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Description
Honey bees (Apis mellifera) are responsible for pollinating nearly 80\% of all pollinated plants, meaning humans depend on honey bees to pollinate many staple crops. The success or failure of a colony is vital to global food production. There are various complex factors that can contribute to a colony's failure,

Honey bees (Apis mellifera) are responsible for pollinating nearly 80\% of all pollinated plants, meaning humans depend on honey bees to pollinate many staple crops. The success or failure of a colony is vital to global food production. There are various complex factors that can contribute to a colony's failure, including pesticides. Neonicotoids are a popular pesticide that have been used in recent times. In this study we concern ourselves with pesticides and its impact on honey bee colonies. Previous investigations that we draw significant inspiration from include Khoury et Al's \emph{A Quantitative Model of Honey Bee Colony Population Dynamics}, Henry et Al's \emph{A Common Pesticide Decreases Foraging Success and Survival in Honey Bees}, and Brown's \emph{ Mathematical Models of Honey Bee Populations: Rapid Population Decline}. In this project we extend a mathematical model to investigate the impact of pesticides on a honey bee colony, with birth rates and death rates being dependent on pesticides, and we see how these death rates influence the growth of a colony. Our studies have found an equilibrium point that depends on pesticides. Trace amounts of pesticide are detrimental as they not only affect death rates, but birth rates as well.
ContributorsSalinas, Armando (Author) / Vaz, Paul (Thesis director) / Jones, Donald (Committee member) / School of Mathematical and Statistical Sciences (Contributor) / School of International Letters and Cultures (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
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Description
The giant green sea anemone, Anthopleura xanthogrammica, hosts two different endosymbiotic algae. One is a unicellular chlorophyte, Elliptochloris marina; the other is Symbiodinium muscatinei, a dinoflagellate. Hosting these different symbionts influences the life history strategy of A. xanthogrammica's congener A. elegantissima, directly impacting its reproductive strategy (asexual vs. sexual). My

The giant green sea anemone, Anthopleura xanthogrammica, hosts two different endosymbiotic algae. One is a unicellular chlorophyte, Elliptochloris marina; the other is Symbiodinium muscatinei, a dinoflagellate. Hosting these different symbionts influences the life history strategy of A. xanthogrammica's congener A. elegantissima, directly impacting its reproductive strategy (asexual vs. sexual). My study sought to examine whether the type and density of symbiont also affects the reproductive condition of A. xanthogrammica, which reproduces only sexually. Gonad development was measured in anemones from Slip Point, Clallam Bay, WA and Tongue Point, WA along with symbiont type and density per mg of anemone protein. The results indicate a trend towards brown anemones having more developed gonads, especially in males. This may mean that A. xanthogrammica anemones that host zooxanthellae are more reproductively fit than zoochlorellate anemones. Thus, it may be favorable for anemones to host zooxanthellae. This is especially true in summer months when the high temperatures and mid-day low tides coincide with the period of most rapid gonad development.
ContributorsGasbarro, Ryan Patrick (Author) / Neuer, Susanne (Thesis director) / Rutowski, Ronald (Committee member) / Bingham, Brian (Committee member) / Barrett, The Honors College (Contributor) / School of Earth and Space Exploration (Contributor) / School of Life Sciences (Contributor)
Created2015-05
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Description
For colonies of ponerine ant species, sterility regulation after a founding queen's death is not totally achieved in the worker caste, and the possibility of sexual reproduction is opened to workers. The persisting survival of these colonies is dependent on capturing the optimal reproductive ratio; yet, an informational gap bounds

