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A point of care glucose sensor using electrochemical impedance spectroscopy (EIS) with a glutaraldehyde-linked enzyme shows promise as an effective biosensor platform. This report details the characterization of various factors on optimal binding frequency (OBF) and sensor performance to better prepare the sensor for future experimentation. Utilizing a screen printed

A point of care glucose sensor using electrochemical impedance spectroscopy (EIS) with a glutaraldehyde-linked enzyme shows promise as an effective biosensor platform. This report details the characterization of various factors on optimal binding frequency (OBF) and sensor performance to better prepare the sensor for future experimentation. Utilizing a screen printed carbon electrode, the necessary amount of glucose oxidase was determined to be 10 mg/mL. Binding time trials ranging from 1-3 minutes demonstrated that 1.5 minutes was the optimal binding time. This timeframe produced the strongest impedance response at each glucose concentration. Using this enzyme concentration and binding time, the native OBF of the biosensor was found to be 1.18 Hz using vector analysis. Temperature testing showed little change in OBF in sensors exposed to 4 \u00B0C through 43.3 \u00B0C. Only exposure to 60 \u00B0C resulted in rapid OBF change which was likely due to glucose oxidase becoming denatured. Humidity tests showed little change in OBF and sensor performance between sensors prepared at the humidities of 7.5%, 10.625% and 16.5% humidity. Alternatively, solutions containing common interference molecules such as uric acid, acetaminophen, and ascorbic acid resulted in a highly shifted OBF and drastically reduced signal.
ContributorsMatloff, Daniel (Co-author) / Khanwalker, Mukund (Co-author) / Johns, Jared (Co-author) / LaBelle, Jeffrey (Thesis director) / Pizziconi, Vincent (Committee member) / Lin, Chi (Committee member) / Dean, W.P. Carey School of Business (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2018-12
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Description

2D fetal echocardiography (ECHO) can be used for monitoring heart development in utero. This study’s purpose is to empirically model normal fetal heart growth and function changes during development by ECHO and compare these to fetuses diagnosed with and without cardiomyopathy with diabetic mothers. There are existing mathematical models describing

2D fetal echocardiography (ECHO) can be used for monitoring heart development in utero. This study’s purpose is to empirically model normal fetal heart growth and function changes during development by ECHO and compare these to fetuses diagnosed with and without cardiomyopathy with diabetic mothers. There are existing mathematical models describing fetal heart development but they warrant revalidation and adjustment. 377 normal fetuses with healthy mothers, 98 normal fetuses with diabetic mothers, and 37 fetuses with cardiomyopathy and diabetic mothers had their cardiac structural dimensions, cardiothoracic ratio, valve flow velocities, and heart rates measured by fetal ECHO in a retrospective chart review. Cardiac features were fitted to linear functions, with respect to gestational age, femur length, head circumference, and biparietal diameter and z-scores were created to model normal fetal growth for all parameters. These z-scores were used to assess what metrics had no difference in means between the normal fetuses of both healthy and diabetic mothers but differed from those diagnosed with cardiomyopathy. It was found that functional metrics like mitral and tricuspid E wave and pulmonary velocity could be important predictors for cardiomyopathy when fitted by gestational age, femur length, head circumference, and biparietal diameter. Additionally, aortic and tricuspid annulus diameters when fitted to estimated gestational age showed potential to be predictors for fetal cardiomyopathy. While the metrics overlapped over their full range, combining them together may have the potential for predicting cardiomyopathy in utero. Future directions of this study will explore creating a classifier model that can predict cardiomyopathy using the metrics assessed in this study.

