Matching Items (9)
Description
Biofuel from microbial biomass is a viable alternative to current energy production practices that could mitigate greenhouse gas levels and reduce dependency on fossil fuels. Sustainable production of microbial biomass requires efficient utilization of nutrients like phosphorus (P). P is a limited resource which is vital for global food security. This paper seeks to understand the fate of P through biofuel production and proposes a proof-of-concept process to recover P from microbial biomass. The photosynthetic cyanobacterium Synechocystis sp. PCC 6803 is found to contain 1.4% P by dry weight. After the crude lipids are extracted for biofuel processing, 92% of the intercellular P is found within the residual biomass. Most intercellular P is associated with nucleic acids which remain within the cell after lipids are extracted. Phospholipids comprise a small percentage of cellular P. A wet chemical advanced oxidation process of adding 30% hydrogen peroxide followed by 10 min of microwave heating converts 92% of the total cellular P from organic-P and polyphosphate into orthophosphate. P was then isolated and concentrated from the complex digested matrix by use of resins. An anion exchange resin impregnated with iron nanoparticles demonstrates high affinity for P by sorbing 98% of the influent P through 20 bed volumes, but only was able to release 23% of it when regenerated. A strong base anion exchange resin sorbed 87% of the influent P through 20 bed volumes then released 50% of it upon regeneration. The overall P recovery process was able to recover 48% of the starting intercellular P into a pure and concentrated nutrient solution available for reuse. Further optimization of elution could improve P recovery, but this provides a proof-of-concept for converting residual biomass after lipid extraction to a beneficial P source.
ContributorsGifford, James McKay (Author) / Westerhoff, Paul (Thesis advisor) / Rittmann, Bruce (Committee member) / Vannela, Ravindhar (Committee member) / Arizona State University (Publisher)
Created2012
Description
Overexpression of AVP1 (Arabidopsis vacuolar pyrophosphatase), a type I H+ pyrophosphatase, results in greater biomass, possibly due to a function in sucrose transport within the phloem. Overexpression of the phloem lipid-associated family protein (PLAFP) was shown to increase the number of vascular bundles in Arabidopsis. Could these two phenotypes complement one another additively? In this work, double mutants overexpressing both AVP1 and PLAFP were characterized. These double mutants have enhanced biomass, greater leaf area, and a larger number of vascular bundles than the single mutant lines. Overexpression of PLAFP does not result in any increase in rhizosphere acidification capacity.
ContributorsWilson, Sean (Co-author) / Furstenau, Tara (Co-author) / Gaxiola, Roberto (Thesis director) / Mason, Hugh (Committee member) / Wojciechowski, Martin (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor) / School of Life Sciences (Contributor)
Created2014-05
Description
The inability of a single strain of bacteria to simultaneously and completely consume multiple sugars, such as glucose and xylose, hinder industrial microbial processes for ethanol and lactate production. To overcome this limitation, I am engineering an E. coli co-culture system consisting of two ‘specialists'. One has the ability to only consume xylose and the other only glucose. This allows for co-utilization of lignocellulose-derived sugars so both sugars are completely consumed, residence time is reduced and lactate and ethanol titers are maximized.
ContributorsAyla, Zeynep Ece (Author) / Nielsen, David (Thesis director) / Flores, Andrew (Committee member) / Chemical Engineering Program (Contributor) / School of Sustainability (Contributor) / Barrett, The Honors College (Contributor)
Created2017-05
Description
Unmanned aerial vehicles have received increased attention in the last decade due to their versatility, as well as the availability of inexpensive sensors (e.g. GPS, IMU) for their navigation and control. Multirotor vehicles, specifically quadrotors, have formed a fast growing field in robotics, with the range of applications spanning from surveil- lance and reconnaissance to agriculture and large area mapping. Although in most applications single quadrotors are used, there is an increasing interest in architectures controlling multiple quadrotors executing a collaborative task. This thesis introduces a new concept of control involving more than one quadrotors, according to which two quadrotors can be physically coupled in mid-flight. This concept equips the quadro- tors with new capabilities, e.g. increased payload or pursuit and capturing of other quadrotors. A comprehensive simulation of the approach is built to simulate coupled quadrotors. The dynamics and modeling of the coupled system is presented together with a discussion regarding the coupling mechanism, impact modeling and additional considerations that have been investigated. Simulation results are presented for cases of static coupling as well as enemy quadrotor pursuit and capture, together with an analysis of control methodology and gain tuning. Practical implementations are introduced as results show the feasibility of this design.