For colonies of ponerine ant species, sterility regulation after a founding queen's death is not totally achieved in the worker caste, and the possibility of sexual reproduction is opened to workers. The persisting survival of these colonies is dependent on capturing the optimal reproductive ratio; yet, an informational gap bounds the mechanisms detailing the selection of new reproductives and the suppression of ovarian development in rejected reproductives. We investigated the mechanisms of worker policing, one of the primary methods of ovarian suppression, through continuous video observation for a period of five days at the start of colony instability. Observations suggest policing in H. saltator is performed by a majority of a colony, including potential reproductives, and requires multiple events to fully discourage ovarian growth.
ContributorsChien, Jeffrey (Co-author) / Barat Ali, Fatima (Co-author) / Kang, Yun (Thesis director) / Liebig, Juergen (Committee member) / School of Mathematical and Statistical Sciences (Contributor) / Mechanical and Aerospace Engineering Program (Contributor) / Barrett, The Honors College (Contributor)
Created2018-12
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Description
Lactase persistence is the ability of adults to digest lactose in milk (Segurel & Bon, 2017). Mammals are generally distinguished by their mammary glands which gives females the ability to produce milk and feed their newborn children. The new born therefore requires the ability to breakdown the lactose in the

Lactase persistence is the ability of adults to digest lactose in milk (Segurel & Bon, 2017). Mammals are generally distinguished by their mammary glands which gives females the ability to produce milk and feed their newborn children. The new born therefore requires the ability to breakdown the lactose in the milk to ensure its proper digestion (Segurel & Bon, 2017). Generally, humans lose the expression of lactase after weaning, which prevents them being able to breakdown lactose from dairy (Flatz, 1987).
My research is focused on the people of Turkana, a human pastoral population inhabiting Northwest Kenya. The people of Turkana are Nilotic people that are native to the Turkana district. There are currently no conclusive studies done on evidence for genetic lactase persistence in Turkana. Therefore, my research will be on the evolution of lactase persistence in the people of Turkana. The goal of this project is to investigate the evolutionary history of two genes with known involvement in lactase persistence, LCT and MCM6, in the Turkana. Variants in these genes have previously been identified to result in the ability to digest lactose post-weaning age. Furthermore, an additional study found that a closely related population to the Turkana, the Massai, showed stronger signals of recent selection for lactase persistence than Europeans in these genes. My goal is to characterize known variants associated with lactase persistence by calculating their allele frequencies in the Turkana and conduct selection scans to determine if LCT/MCM6 show signatures of positive selection. In doing this, we conducted a pilot study consisting of 10 female Turkana individuals and 10 females from four different populations from the 1000 genomes project namely: the Yoruba in Ibadan, Nigeria (YRI); Luhya in Webuye, Kenya; Utah Residents with Northern and Western European Ancestry (CEU); and the Southern Han Chinese. The allele frequency calculation suggested that the CEU (Utah Residents with Northern and Western European Ancestry) population had a higher lactase persistence associated allele frequency than all the other populations analyzed here, including the Turkana population. Our Tajima’s D calculations and analysis suggested that both the Turkana population and the four haplotype map populations shows signatures of positive selection in the same region. The iHS selection scans we conducted to detect signatures of positive selection on all five populations showed that the Southern Han Chinese (CHS), the LWK (Luhya in Webuye, Kenya) and the YRI (Yoruba in Ibadan, Nigeria) populations had stronger signatures of positive selection than the Turkana population. The LWK (Luhya in Webuye, Kenya) and the YRI (Yoruba in Ibadan, Nigeria) populations showed the strongest signatures of positive selection in this region. This project serves as a first step in the investigation of lactase persistence in the Turkana population and its evolution over time.
ContributorsJobe, Ndey Bassin (Author) / Wilson Sayres, Melissa (Thesis director) / Paaijmans, Krijn (Committee member) / Taravella, Angela (Committee member) / School of Earth and Space Exploration (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
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Description
Division of Labor among social insects is frequently discussed in regards to the colony's worker population. However, before a colony achieves a worker population, a queen is required to perform all of the tasks necessary for her survival: foraging, building the colony, and brood care. A simple ODE model was

