Matching Items (478)

Filtering by

Clear all filters

131389-Thumbnail Image.png

Managerial Factors in Effective Workplace Communication: Analyzing the effects of Tailoring Communication Styles and Verbalizing Expectations in the Workplace

Description

This project analyzes the large array of managerial leadership research in congruence with the wide field of workplace communication to attempt to determine the importance of refining communication channels between managers and employees as well as articulate the core competencies

This project analyzes the large array of managerial leadership research in congruence with the wide field of workplace communication to attempt to determine the importance of refining communication channels between managers and employees as well as articulate the core competencies a manager should exhibit when practicing exemplary communication in their respective work environment. The preliminary sections of this thesis will establish the currently existing foundations utilized and narrow the wide range of research available to applicable information regarding positive workplace communication, influencing factors in a feedback loop from the employee’s perspective, as well as leadership aspects and actions a manager can alter or initiate to improve their workplace’s environment through communicational refinement. This research is supplemented with a survey that was administered to Arizona State University student leaders who were involved in coordinating the Regional Business Conference on the Polytechnic campus. The survey data is designed to either confirm or contradict the findings of the literature. The objective of this project is to synthesize an overview of a manager’s responsibilities and recommend actions to tailor and improve workplace communication

Contributors

Created

Date Created
2020-05

133894-Thumbnail Image.png

Pandora: A Play by Abbey Toye

Description

Pandora is a play exploring our relationship with gendered technology through the lens of artificial intelligence. Can women be subjective under patriarchy? Do robots who look like women have subjectivity? Hoping to create a better version of ourselves, The Engineer

Pandora is a play exploring our relationship with gendered technology through the lens of artificial intelligence. Can women be subjective under patriarchy? Do robots who look like women have subjectivity? Hoping to create a better version of ourselves, The Engineer must navigate the loss of her creation, and Pandora must navigate their new world. The original premiere run was March 27-28, 2018, original cast: Caitlin Andelora, Rikki Tremblay, and Michael Tristano Jr.

Contributors

Agent

Created

Date Created
2018-05

133727-Thumbnail Image.png

Tongues Becoming a Virtuous Woman: A Philosophical and Communicative Approach to Young Women's Speech

Description

Four hundred years after the word "virtuous," came to be associated with a woman's sexuality, today's female adolescent seemingly has everything. Yet, there is a psychological civil war raging in the psyche of the 21st century young American female because

Four hundred years after the word "virtuous," came to be associated with a woman's sexuality, today's female adolescent seemingly has everything. Yet, there is a psychological civil war raging in the psyche of the 21st century young American female because her mind is divided against itself due to the conflicting instructions of who and what she should be. She has so many choices; it is easy to become overwhelmed by them. Today's female youth is threatened. She communicates more and more, but her ability to express herself is inhibited because she is unsure of how to develop an authentic sense of self. It is a hermeneutic understanding of communication and what it means to be "virtuous" that can free young women to cultivate authentic self and continue to make decisions that support such a lifestyle. It is the aim of this thesis to reclaim the word "virtuous" for the benefit of today's young women. Deeper understanding of hermeneutics and communication allow us to view this word in a different light and read the entirety of Proverbs 31 as feminists. Young women have always faced challenges in adolescence, but a return to classical wisdom and philosophy will equip them to further advance themselves and their communities, rather than forcing them into a life of speaking tongue twisters. The virtuous young woman does not know what the future holds, but armed with the lessons of tradition and the fire of hope, she may speak a virtuous magic over the world with a tongue fit for the challenge.

Contributors

Agent

Created

Date Created
2018-05

133850-Thumbnail Image.png

Communication Strategies for Effective Social Media Use in Local Governments

Description

An information influx and numerous modes of content delivery has resulted in local governments competing for the public's attention. A recent poll from the Public Technology Institute discovered that although 85% of Local Governments use social media to disseminate information

An information influx and numerous modes of content delivery has resulted in local governments competing for the public's attention. A recent poll from the Public Technology Institute discovered that although 85% of Local Governments use social media to disseminate information to their constituents, only 37% have an enterprise-wide social media strategy (PTI, 2017). Without a clear approach towards social media, Local Governments are failing to maximize their voices and often ineffective when reaching out to their constituents. Research has suggested, charisma is a successful tool for capturing an audience's attention and conveying a memorable message. Charisma can also be taught and executed not only through spoken rhetoric but in online social media platforms. Within this study, 18 local government employees participated in an educational workshop on the use of nine non-verbal "Charismatic Leadership Tactics". Participants completed a pre-workshop assignment which was later compared to a post-workshop assignment. Results showed, participants on average, increased their use of Charismatic Leadership Tactics by a mean of 61%. Researchers collected social media analytics one month prior and one month following the workshop from the City's social media accounts in which participants managed. Collectively, of the thirteen social media accounts, the overall total engagement was greater the month after the educational workshop compared to the month before the workshop. These results suggest charisma can be taught, charisma can be conveyed through micro-blogosphere platforms such as Twitter, and the use of Charismatic Leadership Tactics could be responsible for increasing follower engagement with social media content.

