarial drugs: chloroquine resistance transporter (pfcrt), multidrug resistance (pfmdr1) gene, dihydropteroate synthase (pfdhps) and dihydrofolate reductase (pfdhfr). We compare prevalence of resistance between abdomen and head/thorax in order to determine whether drug resistant parasites are disproportionately hindered during their passage through the midgut lumen. A statistically significant difference between resistance alleles in the two studied body sections supports the efficacy of new anti-malarial gene surveillance strategies in areas of high malaria transmission.

Activist burnout theory has produced minimal but meaningful literature and research that explores the dynamics of burnout culture, movement in-fighting, marginalized identities, and dimensions of burnout symptoms. Black feminist visionaries and writers such as Audre Lorde and bell hooks have developed theories of love, self-care and community as central to resistance that have informed my research approach. Thus, my study aims to investigate activist burnout from a perspective that marries popular activist burnout theory with these frameworks of self-care and community. I conducted a survey of Arizona State University student organizers and activists (N=34) to address the following research questions: What are the causes and symptoms of burnout for Arizona State University activists and organizers? How have self-care and community played a role in their work and countered burnout? Can working conceptions of self-care and community serve as resistance in ways that feel meaningful to activists? The survey was broken into three dimensions: “Demographics and Experience,” “Burnout,” and “Self-Care and Community.” The results reinforced prior findings on established toxic cultures and burnout symptoms but introduced complications to working theories, such as the connections between cycles of burnout and the cyclical nature of electoral politics along with the roles of chronic and mental illness. Respondents largely demonstrated conceptions of self-care and community as resistance but also demonstrated personal and professional barriers to putting these conceptions into practice.