Division of Labor among social insects is frequently discussed in regards to the colony's worker population. However, before a colony achieves a worker population, a queen is required to perform all of the tasks necessary for her survival: foraging, building the colony, and brood care. A simple ODE model was developed through the use of a framework of replicator equations in dynamical environments to investigate how queen ants perform and distribute all of the tasks necessary for her and her colony's survival by incorporating individual internal thresholds and environmental stimulus. Modi�cations to the internal threshold, risk of performing the task, and the rate of increase of the environmental stimulus were also explored. Because of the simplicity of the model, it could also be used to measure the task performance of larger populations of social insects. However, the model has only been applied to the data collected from Pogonomyrmex barbatus single queen ants.
ContributorsKincade, Katherine Margaret (Author) / Kang, Yun (Thesis director) / Fewell, Jennifer (Committee member) / Lanchier, Nicolas (Committee member) / School of Mathematical and Statistical Sciences (Contributor) / Computer Science and Engineering Program (Contributor) / Barrett, The Honors College (Contributor)
Created2016-12
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Description
One hypothesis for why insects are smaller than vertebrates is that the blind-ended tracheal respiratory system challenges oxygen delivery for larger insects. Supporting this hypothesis, several studies have documented that larger insect species have larger gas transport structures than expected by isometric scaling. To further test this hypothesis, we performed

One hypothesis for why insects are smaller than vertebrates is that the blind-ended tracheal respiratory system challenges oxygen delivery for larger insects. Supporting this hypothesis, several studies have documented that larger insect species have larger gas transport structures than expected by isometric scaling. To further test this hypothesis, we performed the first inter-specific study of the scaling of spiracle size, using ten scarab beetle species, including some of the most massive insects. Using micro-CT, we measured the cross sectional area and depth of all eight spiracles. Areas of large spiracles in the anterior portion of the animal showed hypermetric scaling, varying approximately with mass^0.8. However, because diffusive capacities scaled with lower slopes than metabolic rates, larger beetles had a 10-fold higher required PO2 gradient across the spiracles to sustain oxygen consumption by diffusion. Despite this trend, calculations suggest that large beetles can exchange oxygen by diffusion across the spiracles at rest, but likely no beetles can do so during flight. Advective capacities through the spiracles scale with mass^1.8, suggestive of a switch toward greater use of convection and/or reduced required pressures in larger beetles.
ContributorsWagner, Julian Morgan (Author) / Harrison, Jon F. (Thesis director) / VandenBrooks, John (Committee member) / Miller, Laura (Committee member) / School of Mathematical and Statistical Sciences (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2017-05
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Description

The Star Planet Activity Research CubeSat (SPARCS) will be a 6U CubeSat devoted to photometric monitoring of M dwarfs in the far-ultraviolet (FUV) and near-ultraviolet (NUV) (160 and 280 nm respectively), measuring the time-dependent spectral slope, intensity and evolution of M dwarf stellar UV radiation. The delta-doped detectors baselined for

The Star Planet Activity Research CubeSat (SPARCS) will be a 6U CubeSat devoted to photometric monitoring of M dwarfs in the far-ultraviolet (FUV) and near-ultraviolet (NUV) (160 and 280 nm respectively), measuring the time-dependent spectral slope, intensity and evolution of M dwarf stellar UV radiation. The delta-doped detectors baselined for SPARCS have demonstrated more than five times the in-band quantum efficiency of the detectors of GALEX. Given that red:UV photon emission from cool, low-mass stars can be million:one, UV observation of thes stars are susceptible to red light contamination. In addition to the high efficiency delta-doped detectors, SPARCS will include red-rejection filters to help minimize red leak. Even so, careful red-rejection and photometric calibration is needed. As was done for GALEX, white dwarfs are used for photometric calibration in the UV. We find that the use of white dwarfs to calibrate the observations of red stars leads to significant errors in the reported flux, due to the differences in white dwarf and red dwarf spectra. Here we discuss the planned SPARCS calibration model and the color correction, and demonstrate the importance of this correction when recording UV measurements of M stars taken by SPARCS.

ContributorsOsby, Ella (Author) / Shkolnik, Evgenya (Thesis director) / Ardila, David (Committee member) / School of Earth and Space Exploration (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
Description
The cerebellum is recognized for its role in motor movement, balance, and more recently, social behavior. Cerebellar injury at birth and during critical periods reduces social preference in animal models and increases the risk of autism in humans. Social behavior is commonly assessed with the three-chamber test, where a mouse