Contributors

Agent

Created

Date Created
2018-05

132463-Thumbnail Image.png

S. Cerevisiae Sul1 and Sul2 Sulfate Transporters in Varying Sulfate Concentrations

Description

The primary objective of this project is to further the knowledge about SCL26 family of anion transporters. The goals of the experiment were to find the lowest sulfate concentration where the yeast without Sulp1 and Sulp2 is able to grow,

The primary objective of this project is to further the knowledge about SCL26 family of anion transporters. The goals of the experiment were to find the lowest sulfate concentration where the yeast without Sulp1 and Sulp2 is able to grow, but it grows very slowly, and to find a higher sulfate concentration where the yeast grows quickly, with or without the sulfate transporters. The lowest sulfate concentration where the yeast without the sulfate transporters is able to grow was determined to be 2-4 mM, however, this range can likely be refined by more quantitative analytical methods. At a sulfate concentration of 20 mM sulfate or higher, the yeast is able to grow quickly without high-affinity sulfate transporters. The next step in the project is to re-introduce the Sulp1 and Sulp2 genes into the yeast, so that growth in low and high sulfate conditions can be compared with and without the Sulp1 and Sulp2 proteins. The long-term goals of the project are to bring experience with yeast to Dr. Nannenga’s structural discovery lab, to determine if yeast sulfate transporters respond in the same way to drug candidates as human sulfate transporters, and to determine the structure of the proteins using cryo-electron microscopy.

Contributors

Agent

Created

Date Created
2019-05

133245-Thumbnail Image.png

Measuring the Activity of Φ29 DNA Polymerase

Description

The major goal of this large project is to develop a Recognition Tunneling Nanopore (RTP) device that will be used for determining the structure of glycosaminoglycans (GAGs). The RTP device is composed of a recognition tunneling junction that is embedded

The major goal of this large project is to develop a Recognition Tunneling Nanopore (RTP) device that will be used for determining the structure of glycosaminoglycans (GAGs). The RTP device is composed of a recognition tunneling junction that is embedded in a nanopore. In order to translocate the GAG molecule through the nanopore, researchers have designed a scheme in which the GAG molecule of interest will be attached to the 5’ end of a DNA primer (figure 1) and the DNA primer will be extended by a biotinylated Φ29 DNA polymerase that is anchored in the nanoslit using streptavidin. This research project specifically is part of a larger project with the main goal of comparing the activity of the wild-type Φ29 DNA polymerase which I have expressed and purified with the mutated Φ29 DNA polymerase devoid of 3’ - 5’ exonuclease activity which was made by Dr. Deng.

Contributors

Agent

Created

Date Created
2018-05

133038-Thumbnail Image.png

Analysis of the Cellular Localization of PANK2 Mutations Using a Yeast Model

Description

Pantothenate kinase-associated neurodegeneration, PKAN, is a neurological disease that is caused by biallelic mutations in the PANK2 gene, which codes for a pantothenate kinase. Some PANK2 mutations that cause PKAN retain enzymatic activity. A possible explanation for the mutations that

Pantothenate kinase-associated neurodegeneration, PKAN, is a neurological disease that is caused by biallelic mutations in the PANK2 gene, which codes for a pantothenate kinase. Some PANK2 mutations that cause PKAN retain enzymatic activity. A possible explanation for the mutations that have residual activity but still cause the disease is that they do not have the correct cellular localization. The localization of PANK2 was studied through cellular fractionation. We found the precursor form of PANK2, pPANK2, appears to be anchored to the inner membrane of the mitochondria, and the mature form, mPANK2, is located in the inter-membrane space, IMS. However, the IMS of the PKAN causing mutants is completely devoid of mPANK2 which suggests some disease-causing mutations may be mislocalized. In addition, PANK2 catalyzes the first and rate limiting step in Coenzyme A biosynthesis, and in other studies, it has been shown that the CoA biosynthesis enzymes form a complex in yeast. Therefore, we also considered the possibility that PKAN-causing mutations that retain activity have altered interactions with the other CoA biosynthesis enzymes. Coimmunoprecipitation of the proteins in the pathway was done to determine if there were any interactions with PANK2. The results indicate that PANK2 does not directly interact with either PPCS or CoASY, the second and final enzymatic activities in the CoA biosynthesis pathway.