ContributorsLarsson, Daniel (Author) / Artemiadis, Panagiotis (Thesis advisor) / Marvi, Hamidreza (Committee member) / Berman, Spring (Committee member) / Arizona State University (Publisher)
Created2016
Description
There has been a vast increase in applications of Unmanned Aerial Vehicles (UAVs) in civilian domains. To operate in the civilian airspace, a UAV must be able to sense and avoid both static and moving obstacles for flight safety. While indoor and low-altitude environments are mainly occupied by static obstacles, risks in space of higher altitude primarily come from moving obstacles such as other aircraft or flying vehicles in the airspace. Therefore, the ability to avoid moving obstacles becomes a necessity
for Unmanned Aerial Vehicles.
Towards enabling a UAV to autonomously sense and avoid moving obstacles, this thesis makes the following contributions. Initially, an image-based reactive motion planner is developed for a quadrotor to avoid a fast approaching obstacle. Furthermore, A Dubin’s curve based geometry method is developed as a global path planner for a fixed-wing UAV to avoid collisions with aircraft. The image-based method is unable to produce an optimal path and the geometry method uses a simplified UAV model. To compensate
these two disadvantages, a series of algorithms built upon the Closed-Loop Rapid Exploratory Random Tree are developed as global path planners to generate collision avoidance paths in real time. The algorithms are validated in Software-In-the-Loop (SITL) and Hardware-In-the-Loop (HIL) simulations using a fixed-wing UAV model and in real flight experiments using quadrotors. It is observed that the algorithm enables a UAV to avoid moving obstacles approaching to it with different directions and speeds.
for Unmanned Aerial Vehicles.
Towards enabling a UAV to autonomously sense and avoid moving obstacles, this thesis makes the following contributions. Initially, an image-based reactive motion planner is developed for a quadrotor to avoid a fast approaching obstacle. Furthermore, A Dubin’s curve based geometry method is developed as a global path planner for a fixed-wing UAV to avoid collisions with aircraft. The image-based method is unable to produce an optimal path and the geometry method uses a simplified UAV model. To compensate
these two disadvantages, a series of algorithms built upon the Closed-Loop Rapid Exploratory Random Tree are developed as global path planners to generate collision avoidance paths in real time. The algorithms are validated in Software-In-the-Loop (SITL) and Hardware-In-the-Loop (HIL) simulations using a fixed-wing UAV model and in real flight experiments using quadrotors. It is observed that the algorithm enables a UAV to avoid moving obstacles approaching to it with different directions and speeds.
ContributorsLin, Yucong (Author) / Saripalli, Srikanth (Thesis advisor) / Scowen, Paul (Committee member) / Fainekos, Georgios (Committee member) / Thangavelautham, Jekanthan (Committee member) / Youngbull, Cody (Committee member) / Arizona State University (Publisher)
Created2015
Description
Biomass synthesis is a competing factor in biological systems geared towards generation of commodity and specialty chemicals, ultimately limiting maximum titer and yield; in this thesis, a widely generalizable, modular approach focused on decoupling biomass synthesis from the production of the phenylalanine in a genetically modified strain of E. coli BW25113 was explored with the use of synthetic trans-encoded small RNA (sRNA) to achieve greater efficiency. The naturally occurring sRNA MicC was used as a scaffold, and combined on a plasmid with a promoter for anhydrous tetracycline (aTc) and a T1/TE terminator. The coding sequence corresponding to the target binding site for fourteen potentially growth-essential gene targets as well as non-essential lacZ was placed in the seed region of the of the sRNA scaffold and transformed into BW25113, effectively generating a unique strain for each gene target. The BW25113 strain corresponding to each gene target was screened in M9 minimal media; decreased optical density and elongated cell morphology changes were observed and quantified in all induced sRNA cases where growth-essential genes were targeted. Six of the strains targeting different aspects of cell division that effectively suppressed growth and resulted in increased cell size were then screened for viability and metabolic activity in a scaled-up shaker flask experiment; all six strains were shown to be viable during stationary phase, and a metabolite analysis showed increased specific glucose consumption rates in induced strains, with unaffected specific glucose consumption rates in uninduced strains. The growth suppression, morphology and metabolic activity of the induced strains in BW25113 was compared to the bacteriostatic additives chloramphenicol, tetracycline, and streptomycin. At this same scale, the sRNA plasmid targeting the gene murA was transformed into BW25113 pINT-GA, a phenylalanine overproducer with the feedback resistant genes aroG and pheA overexpressed. Two induction times were explored during exponential phase, and while the optimal induction time was found to increase titer and yield amongst the BW25113 pINT-GA murA sRNA variant, overall this did not have as great a titer or yield as the BW25113 pINT-GA strain without the sRNA plasmid; this may be a result of the cell filamentation.