The cerebellum is recognized for its role in motor movement, balance, and more recently, social behavior. Cerebellar injury at birth and during critical periods reduces social preference in animal models and increases the risk of autism in humans. Social behavior is commonly assessed with the three-chamber test, where a mouse travels between chambers that contain a conspecific and an object confined under a wire cup. However, this test is unable to quantify interactive behaviors between pairs of mice, which could not be tracked until the recent development of machine learning programs that track animal behavior. In this study, both the three-chamber test and a novel freely-moving social interaction test assessed social behavior in untreated male and female mice, as well as in male mice injected with hM3Dq (excitatory) DREADDs. In the three-chamber test, significant differences were found in the time spent (female: p < 0.05, male: p < 0.001) and distance traveled (female: p < 0.05, male: p < 0.001) in the chamber with the familiar conspecific, compared to the chamber with the object, for untreated male, untreated female, and mice with activated hM3Dq DREADDs. A social memory test was added, where the object was replaced with a novel mouse. Untreated male mice spent significantly more time (p < 0.05) and traveled a greater distance (p < 0.05) in the chamber with the novel mouse, while male mice with activated hM3Dq DREADDs spent more time (p<0.05) in the chamber with the familiar conspecific. Data from the freely-moving social interaction test was used to calculate freely-moving interactive behaviors between pairs of mice and interactions with an object. No sex differences were found, but mice with excited hM3Dq DREADDs engaged in significantly more anogenital sniffing (p < 0.05) and side-side contact (p < 0.05) behaviors. All these results indicate how machine learning allows for nuanced insights into how both sex and chemogenetic excitation impact social behavior in freely-moving mice.
ContributorsNelson, Megan (Author) / Verpeut, Jessica (Thesis director) / Bimonte-Nelson, Heather (Committee member) / Barrett, The Honors College (Contributor) / Department of Psychology (Contributor) / School of Life Sciences (Contributor) / School of Mathematical and Statistical Sciences (Contributor)
Created2024-05
Description
Gamma-ray bursts (GRBs) are a type of astrophysical transient resulting from the most energetic explosions known in the universe. The explosions occur in distant galaxies, and their bright initial emission may only last a few seconds. Colibri is a telescope being built at the San Pedro Martir

Gamma-ray bursts (GRBs) are a type of astrophysical transient resulting from the most energetic explosions known in the universe. The explosions occur in distant galaxies, and their bright initial emission may only last a few seconds. Colibri is a telescope being built at the San Pedro Martir Observatory in Baja, CA, MX with high sensitivity in order to study these events at a high redshift. Due to how quickly GRBs occur, it is essential to develop an image reduction pipeline that can quickly and accurately detect these events. Using existing image reduction software from Coatli, which was programmed and optimized for speed using python, numerous time trials were performed in order to determine if the pipeline meets the time requirements with various factors being adjusted. The goal of this experiment is for the telescope to respond to, capture, and reduce the images in under 3 minutes. It was determined that the reduction was optimized when the number of files to be reduced was set equal to 16 or higher by changing the batch number and the blank sky subtraction function was performed. As for the number of exposures, one can take up to four 30 second exposures or twenty 5 second exposures and reduce them in under 3 minutes.
ContributorsHeiligenstein, Wren (Author) / Butler, Nathaniel (Thesis director) / Jansen, Rolf (Committee member) / Dimitrova, Tzvetelina (Committee member) / Barrett, The Honors College (Contributor) / School of Earth and Space Exploration (Contributor)
Created2024-05
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Description
Although extracellular throughout their lifecycle, trypanosomes are able to persist despite strong host immune responses through a process known as antigenic variation involving a large, highly diverse family of surface glycopro- tein (VSG) genes, only one of which is expressed at a time. Previous studies have used mathematical models to

Although extracellular throughout their lifecycle, trypanosomes are able to persist despite strong host immune responses through a process known as antigenic variation involving a large, highly diverse family of surface glycopro- tein (VSG) genes, only one of which is expressed at a time. Previous studies have used mathematical models to investigate the relationship between VSG switching and the dynamics of trypanosome infections, but none have explored the role of multiple VSG expression sites or the contribution of mosaic gene conversion events involving VSG pseudogenes.
ContributorsKoury, Michael Andrew (Author) / Taylor, Jesse (Thesis director) / Gumel, Abba (Committee member) / Department of Physics (Contributor) / School of Mathematical and Statistical Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05