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Antibiotic Resistance in Bacteria: Public, Economic, and Medical Perspectives

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Antibiotics, bacteria, and the continuing trend of antibiotic resistance increasing in various bacteria strains is a complex and multifaceted set of relationships explored in this thesis. Examining a variety of published literature in various sectors of influence, including the social, medical, and economic divisions, this thesis examined the core factors…

Antibiotics, bacteria, and the continuing trend of antibiotic resistance increasing in various bacteria strains is a complex and multifaceted set of relationships explored in this thesis. Examining a variety of published literature in various sectors of influence, including the social, medical, and economic divisions, this thesis examined the core factors and combined them into a set of recommendations for future progress. In this way, the subject of antibiotic resistance in bacteria begins with an evaluation of the history then continued into an analysis of the economic factors, a social understanding of the subject, a medical evaluation of current procedure, and a concluding framework and general set of recommendations for future use. Ultimately, these factors require a multifaceted approach in order to combat the numerous factors and contributions to emerging antibiotic resistance in bacteria both in the United States of America and around the world.
ContributorsMurphy, Emily Ann (Author) / Chhetri, Netra (Thesis director) / Ankeny, Casey (Committee member) / Hamburg, Robert (Committee member) / Barrett, The Honors College (Contributor) / Harrington Bioengineering Program (Contributor) / School of Life Sciences (Contributor)
Created2015-05
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Targeting Traumatic Brain Injury: Using Phage Display to Create a Specific Antibody for Neurocan Chondroitin Sulfate Proteoglycan Recognition

Description
This project aims to address the current protocol regarding the diagnosis and treatment of traumatic brain injury (TBI) in medical industries around the world. Although there are various methods used to qualitatively determine if TBI has occurred to a patient, this study attempts to aid in the creation of a…

This project aims to address the current protocol regarding the diagnosis and treatment of traumatic brain injury (TBI) in medical industries around the world. Although there are various methods used to qualitatively determine if TBI has occurred to a patient, this study attempts to aid in the creation of a system for quantitative measurement of TBI and its relative magnitude. Through a method of artificial evolution/selection called phage display, an antibody that binds highly specifically to a post-TBI upregulated brain chondroitin sulfate proteoglycan called neurocan has been identified. As TG1 Escheria Coli bacteria were infected with KM13 helper phage and M13 filamentous phage in conjunction, monovalent display of antibody fragments (ScFv) was performed. The ScFv bind directly to the neurocan and from screening, phage that produced ScFv's with higher affinity and specificity to neurocan were separated and purified. Future research aims to improve the ScFv characteristics through increased screening toward neurocan. The identification of a highly specific antibody could lead to improved targeting of neurocan post-TBI in-vivo, aiding researchers in quantitatively defining TBI by visualizing its magnitude.
ContributorsSeelig, Timothy Scott (Author) / Stabenfeldt, Sarah (Thesis director) / Ankeny, Casey (Committee member) / Barrett, The Honors College (Contributor) / Harrington Bioengineering Program (Contributor)
Created2015-05