ContributorsMishra, Shambhavi (Co-author) / Numani, Asfia (Co-author) / Sweazea, Karen (Thesis director) / Plasencia, Jonathan (Committee member) / Economics Program in CLAS (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
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Description

2D fetal echocardiography (ECHO) can be used for monitoring heart development in utero. This study’s purpose is to empirically model normal fetal heart growth and function changes during development by ECHO and compare these to fetuses diagnosed with and without cardiomyopathy with diabetic mothers. There are existing mathematical models describing

2D fetal echocardiography (ECHO) can be used for monitoring heart development in utero. This study’s purpose is to empirically model normal fetal heart growth and function changes during development by ECHO and compare these to fetuses diagnosed with and without cardiomyopathy with diabetic mothers. There are existing mathematical models describing fetal heart development but they warrant revalidation and adjustment. 377 normal fetuses with healthy mothers, 98 normal fetuses with diabetic mothers, and 37 fetuses with cardiomyopathy and diabetic mothers had their cardiac structural dimensions, cardiothoracic ratio, valve flow velocities, and heart rates measured by fetal ECHO in a retrospective chart review. Cardiac features were fitted to linear functions, with respect to gestational age, femur length, head circumference, and biparietal diameter and z-scores were created to model normal fetal growth for all parameters. These z-scores were used to assess what metrics had no difference in means between the normal fetuses of both healthy and diabetic mothers, but differed from those diagnosed with cardiomyopathy. It was found that functional metrics like mitral and tricuspid E wave and pulmonary velocity could be important predictors for cardiomyopathy when fitted by gestational age, femur length, head circumference, and biparietal diameter. Additionally, aortic and tricuspid annulus diameters when fitted to estimated gestational age showed potential to be predictors for fetal cardiomyopathy. While the metrics overlapped over their full range, combining them together may have the potential for predicting cardiomyopathy in utero. Future directions of this study will explore creating a classifier model that can predict cardiomyopathy using the metrics assessed in this study.

ContributorsNumani, Asfia (Co-author) / Mishra, Shambhavi (Co-author) / Sweazea, Karen (Thesis director) / Plasencia, Jon (Committee member) / School of Mathematical and Statistical Sciences (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
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Description

The history of Arizona is filled with ambitious pioneers, courageous Natives, and loyal<br/>soldiers, but there is a seeming disconnect between those who came before us and many of those<br/>who currently inhabit this space. Many historic locations that are vital to discovering the past in<br/>Arizona are both hard to find and

The history of Arizona is filled with ambitious pioneers, courageous Natives, and loyal<br/>soldiers, but there is a seeming disconnect between those who came before us and many of those<br/>who currently inhabit this space. Many historic locations that are vital to discovering the past in<br/>Arizona are both hard to find and lacking in information pertaining to what happened there.<br/>However, despite the apparent lack of history and knowledge pertaining to these locations, they<br/>are vitally present in the public memory of the region, and we wish to shed some much-needed<br/>light on a few of these locations and the historical takeaways that can be gleaned from their<br/>study. This thesis argues the significance of three concepts: place-making, public memory, and<br/>stories. Place-making is the reinvention of history in the theater of mind which creates a<br/>plausible reality of the past through what is known in the present. Public memory is a way to<br/>explain how events in a location affect the public consciousness regarding that site and further<br/>events that stem from it. Lastly, stories about a place and event help to explain its overall impact<br/>and what can be learned from the occurrences there. Throughout this thesis we will be discussing<br/>seven sites across Arizona, the events that occurred there, and how these three aspects of study<br/>can be used to experience history in a personal way that gives us a special perspective on the<br/>land around us. The importance of personalizing history lies in finding our own identity as<br/>inhabitants of this land we call home and knowing the stories gives us greater attachment to the<br/>larger narrative of humanity as it has existed in this space.

