Matching Items (3)
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- All Subjects: Infectious Diseases
- All Subjects: Risk assessment
- Creators: Jehn, Megan
- Creators: Legutki, Bart
- Member of: Barrett, The Honors College Thesis/Creative Project Collection
Description
The influenza virus, also known as "the flu", is an infectious disease that has constantly affected the health of humanity. There is currently no known cure for Influenza. The Center for Innovations in Medicine at the Biodesign Institute located on campus at Arizona State University has been developing synbodies as a possible Influenza therapeutic. Specifically, at CIM, we have attempted to design these initial synbodies to target the entire Influenza virus and preliminary data leads us to believe that these synbodies target Nucleoprotein (NP). Given that the synbody targets NP, the penetration of cells via synbody should also occur. Then by Western Blot analysis we evaluated for the diminution of NP level in treated cells versus untreated cells. The focus of my honors thesis is to explore how synthetic antibodies can potentially inhibit replication of the Influenza (H1N1) A/Puerto Rico/8/34 strain so that a therapeutic can be developed. A high affinity synbody for Influenza can be utilized to test for inhibition of Influenza as shown by preliminary data. The 5-5-3819 synthetic antibody's internalization in live cells was visualized with Madin-Darby Kidney Cells under a Confocal Microscope. Then by Western Blot analysis we evaluated for the diminution of NP level in treated cells versus untreated cells. Expression of NP over 8 hours time was analyzed via Western Blot Analysis, which showed NP accumulation was retarded in synbody treated cells. The data obtained from my honors thesis and preliminary data provided suggest that the synthetic antibody penetrates live cells and targets NP. The results of my thesis presents valuable information that can be utilized by other researchers so that future experiments can be performed, eventually leading to the creation of a more effective therapeutic for influenza.
ContributorsHayden, Joel James (Author) / Diehnelt, Chris (Thesis director) / Johnston, Stephen (Committee member) / Legutki, Bart (Committee member) / Barrett, The Honors College (Contributor) / Department of Psychology (Contributor) / Department of Chemistry and Biochemistry (Contributor)
Created2014-05
Description
Advancements in both the medical field and public health have substantially minimized the detrimental impact of infectious diseases. Health education and disease prevention remains a vital tool to maintain and propagate this success. In order to determine the relationship between knowledge of disease and reported preventative behavior 180 participants amongst the ASU student population were surveyed about their knowledge and prevention behavior for 10 infectious diseases. Of the 180 participants only 138 were completed surveys and used for analysis. No correlation was found between knowledge or perceived risk and preventative measures within the total sample of 138 respondents, however there was a correlation found within Lyme disease and Giardia exposure to information and prevention. Additionally, a cultural consensus analysis was used to compare the data of 17 US-born and 17 foreign-born participants to analyze patterns of variation and agreement on disease education based on national origins. Cultural consensus analysis showed a strong model of agreement among all participants as well as within the US-born and foreign-born student groups. There was a model of agreement within the questions pertaining to transmission and symptoms. There was not however a model of agreement within treatment questions. The findings suggest that accurate knowledge on infectious diseases may be less impactful on preventative behavior than social expectations.
ContributorsVernon, Samantha (Author) / Maupin, Jonathan (Thesis director) / Jehn, Megan (Committee member) / Barrett, The Honors College (Contributor) / School of Human Evolution and Social Change (Contributor)
Created2018-05
Description
Human health risk assessment is the process by which regulatory agencies estimate the potential for adverse health outcomes as a result of exposure to contaminated food, water, or environmental conditions (US EPA, 2014). However, the risk assessment process typically does not require inputs to be culturally sensitive to the groups facing the potential health outcomes, and the guidelines suggest little emphasis on food security or food sovereignty, concepts which highlight the importance of access to healthy and culturally appropriate foods. This thesis outlines the theoretical concepts of food and environmental justice, framing them in the context of application to land based, rural communities such as Native American groups. This is significant due to the historically disproportionate contamination of Native lands by hazardous waste or other toxins. Three noteworthy case study examples featuring elements of oral exposure pathways to environmental contamination will be outlined and analyzed to articulate how, by incorporating locally-grounded knowledge, a risk assessment could uncover more accurate information, leading to more appropriate and effective mitigation techniques that uphold food and environmental justice principles. Finally, the trade offs between the expansion of local knowledge and the limitations on cultural consumption are discussed, with the conclusion that supports balancing these trade offs through locally grounded, community-driven assessment and mitigation of contamination.
ContributorsDineen, Lillian K (Author) / BurnSilver, Shauna (Thesis director) / Jehn, Megan (Committee member) / Gonzales, Melissa (Committee member) / School of Life Sciences (Contributor) / School of Human Evolution & Social Change (Contributor) / Barrett, The Honors College (Contributor)
Created2019-12