Human Immunodeficiency Virus (HIV) remains a persistent problem around the world, even though antiretroviral therapy has shown to be effective in reducing viral load and limiting transmission of the virus. Due to HIV’s infectious nature, visibility, the populations at risk, and its connections to race, class, and sexuality, it is more stigmatized than any other illness. HIV stigma has been associated with increased depression, social isolation, and poor psychological adjustment. HIV stigma can influence disclosure and care-seeking behavior. Internet-based interventions have shown to be effective in increasing knowledge on STIs and HIV, however, researchers have tested strategies that include educating participants on HIV to reduce stigma and have found that informational approaches alone are not effective. There is evidence that emotional intelligence and empathy are associated with prosocial behavior and influence attitudes towards stigmatized groups. Thus, this thesis aims to test an online intervention using an informational video from the Center for Disease Control and Prevention (CDC) in combination with an empathy-generating component to reduce stigma. It was hypothesized that the online intervention would increase HIV knowledge scores (H1), but stigma will only be reduced in the group introduced to the empathy-inducing component (H2) and those with high emotional intelligence would show the greatest reduction in stigmatizing attitudes (H3). Results did not support these hypotheses, suggesting that the CDC’s video does not significantly increase HIV knowledge in the general public. Further, the video intended to generate empathy and reduce stigma was also ineffective. These findings stress the need for further research and questions the effectiveness of empathy-generating interventions (e.g., FACES OF HIV, HIV Justice Network) to increase knowledge and reduce stigma. Future researchers should test the effectiveness of personalized interventions to reduce HIV-related stigma.