ContributorsHerschel, Daniel Jordan (Author) / Nielsen, David R (Thesis advisor) / Torres, César I (Committee member) / Wang, Xuan (Committee member) / Arizona State University (Publisher)
Created2016
Description
Climate change has the potential to reduce the amount of land that is suitable for crop growth. Such changes may cause food shortages, which would most likely disproportionately affect the poorest regions of the world. While GMO crops showed potential to increase crop yield and agricultural efficiency, significant public pushback has led to a search for alternative methods to generate similar results. Compounds produced by bacteria, such as 2,3-butanediol, offer a potential way to change the phenotypes of plants without the deliberate genomic changes involved in the development of GMOs which are often the subject of great controversy. These compounds influence how plants grow and function. Through precise application, the compounds could be used to improve crop yield and stress tolerance. While these effects are not completely understood, they may be due to changes in transcription and translation of certain proteins, the microbiome surrounding the plants and its interactions with the compounds, or other unknown factors. The compound 2,3-butanediol appears to increase biomass, lead to larger root systems and more root hairs, and increase germination rates in a variety of plants. All these traits are favorable for producing higher yields and enduring stress conditions. The phenotypes induced by this compound are similar to plants engineered to over express a type I proton pyrophosphatase. Plants treated with 2,3-butanediol offer a potential option to achieve the benefits of GMO crops without the attached social stigma.
ContributorsOlson, Erik Jon (Co-author) / Olson, Erik (Co-author) / Gaxiola, Roberto (Thesis director) / Mason, Hugh (Committee member) / Riley, James (Committee member) / School of Life Sciences (Contributor) / Economics Program in CLAS (Contributor) / Barrett, The Honors College (Contributor)
Created2019-12
Description
In northern Arizona, the removal of woody biomass from forested land has garnered a high level of interest as threats of catastrophic wildfires have increased in recent years. Although there has been a great deal of vocal support for forest restoration, efforts on the ground are often stalled by complex federal contracting systems, a weak logging and sawmill industry, low-quality timber, and inabilities to guarantee long-term biomass supplies to processers. These barriers are exceedingly apparent in in the Flagstaff area, where the vast majority of forested land falls under the jurisdiction of the federal government and little infrastructure exists for wood product industries. In order to address these obstacles, forest stakeholders in Coconino County are actively searching for enterprises to utilize material that urgently needs to be removed from the surrounding forests. This project aimed to assist stakeholders in this endeavor by identifying and researching a number of practical and innovative woody biomass utilization enterprises that are suited to the existing regional infrastructure. While there are a variety of ways to process biomass, this project focuses on the following four end products because of their ability to use residual materials from harvest and sawmill operations, their low-tech nature, and the end product’s proximity to potential markets: biochar, compost, wood-plastic composites, and mushroom cultivation. Each of these products, and the processes used to create them, were analyzed and evaluated using a sustainable enterprise framework, and the final results were summarized in a portfolio for stakeholders in the region to review. Although this project offered just a glimpse of what is possible, the ultimate aim was to foster collaborative conversations regarding how forest restoration residues can be used in sustainable and innovative ways.
ContributorsPaulus, Caitlin (Contributor)
Created2019-05-15
Description
Domestic energy is an important component of our day to day lives and is something we cannot live without. Imagine how life would be without a means to cook our food, to warm our house, life would be unbearable. As we enjoy these comforts rarely do we stop to think what the opportunity cost is. For those using renewable sources, it is not a big issue, but for those who rely on wood fuel, they have to strike a delicate balance between need for fuel and the need to conserve the greatest support systems of their livelihoods, the forests. The main source of energy for households in many developing countries is biomass, mainly from forests and woodlands. The continued use of firewood and charcoal fuel puts a strain on forests, resulting in adverse effects on the environment such as prolonged droughts, loss of biodiversity, dwindling water resources, changing weather patterns among other sustainability challenges. An alternative to firewood to charcoal lies in biochar briquettes. This paper discusses the role of biochar briquettes in mitigating climate change and serves as a step by step guide on how biochar briquettes may be produced.
ContributorsNganga, Patrick M. (Author)
Created2018