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- Genre: Academic theses
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This study aims to unearth monological and monocultural discourses buried under the power of the dominant biomedical model governing the HIV/AIDS debate. The study responds to an apparent consensus, rooted in Western biomedicine and its "standardizations of knowledge," in the production of the current HIV/AIDS discourse, especially in Sub-Saharan Africa. As a result, biomedicine has become the dominant actor (in) writing and rewriting discourse for the masses while marginalizing other forms of medical knowledge. Specifically, in its development, the Western biomedical model has arguably isolated the disease from its human host and the social experiences that facilitate the disease's transmission, placing it in the realm of laboratories and scientific experts and giving full ownership to Western medical discourse. Coupled with Western assumptions about African culture that reproduce a one-sided discourse informing the social construction of HIV/AIDS in Africa, this Western monopoly thus constrained the extent and efficacy of international prevention efforts. In this context, the goal for this study is not to demonize the West and biomedicine in general. Rather, this study seeks an alternative and less monolithic understanding currently absent in scientific discourses of HIV/AIDS that frequently elevates Western biomedicine over indigenous medicine; the Western expert over the local. The study takes into account the local voices of Sub-Saharan Africa and how the system has affected them, this study utilizes a Foucauldian approach to analyze discourse as a way to explore how certain ways of knowledge are formed in relation to power. This study also examines how certain knowlege is maintaned and reinforced within specific discourses.
ContributorsAbdalla, Mohamed (Author) / Jacobs, Bertram (Thesis advisor) / Robert, Jason (Committee member) / Klimek, Barbara (Committee member) / Arizona State University (Publisher)
Created2014
Description
Natural hydrogenases catalyze the reduction of protons to molecular hydrogen reversibly under mild conditions; these enzymes have an unusual active site architecture, in which a diiron site is connected to a cubane type [4Fe-4S] cluster. Due to the relevance of this reaction to energy production, and in particular to sustainable fuel production, there have been substantial amount of research focused on developing biomimetic organometallic models. However, most of these organometallic complexes cannot revisit the structural and functional fine-tuning provided by the protein matrix as seen in the natural enzyme. The goal of this thesis is to build a protein based functional mimic of [Fe-Fe] hydrogenases. I used a 'retrosynthetic' approach that separates out two functional aspects of the natural enzyme. First, I built an artificial electron transfer domain by engineering two [4Fe-4S] cluster binding sites into an existing protein, DSD, which is a de novo designed domain swapped dimer. The resulting protein, DSD-bis[4Fe-4S], contains two clusters at a distance of 36 Å . I then varied distance between two clusters using vertical translation along the axis of the coiled coil; the resulting protein demonstrates efficient electron transfer to/from redox sites. Second, I built simple, functional artificial hydrogenases by using an artificial amino acid comprising a 1,3 dithiol moiety to anchor a biomimetic [Fe-Fe] active site within the protein scaffold Correct incorporation of the cluster into a model helical peptide was verified by UV-Vis, FTIR, ESI-MS and CD spectroscopy. This synthetic strategy is extended to the de novo design of more complex protein architectures, four-helix bundles that host the di-iron cluster within the hydrophobic core. In a separate approach, I developed a generalizable strategy to introduce organometallic catalytic sites into a protein scaffold. I introduced a biomimetic organometallic complex for proton reduction by covalent conjugation to biotin. The streptavidin-bound complex is significantly more efficient in photocatalytic hydrogen production than the catalyst alone. With these artificial proteins, it will be possible to explore the effect of second sphere interactions on the activity of the diiron center, and to include in the design properties such as compatibility with conductive materials and electrodes.
