2024-03-29T14:28:39Zhttps://keep.lib.asu.edu/oai/requestoai:keep.lib.asu.edu:node-1573372021-08-27T02:47:01Zoai_pmh:all157337
https://hdl.handle.net/2286/R.I.53762
http://rightsstatements.org/vocab/InC/1.0/
2019
xiii, 117 pages : illustrations (chiefly color)
Doctoral Dissertation
Academic theses
Text
eng
Espiritu, Eduardo
Allen, James P.
Jones, Anne K
Redding, Kevin
Arizona State University
Partial requirement for: Ph.D., Arizona State University, 2019
Includes bibliographical references (pages 96-105)
Field of study: Biochemistry
In my thesis, I characterize multi-nuclear manganese cofactors in modified reaction <br/><br/>centers from the bacterium Rhodobacter sphaeroides. I characterized interactions <br/><br/>between a variety of secondary electron donors and modified reaction centers. In Chapter <br/><br/>1, I provide the research aims, background, and a summary of the chapters in my thesis. <br/><br/>In Chapter 2 and Chapter 3, I present my work with artificial four-helix bundles as <br/><br/>secondary electron donors to modified bacterial reaction centers. In Chapter 2, I <br/><br/>characterize the binding and energetics of the P1 Mn-protein, as a secondary electron <br/><br/>donor to modified reaction centers. In Chapter 3, I present the activity of a suite of four<br/><br/>helix bundles behaving as secondary electron donors to modified reaction centers. In <br/><br/>Chapter 4, I characterize a suite of modified reaction centers designed to bind and oxidize <br/><br/>manganese. I present work that characterizes bound manganese oxides as secondary <br/><br/>electron donors to the oxidized bacteriochlorophyll dimer in modified reaction centers. In <br/><br/>Chapter 5, I present my conclusions with a short description of future work in <br/><br/>characterizing multiple electron transfers from a multi-nuclear manganese cofactor in <br/><br/>modified reaction centers. To conclude, my thesis presents a characterization of a variety <br/><br/>of secondary electron donors to modified reaction centers that establish the feasibility to <br/><br/>characterize multiple turnovers from a multi-nuclear manganese cofactor.
Biochemistry
Photosynthetic reaction centers
Photosynthetic bacteria
Bacteria--Cytochemistry.
Characterization of multi-nuclear manganese-binding bacterial reaction centers from Rhodobacter sphaeroides