ContributorsMartin, Trevor James (Co-author) / Martin, Austin (Co-author) / Fixico, Donald (Thesis director) / O'Donnell, Catherine (Committee member) / Dean, W.P. Carey School of Business (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
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Description

The privatization of prisons within Arizona has been a hot button issue and needs to be further analyzed to determine their benefits and expenses. To begin this investigation into the effectiveness of private prisons within Arizona and possible steps that need to be taken for its rehabilitation certain definitions and

The privatization of prisons within Arizona has been a hot button issue and needs to be further analyzed to determine their benefits and expenses. To begin this investigation into the effectiveness of private prisons within Arizona and possible steps that need to be taken for its rehabilitation certain definitions and prior research need to be understood. Following this explanation, areas such as costs analysis across different types of prisons, liability rates across differing types of prison guards, and the differing psychology of different types of prisons can be examined to gain an overall assessment of the current performance of privatized prisons within Arizona. After this in-depth analysis of the current private prison system within Arizona, the flaws of the system will become abundantly clear and the solutions that should be implemented to alleviate these problems will be discussed.

ContributorsPeters, Grayson Allen (Author) / Rigoni, Adam (Thesis director) / Schuh, Carl (Committee member) / Dean, W.P. Carey School of Business (Contributor, Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
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Description
Over the past few years, legislation legalizing sports betting in many states has been voted into effect. In the spring of 2021, Arizona legislators approved a bill that would legalize sports betting for anyone over the age of 21 in Arizona, and with Governor Doug Ducey signing the bill into

Over the past few years, legislation legalizing sports betting in many states has been voted into effect. In the spring of 2021, Arizona legislators approved a bill that would legalize sports betting for anyone over the age of 21 in Arizona, and with Governor Doug Ducey signing the bill into law, September 9, 2021, marked the first day of legal sports betting in Arizona. Due to this new major avenue of business, sports betting became a very popular topic in both the news and social media, creating a buzz for new users in Arizona. This study will focus on the sports betting industry and the major companies involved in creating sports betting infrastructure in Arizona. Data will be collected and analyzed from multiple sources including company financial statements, news articles, and independent research focusing on the financial impact of Arizona sports bettors on the sports betting industry’s market profitability. It is expected that there will be a correlation between money gambled in Arizona and a rapid increase in profitability for sports betting companies. This study will provide an overview of the economic impact that sports betting had in Arizona in 2021, and look towards the future of betting in years to come.
ContributorsMorici, Samuel (Author) / Kramer, Michael (Co-author) / Bonfiglio, Thomas (Thesis director) / Voutsas, Konstandinos (Committee member) / Barrett, The Honors College (Contributor) / Department of Finance (Contributor) / Department of Information Systems (Contributor) / Dean, W.P. Carey School of Business (Contributor)
Created2022-05
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Description
Above and Beyond ASU's Campus is a blog created for college students at Arizona State University in order to suggest opportunities to explore in Arizona out of the Tempe region. The purpose of this blog is to encourage students to go and gain life experiences that cannot be found directly

Above and Beyond ASU's Campus is a blog created for college students at Arizona State University in order to suggest opportunities to explore in Arizona out of the Tempe region. The purpose of this blog is to encourage students to go and gain life experiences that cannot be found directly on campus and allow them to make the most of their time in this desert landscape. The blog is divided into three categories consisting of outdoor activities, historical locations, and popular culture events. The blog is written from both my first-person point of view along with researched information in regard to each activity. The goal of the blog is to be relatable to the student as well as be a one-stop, easy format for finding a variety of information. The blog format was chosen in order to appeal to the target audience with its appearance, simple format, and ability to write in a relatable tone.
ContributorsDollarhide, Hannah Marie (Author) / Lynch, Jacquelyn (Thesis director) / McWilliams, Claire (Committee member) / Dean, W.P. Carey School of Business (Contributor, Contributor) / Barrett, The Honors College (Contributor)
Created2020-05
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Description
Birds maintain resting plasma glucose concentrations (pGlu) nearly twice that of comparably sized mammals. Despite this, birds do not incur much of the oxidative tissue damage that might be expected from a high pGlu. Their ability to stave off oxidative damage allows birds to serve as a negative model of

