Matching Items (5)
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- All Subjects: Antibodies
- All Subjects: Empathy
- Creators: W.P. Carey School of Business
Description
This project aims to address the current protocol regarding the diagnosis and treatment of traumatic brain injury (TBI) in medical industries around the world. Although there are various methods used to qualitatively determine if TBI has occurred to a patient, this study attempts to aid in the creation of a system for quantitative measurement of TBI and its relative magnitude. Through a method of artificial evolution/selection called phage display, an antibody that binds highly specifically to a post-TBI upregulated brain chondroitin sulfate proteoglycan called neurocan has been identified. As TG1 Escheria Coli bacteria were infected with KM13 helper phage and M13 filamentous phage in conjunction, monovalent display of antibody fragments (ScFv) was performed. The ScFv bind directly to the neurocan and from screening, phage that produced ScFv's with higher affinity and specificity to neurocan were separated and purified. Future research aims to improve the ScFv characteristics through increased screening toward neurocan. The identification of a highly specific antibody could lead to improved targeting of neurocan post-TBI in-vivo, aiding researchers in quantitatively defining TBI by visualizing its magnitude.
ContributorsSeelig, Timothy Scott (Author) / Stabenfeldt, Sarah (Thesis director) / Ankeny, Casey (Committee member) / Barrett, The Honors College (Contributor) / Harrington Bioengineering Program (Contributor)
Created2015-05
Description
There are many anecdotal stories of dogs rescuing their owners from dangerous situations, but this rescue behavior has yet to be shown in an experimental setting. Studies have shown that dogs behave differently towards crying humans, but do not seek help for their owners when they are in distress. This study sought to determine if a dog could recognize when its owner was in distress and would attempt to rescue the owner. The experiment consisted of three conditions: a distress condition to determine how dogs respond to an owner calling for help, a reading condition to control for proximity-seeking and sound, and a food control to use as a basis for motivation and door-opening ability of the dog. Sixty dogs were tested in all three conditions in a pseudo-random order so that an equal number of dogs completed the conditions in each order. 38% of the dogs opened the apparatus for any condition, while 32% opened for the food and distress conditions and 27% opened for the reading condition, which shows that rescue in general is unlikely. There was no significant difference in the proportion of dogs who opened the apparatus for each condition, indicating that dogs are no more likely to rescue their distressed owners than they are to open the apparatus for other conditions and may not be able to sense that the owner is in distress. The similarities in the success rates also show that the owner can be just as motivating for a dog as food. Overall, the low success rates suggest that dogs are not generally likely to rescue a person who is trapped, even when they are calling for help.
ContributorsPatterson, Jordan Elizabeth (Author) / Wynne, Clive (Thesis director) / McBeath, Michael (Committee member) / W.P. Carey School of Business (Contributor) / Mechanical and Aerospace Engineering Program (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
A chimeric, humanized monoclonal antibody that recognizes a highly conserved fusion loop found on flaviviruses was constructed with a geminiviral replicon and transiently expressed in Nicotiana benthamiana plants through Agrobacterium tumefaciens infiltration. Characterization and expression studies were then conducted to confirm correct assembly of the antibody. Once the antibody was purified, an ELISA was conducted to validate that the antibody was able to bind to the flavivirus fusion loop.
ContributorsPardhe, Mary (Author) / Mason, Hugh (Thesis director) / Chen, Qiang (Committee member) / Mor, Tsafrir (Committee member) / School of Life Sciences (Contributor) / Department of Information Systems (Contributor) / W.P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
This project is meant to measure and assess empathy through the Empathy Assessment Index (EAI) and Social Empathy Index (SEI) instruments. Researchers believe that empathy is an involuntary but dynamic aspect of people's affective and cognitive responses to emotional stimuli. This project used the EAI and SEI instruments to see whether a course taught at Arizona State University \u2014 PAF 300 \u2014increased empathy and its seven components within students. The results suggest that different modular interventions were effective in increasing four of the seven empathic components \u2014 affective response, perspective-taking, contextual understanding of systemic barriers, and macro self-other awareness/ perspective-taking \u2014 but that it was detrimental to two components, self-other awareness and affective mentalizing. Future studies are necessary to understand how aspects of a course curriculum can target and increase the seven components in individuals, as well as how these components relate to one another within the greater concept of empathy. Still, this research is important in the greater scheme of empathy as it seeks to understand and expand individuals' empathic levels in an increasingly bleak and desolate political climate.
ContributorsPirkl, Audrie Madison (Author) / Johnston, Erik W., 1977- (Thesis director) / Minrichs, Margaret (Committee member) / W.P. Carey School of Business (Contributor) / School of Public Affairs (Contributor) / Department of Management and Entrepreneurship (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
Monoclonal antibody therapy focuses on engineering immune cells to target specific peptide sequences indicative of disease. An impediment in the continued advancement of this market is the lack of an efficient, inexpensive means of characterization that can be broadly applied to any antibody while still providing high-density data. Many characterization methods address an antibody's affinity for its cognate sequence but overlook other important aspects of binding behavior such as off-target binding interactions. The purpose of this study is to demonstrate how the binding intensity between an antibody and a library of random-sequence peptides, otherwise known as an immunosignature, can be evaluated to determine antibody specificity and polyreactivity. A total of 24 commercially available monoclonal antibodies were assayed on 125K and 330K peptide microarrays and analyzed using a motif clustering program to predict candidate epitopes within each antigen sequence. The results support the further development of immunosignaturing as an antibody characterization tool that is relevant to both therapeutic and non-therapeutic antibodies.
ContributorsDai, Jennifer T. (Author) / Stafford, Phillip (Thesis director) / Diehnelt, Chris (Committee member) / School of Life Sciences (Contributor) / W.P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05