Contributors

Agent

Created

Date Created
2019-05

133471-Thumbnail Image.png

Structural Analysis of the Spinach Rubisco Activase AAA+ Domain by Negative Stain Electron Microscopy

Description

Higher plant Rubisco activase (Rca) is a stromal ATPase responsible for reactivating Rubisco. It is a member of the AAA+ protein superfamily and is thought to assemble into closed-ring hexamers like other AAA+ proteins belonging to the classic clade. Progress

Higher plant Rubisco activase (Rca) is a stromal ATPase responsible for reactivating Rubisco. It is a member of the AAA+ protein superfamily and is thought to assemble into closed-ring hexamers like other AAA+ proteins belonging to the classic clade. Progress towards modeling the interaction between Rca and Rubisco has been slow due to limited structural information on Rca. Previous efforts in the lab were directed towards solving the structure of spinach short-form Rca using X-ray crystallography, given that it had notably high thermostability in the presence of ATP-γS, an ATP analog. However, due to disorder within the crystal lattice, an atomic resolution structure could not be obtained, prompting us to move to negative stain electron microscopy (EM), with our long-term goal being the use of cryo-electron microscopy (cryo-EM) for atomic resolution structure determination. Thus far, we have screened different Rca constructs in the presence of ATP-γS, both the full-length β-isoform and truncations containing only the AAA+ domain. Images collected on preparations of the full-length protein were amorphous, whereas images of the AAA+ domain showed well-defined ring-like assemblies under some conditions. Procedural adjustments, such as the use of previously frozen protein samples, rapid dilution, and minimizing thawing time were shown to improve complex assembly. The presence of Mn2+ was also found to improve hexamer formation over Mg2+. Calculated class averages of the AAA+ Rca construct in the presence of ATP-γS indicated a lack of homogeneity in the assemblies, showing both symmetric and asymmetric hexameric rings. To improve structural homogeneity, we tested buffer conditions containing either ADP alone or different ratios of ATP-γS to ADP, though results did not show a significant improvement in homogeneity. Multiple AAA+ domain preparations were evaluated. Because uniform protein assembly is a major requirement for structure solution by cryo-EM, more work needs to be done on screening biochemical conditions to optimize homogeneity.

Contributors

Agent

Created

Date Created
2018-05

133531-Thumbnail Image.png

Algorithmic Prediction of Binding Sites of TNFα/TNFR2 and PD-1/PD-L1

Description

Predicting the binding sites of proteins has historically relied on the determination of protein structural data. However, the ability to utilize binding data obtained from a simple assay and computationally make the same predictions using only sequence information would be

Predicting the binding sites of proteins has historically relied on the determination of protein structural data. However, the ability to utilize binding data obtained from a simple assay and computationally make the same predictions using only sequence information would be more efficient, both in time and resources. The purpose of this study was to evaluate the effectiveness of an algorithm developed to predict regions of high-binding on proteins as it applies to determining the regions of interaction between binding partners. This approach was applied to tumor necrosis factor alpha (TNFα), its receptor TNFR2, programmed cell death protein-1 (PD-1), and one of its ligand PD-L1. The algorithms applied accurately predicted the binding region between TNFα and TNFR2 in which the interacting residues are sequential on TNFα, however failed to predict discontinuous regions of binding as accurately. The interface of PD-1 and PD-L1 contained continuous residues interacting with each other, however this region was predicted to bind weaker than the regions on the external portions of the molecules. Limitations of this approach include use of a linear search window (resulting in inability to predict discontinuous binding residues), and the use of proteins with unnaturally exposed regions, in the case of PD-1 and PD-L1 (resulting in observed interactions which would not occur normally). However, this method was overall very effective in utilizing the available information to make accurate predictions. The use of the microarray to obtain binding information and a computer algorithm to analyze is a versatile tool capable of being adapted to refine accuracy.

Contributors

Agent

Created

Date Created
2018-05

133424-Thumbnail Image.png

Educating Future Engineers in the Art of Communication through Presenting

Description

Effective communication and engineering are not a natural pairing. The incongruence is because engineering students are focused on making, designing and analyzing. Since these are the core functions of the field there is not a direct focus on developing communication

Effective communication and engineering are not a natural pairing. The incongruence is because engineering students are focused on making, designing and analyzing. Since these are the core functions of the field there is not a direct focus on developing communication skills. This honors thesis explores the role and expectations for student engineers within the undergraduate engineering education experience to present and communicate ideas. The researchers interviewed faculty about their perspective on students' abilities with respect to their presentation skills to inform the design of a workshop series of interventions intended to make engineering students better communicators.

Contributors

Agent

Created

Date Created
2018-05