Birds maintain resting plasma glucose concentrations (pGlu) nearly twice that of comparably sized mammals. Despite this, birds do not incur much of the oxidative tissue damage that might be expected from a high pGlu. Their ability to stave off oxidative damage allows birds to serve as a negative model of hyperglycemia-related complications, making them ideal for the development of new diabetes treatments with the potential for human application. Previous studies conducted by the Sweazea Lab at Arizona State University aimed to use diet as a means to raise blood glucose in mourning doves (Zenaida macroura) in order to better understand the mechanisms they utilize to stave off oxidative damage. These protocols used dietary interventions—a 60% high fat (HF) “chow” diet, and a high carbohydrate (HC) white bread diet—but were unsuccessful in inducing pathologies. Based on this research, we hypothesized that a model of an urban diet (high in fat, refined carbohydrates, and sodium) might impair vasodilation, as the effect of this diet on birds is currently unknown. We found that tibial vasodilation was significantly impaired in birds fed an urban diet compared to those fed a seed diet. Unexpectedly, vasodilation in the urban diet group was comparable to data of wild-caught birds from previous research, possibly indicating that the birds had already been eating a diet similar to this study’s urban diet before they were caught. This may constitute evidence that the seed diet improved vasodilation while the urban diet more closely mimicked the diet of the birds before the trial, suggesting that the model of the urban diet acted as the control diet in this context. This study is the first step in elucidating avian mechanisms for dealing with diabetogenic diets and has potential to aid in the development of treatments for humans with metabolic syndrome.
ContributorsRenner, Michael William (Author) / Sweazea, Karen (Thesis director) / Johnston, Carol (Committee member) / Basile, Anthony (Committee member) / Dean, W.P. Carey School of Business (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05
Description
Patients with type 2 diabetes mellitus experience a slower healing process and poor osteointegration, making it difficult for them to heal properly after a bone fracture. This study aims to compare the proliferation and differentiation of human mesenchymal stromal cells at different glucose concentrations, as well as with an advanced

Patients with type 2 diabetes mellitus experience a slower healing process and poor osteointegration, making it difficult for them to heal properly after a bone fracture. This study aims to compare the proliferation and differentiation of human mesenchymal stromal cells at different glucose concentrations, as well as with an advanced glycated end-product (AGE) concentration, to mimic a healthy, prediabetic, and diabetic environment in an in vitro model over several experiments. Each experiment was composed of treatment groups in either growth or osteogenic media, with varying levels of glucose concentration or an advanced glycated end-product concentration. The treatment groups were cultured in 24 well plates over 28 days with staining of FITC-maleimide, DAPI, or alkaline phosphatase conducted at varying time points. The plates were imaged, then analyzed in ImageJ and GraphPad Prism. The study supports that at 28 days in culture, the more glucose added to osteogenic media treatment groups, the lower the nuclear count. At 14 days the same is true of growth media treatment groups, though the trend does not persist until 28 days. It does not seem that cell surface area of osteogenic groups, and growth media treatment groups was affected by glucose level. At 14 days, the alkaline phosphatase expression was unaffected by glucose level. However, at the 28 day time point the higher the glucose level of osteogenic treatment groups, the less expression of alkaline phosphatase. The effect of the added AGE concentration on hMSC osteogenesis was inconclusive. Overall, this study enhanced understanding of the role that glucose and AGEs play in the bone healing process for diabetic patients, allowing for future improvements of biomaterials and engineered tissue.
ContributorsMoya, Adriana Allyssa (Author) / Holloway, Julianne (Thesis director) / Fumasi, Fallon (Committee member) / Dean, W.P. Carey School of Business (Contributor) / Chemical Engineering Program (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
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DescriptionThis paper takes a comprehensive approach to evaluate Arizona’s conformity process after the passage of TCJA in December 2017.
ContributorsBowman, Anika (Author) / Frost, Donald (Thesis director) / Dallmus, John (Committee member) / School of Accountancy (Contributor) / Dean, W.P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05