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Analysis of Inhibition of Influenza Replication via Synthetic Antibodies

Description
The influenza virus, also known as "the flu", is an infectious disease that has constantly affected the health of humanity. There is currently no known cure for Influenza. The Center for Innovations in Medicine at the Biodesign Institute located on campus at Arizona State University has been developing synbodies as…

The influenza virus, also known as "the flu", is an infectious disease that has constantly affected the health of humanity. There is currently no known cure for Influenza. The Center for Innovations in Medicine at the Biodesign Institute located on campus at Arizona State University has been developing synbodies as a possible Influenza therapeutic. Specifically, at CIM, we have attempted to design these initial synbodies to target the entire Influenza virus and preliminary data leads us to believe that these synbodies target Nucleoprotein (NP). Given that the synbody targets NP, the penetration of cells via synbody should also occur. Then by Western Blot analysis we evaluated for the diminution of NP level in treated cells versus untreated cells. The focus of my honors thesis is to explore how synthetic antibodies can potentially inhibit replication of the Influenza (H1N1) A/Puerto Rico/8/34 strain so that a therapeutic can be developed. A high affinity synbody for Influenza can be utilized to test for inhibition of Influenza as shown by preliminary data. The 5-5-3819 synthetic antibody's internalization in live cells was visualized with Madin-Darby Kidney Cells under a Confocal Microscope. Then by Western Blot analysis we evaluated for the diminution of NP level in treated cells versus untreated cells. Expression of NP over 8 hours time was analyzed via Western Blot Analysis, which showed NP accumulation was retarded in synbody treated cells. The data obtained from my honors thesis and preliminary data provided suggest that the synthetic antibody penetrates live cells and targets NP. The results of my thesis presents valuable information that can be utilized by other researchers so that future experiments can be performed, eventually leading to the creation of a more effective therapeutic for influenza.
ContributorsHayden, Joel James (Author) / Diehnelt, Chris (Thesis director) / Johnston, Stephen (Committee member) / Legutki, Bart (Committee member) / Barrett, The Honors College (Contributor) / Department of Psychology (Contributor) / Department of Chemistry and Biochemistry (Contributor)
Created2014-05
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Teaching Doctors to Respond with Empathy: A Pilot Study

Description
Empathy is a critical component of high-quality healthcare. When present in the clinical encounter, empathy is important to physicians (empathy is correlated with reductions in physician anxiety and burnout) and to patients (empathy is correlated with better medical decision making, enhanced trust, and improved treatment adherence). Unfortunately, there is an…

Empathy is a critical component of high-quality healthcare. When present in the clinical encounter, empathy is important to physicians (empathy is correlated with reductions in physician anxiety and burnout) and to patients (empathy is correlated with better medical decision making, enhanced trust, and improved treatment adherence). Unfortunately, there is an empathy gap in healthcare–physicians often miss opportunities to demonstrate empathy to their patients. This leaves patients feeling unheard, less likely to bring up details important to their care, and less likely to follow treatment guidelines from physicians, thus disrupting the physician-patient relationship. Luckily, communicating with empathy is a skill that can be taught and learned. With the right tools, learners can strengthen their empathic muscle and become better prepared for responding in difficult situations. The present thesis aims to validate a new tool for teaching empathy to medical trainees. This tool, an empathic communication guide, is drawn from social work as well as medical expertise. It is catered specifically to how medical trainees are accustomed to learning and provides the actual words to say in order to respond with empathy in difficult situations. A group of 8 palliative care fellows at MD Anderson Cancer Center in Houston, Texas received a copy of this guide and participated in an accompanying communication workshop. To gauge empathic responding ability, fellows completed pre- and post- surveys and patient simulations. These data were analyzed using a combination of novel and established methods for quantifying empathic behaviors. Fellows’ empathic communication skill significantly improved after exposure to the guide opening avenues for future study and application.
ContributorsMeyer, Laura Grace (Author) / Shafer, Michael S (Thesis advisor) / Epner, Daniel E (Committee member) / Beyers, Michelle (Committee member) / Arizona State University (Publisher)
Created2020

Empathic Communication in Case Investigation: A Responding Guide for Difficult Situations

Description

Being prepared to respond to difficult situations that arise in public health practice is an essential skill for the public health workforce.This empathic responding guide was designed to train students, volunteers, and staff of the ASU COVID-19 Case Investigation Team. The guide provides an overview of empathic communication, walks through…

Being prepared to respond to difficult situations that arise in public health practice is an essential skill for the public health workforce.This empathic responding guide was designed to train students, volunteers, and staff of the ASU COVID-19 Case Investigation Team. The guide provides an overview of empathic communication, walks through a framework for responding with empathy, and outlines common difficult situations that arise in public health along with ways to respond with empathy to these situations. This guide can be adapted to a wide variety of settings and is meant to be used as a training tool for public health case investigators and other staff. This guide, available in a full and an abridged version, can be paired with hands-on workshops to provide engaging continuing education opportunities for public health teams.
ContributorsMeyer, Laura Grace (Author) / Arizona State University. AZ Infectious Disease Epi (AIDE) Lab (Contributor) / Arizona State University. Public Health Program (Contributor)
Created2021-07-12
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Empathic Communication in Public Health: A Responding Guide for Difficult Situations [Abridged]

Description

This communication guide outlines examples of specific situations that are difficult to respond to, and pairs them with examples of how to respond with empathy. This guide depicts these difficult case statements as rows with bold, italic text. Beneath each scenario is an example of an empathic response (underlined) that…

This communication guide outlines examples of specific situations that are difficult to respond to, and pairs them with examples of how to respond with empathy. This guide depicts these difficult case statements as rows with bold, italic text. Beneath each scenario is an example of an empathic response (underlined) that can lead to a factual response or survey prompt (Figure 1). The responses use empathic communication to show the case that you are witnessing the emotion, rather than moving to the survey without acknowledging emotion. There is no one right answer to any difficult case statement.
ContributorsMeyer, Laura Grace (Author) / Arizona State University. Public Health Program (Contributor) / Arizona State University. AZ Infectious Disease Epi (AIDE) Lab (Contributor)
Created2021-07-12
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Empathic Communication in Case Investigation: A Responding Guide for Difficult Situations [Full guide]

Description

Being prepared to respond to difficult situations that arise in public health practice is an essential skill for the public health workforce.This empathic responding guide was designed to train students, volunteers, and staff of the ASU COVID-19 Case Investigation Team. The guide provides an overview of empathic communication, walks through…

Being prepared to respond to difficult situations that arise in public health practice is an essential skill for the public health workforce.This empathic responding guide was designed to train students, volunteers, and staff of the ASU COVID-19 Case Investigation Team. The guide provides an overview of empathic communication, walks through a framework for responding with empathy, and outlines common difficult situations that arise in public health along with ways to respond with empathy to these situations. This guide can be adapted to a wide variety of settings and is meant to be used as a training tool for public health case investigators and other staff. This guide can be paired with hands-on workshops to provide engaging continuing education opportunities for public health teams.
ContributorsMeyer, Laura Grace (Author) / Arizona State University. Public Health Program (Contributor) / Arizona State University. AZ Infectious Disease Epi (AIDE) Lab (Contributor)
Created2021-07-12