ContributorsRoy, Anindya (Author) / Ghirlanda, Giovanna (Thesis advisor) / Yan, Hao (Committee member) / Gust, Devens (Committee member) / Arizona State University (Publisher)
Created2014
Description
Regional differences of inventive activity and economic growth are important in economic geography. These differences are generally explained by the theory of localized knowledge spillovers, which argues that geographical proximity among economic actors fosters invention and innovation. However, knowledge production involves an increasing number of actors connecting to non-local partners. The space of knowledge flows is not tightly bounded in a given territory, but functions as a network-based system where knowledge flows circulate around alignments of actors in different and distant places. The purpose of this dissertation is to understand the dynamics of network aspects of knowledge flows in American biotechnology. The first research task assesses both spatial and network-based dependencies of biotechnology co-invention across 150 large U.S. metropolitan areas over four decades (1979, 1989, 1999, and 2009). An integrated methodology including both spatial and social network analyses are explicitly applied and compared. Results show that the network-based proximity better defines the U.S. biotechnology co-invention urban system in recent years. Co-patenting relationships of major biotechnology centers has demonstrated national and regional association since the 1990s. Associations retain features of spatial proximity especially in some Midwestern and Northeastern cities, but these are no longer the strongest features affecting co-inventive links. The second research task examines how biotechnology knowledge flows circulate over space by focusing on the structural properties of intermetropolitan co-invention networks. All analyses in this task are conducted using social network analysis. Evidence shows that the architecture of the U.S. co-invention networks reveals a trend toward more organized structures and less fragmentation over the four years of analysis. Metropolitan areas are increasingly interconnected into a large web of networked environment. Knowledge flows are less likely to be controlled by a small number of intermediaries. San Francisco, New York, Boston, and San Diego monopolize the central positions of the intermetropolitan co-invention network as major American biotechnology concentrations. The overall network-based system comes close to a relational core/periphery structure where core metropolitan areas are strongly connected to one another and to some peripheral areas. Peripheral metropolitan areas are loosely connected or even disconnected with each other. This dissertation provides empirical evidence to support the argument that technological collaboration reveals a network-based system associated with different or even distant geographical places, which is somewhat different from the conventional theory of localized knowledge spillovers that once dominated understanding of the role of geography in technological advance.
ContributorsLee, Der-Shiuan (Author) / Ó Huallacháin, Breandán (Thesis advisor) / Anselin, Luc (Committee member) / Kuby, Michael (Committee member) / Lobo, Jose (Committee member) / Arizona State University (Publisher)
Created2011
Description本文研究了产品和研发构成如何影响制药业被收购生物科技公司的并购溢价。研究结果如下。首先，拥有更多产品的目标公司获得更高的溢价。具体来说，这种影响来源于创新药物的数量。第二，目标公司的研发管线的数量与溢价没有明显的相关性。这一结果与强调了不同阶段研发管线携带的公共信息、风险和回报的文献相符。我的实证证据表明，II期管线（III期管线）的数量正向（负向）预测并购溢价。第三，创新产品和II期管线数量的影响部分地被制药业格局特有的协同效应所解释。此外，改良药数量也通过提高协同效应来创造溢价。本文结果对从业者具有参考价值。上述结论来自2010年至2021年的纳斯达克样本，对仍处于早期阶段的中国生物科技产业来说，可以作为一个重要的参考。
ContributorsGuo, Xiaobin (Author) / Zhu, David (Thesis advisor) / Chang, Chun (Thesis advisor) / Jiang, Zhan (Committee member) / Arizona State University (Publisher)
Created2023
Description
This dissertation investigates the relationship between the universal aspirationsof technology and the particularity of place, by way of close participant observation with biotechnology companies in the San Francisco Bay Area. Its central claim is that the aspiration to placelessness in the development of science and technology operates as material configurations, modes of subjectivation, and historical conditions particular to places. Following Foucault’s late work in ethics, I conduct a series of sustained investigations into the reflective modes of critique biotechnologists make in thinking of and being in the San Francisco Bay Area. I show the ways the aspiration to placelessness exists in place at four different vantage points: the organization, the city, the broader cultural history of the region, and the practices of self-cultivation undertaken by technologists. Within biotechnology organizations, biological work is digitized and automated only through an intensification of bespoke material infrastructures, physical labor, and tacit institutional knowledge. Biotechnology organizations have come into existence through a history of settler colonial erasure, industrial devastation, post-war industrial decay, and urban renewal in Bay Area industrial suburbs and neighborhoods. A nostalgic imagination of the broader San Francisco Bay Area and its history of counterculture become mobilized as an antidote to the felt lifelessness of these forms of urban renewal and technological order and incorporated back into engineering practice. Finally, the technologist themselves must aspire to placelessness, in ways critiqued by local landless people’s movements who offer an alternative ethic to place in their imperative to gentrifiers to “move home with your parents.” I conclude by reflecting on the ways interlocutors at each of these vantage points are actively exploring the creation of more enduring relationships to place in the face of the unintended but intensified forms of social suffering in zones of technological innovation.
