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- All Subjects: Sustainability
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Description
My project is an examination of the process ASU Tempe campus took to institute an organics collection program. Working from a sustainability science perspective I demonstrate the structural and logistical barriers faced during program creation and expansion. My examination lead to the creation of a manual designed as a tool for other organizations in which I document ASU's process and provide information on key steps and procedures necessary to implement a unique organics collection program.
ContributorsSchumacher, Katherine Marie (Author) / Schoon, Michael (Thesis director) / Brundiers, Katja (Committee member) / Levine, Alana (Committee member) / Barrett, The Honors College (Contributor) / T. Denny Sanford School of Social and Family Dynamics (Contributor) / School of Sustainability (Contributor)
Created2013-05
Description
Dengue virus infects millions of people every year. Yet there is still no vaccine available to prevent it. Here we use a neutralizing epitope determinant on the dengue envelope (E) protein as an immunogen to be vectored by a measles virus (MV) vaccine. However the domain III (DIII) of the dengue 2 E protein is too small to be immunogenic by itself. In order for it to be displayed on a larger particle, it was inserted into the amino terminus of small hepatitis B surface antigen (HBsAg, S) coding sequence. To generate the recombinant MV vector and verify the efficiency of this concept, a reverse genetics system was used where the MV vectors express one or two additional transcription units to direct the assembly of hybrid HBsAg particles. Two types of recombinant measles virus were produced: pB(+)MVvac2(DIII-S,S)P and pB(+)MVvac2(DIII-S)N. Virus recovered from pB(+)MVvac2(DIII-S,S)P was viable. An ELISA assay was performed to demonstrate the expression and secretion of HBsAg. Supernatant from MVvac2(DIII-S,S)P infected cells confirmed that hybrid HBsAg-domain III particles with a density similar to traditional HBsAg particles were released. Characteristics of the subviral particle have been analyzed for the successful incorporation of domain III. The replication fitness of the recombinant MV was evaluated using multi-step growth kinetics and showed reduced replication fitness when compared to the parental strain MVvac2. This demonstrates that viral replication is hindered by the addition of the two inserts into MV genome. Further analysis of MVvac2(DIII-S)N is needed to justify immune response studies in a small animal model using both of the generated recombinant vectors.
ContributorsHarahap, Indira Saridewi (Author) / Reyes del Valle, Jorge (Thesis director) / Hogue, Brenda (Committee member) / Misra, Rajeev (Committee member) / Barrett, The Honors College (Contributor) / T. Denny Sanford School of Social and Family Dynamics (Contributor) / School of Human Evolution and Social Change (Contributor) / School of Life Sciences (Contributor)
Created2014-05
Description
As the student union on Arizona State University’s main Tempe campus, the MU should be a model of building sustainability. After a fire engulfed the MU’s second floor in 2007, the building underwent major renovations and achieved LEED v2.0: Commercial Interiors (LEED CI) Gold certification. Since then, more up-to-date building certification programs have been established, including the Green Globes (GG) green building rating system and a more recent version of LEED that suits the MU’s situation—Existing Buildings: Operations and Maintenance (LEED EB:OM). This paper performs a cost-benefit analysis of these rating systems, specifically looking at 1) national recognition, 2) ease of use, 3) amount of resources needed to invest, 4) length of time of the certification process, 5) certification fees and costs, 6) pre-requisites and system structure of point allotment, and 7) flexibility of the systems. A review of the previous LEED CI certification of the MU addresses 1) solar panel installation and renewable energy achievements, 2) improvement of indoor air quality, 3) application of sustainable construction practices, 4) missed opportunities since renovations were not performed on the entire building, 5) water efficiency scoring, and 6) lack of significant Energy & Atmosphere improvements. A proposal for the university to consider LEED EB:OM certification for the Memorial Union will be presented, analyzing the points already secured by campus-wide programs and policies, credits that are possible with minimal funding, and elaborating on opportunities already planned for completion, including 1) the Power Parasol project, 2) “Zero Waste by 2015” and “Carbon Neutrality” programs and goals, and 3) plans for alternative transportation methods through the Tempe Campus Access Management Plan. In conclusion, my recommendation to pursue LEED EB:OM and achieve Silver level will be presented. With the majority of LEED points already secured and several on the horizon, certifying the MU under LEED EB:OM will set an example and increase the amount of existing buildings on campus to pursue LEED certification. University-wide policies on green cleaning, sustainable purchasing, alternative energy sources, carbon neutrality, and LEED for Multiple Buildings all contribute to simplifying the LEED certification process for ASU buildings campus-wide.
ContributorsHeppner, Mary Catherine (Author) / Brown, Nicholas (Thesis director) / Martin, Thomas (Committee member) / Bogart, Brooke (Committee member) / Barrett, The Honors College (Contributor) / School of Letters and Sciences (Contributor) / T. Denny Sanford School of Social and Family Dynamics (Contributor)
Created2013-05