ContributorsHammang, Anne (Author) / Bennett, Gaymon (Thesis advisor) / Hurlbut, J. Benjamin (Committee member) / Frow, Emma (Committee member) / Arizona State University (Publisher)
Created2022
Description
This dissertation explores the contemporary politics of global transformation: the ways biological expertise and economic rationalities are positioned as agents of governance in the face of emerging global crisis. It examines visions for a new bioeconomy that are offered in response to impending global crisis. Leaders point to calculations of global population growth and resource depletion to predict future crises and call for a new bioeconomy as a pillar of sustainable and “good” governance.
Focusing on visions and practices of bioeconomy-making in the U.S. and Brazil, the dissertation examines bioeconomy discourse as a response to global crisis and a framework of global governance that promises resource abundance and human wellbeing. Bioeconomy discourse makes visible shared notions of how the world is and how it should be that animate the world-making practices of bioeconomy. The dissertation analyzes the bioeconomy as simultaneously a product of existing institutional and nationally situated values and rationalities, and a significant site of performative novelty. It is an effort to reformulate existing projects in the biosciences—from technology regulation to market formation—and establish new rationalities of governance in the name of producing thoroughgoing transformations to both the global economy and to life itself.
Framing existing scientific and economic rationalities as suppressed and misdirected in their power to govern, bioeconomy proponents envision a novel order derivable from the proper conjugation of biological and economic rationalities. Through the lens of bioconstitutionalism, the dissertation elucidates how national, scientific and public rights and responsibilities are coproduced in relation to a sociotechnical imaginary of vital conjuring. Underwritten by the imaginary of vital conjuring, visions of a future transformed promise that abundance and order can be called up from a tangle of crisis and decay. The imaginary of vital conjuring marries a vision of the technological potential of biological life and the forms of economy capable of unlocking that potential. This vision of bioeconomy, the dissertation argues, is a vision of governance: of the right relationships between state, citizen and science.
ContributorsDoezema, Tess A (Author) / Hurlbut, James B (Thesis advisor) / Miller, Clark A. (Committee member) / Bennett, Gaymon (Committee member) / Arizona State University (Publisher)
Created2020
Description
Over the past four decades, DNA nanotechnology has grown exponentially from a field focused on simple structures to one capable of synthesizing complex nano-machines capable of drug delivery, nano-robotics, digital data storage, logic gated circuitry, nano-photonics, and other applications. The construction of these nanostructures is possible because of the predictable and programmable Watson-Crick base pairing of DNA. However, there is an increasing need for the incorporation of chemical diversity and functionality into these nanostructures. To overcome this challenge, this work explored creating hybrid DNA nanostructures by making self-assembling small molecule/protein-DNA conjugates.In one direction, well studied host-guest interactions (i.e. cucurbituril[7]-adamantane) were used as the choice of self-assembling species. Binding studies using these small molecule-DNA conjugates were performed and thereafter they were used to assemble larger DNA origami nanostructures. Finally, a stimulus responsive DNA nano-box that opens and closes based on these interactions was also demonstrated. In another direction, a trimeric KDPG aldolase protein-DNA conjugate was probed as a structural building block by assembling it into a DNA origami tetrahedron with four cavities. This hybrid building block was thereafter characterized by single particle cryo-EM and the resulting electron density map was best fit by simulating origami cages with varying number of proteins (ranging from 0 to 4).
Next, to increase access and for larger democratization of the field, an automation designer software tool capable of making DNA nanostructures was made. In this work, the focus was on making curved 3D DNA nanostructures. The last direction probed in this work was to make optical metamaterials based on complex 3D DNA architectures. Realization of a self-assembled 3D tetrastack geometry is still an unachieved dream in the field of DNA self-assembly. Thus, this direction was probed using DNA origami icosahedrons. Finally, the work covered in my thesis probes multiple directions for advancing DNA nanotechnology, both fundamentally and for potential applications.
ContributorsNarayanan Pradeep, Raghu (Author) / Yan, Hao HY (Thesis advisor) / Stephanopoulos, Nicholas NS (Thesis advisor) / Liu, Yan YL (Committee member) / Mills, Jeremy JHM (Committee member) / Arizona State University (Publisher)
Created2021