2024-03-28T22:49:27Zhttps://keep.lib.asu.edu/oai/requestoai:keep.lib.asu.edu:node-1366612021-08-11T21:09:57Zoai_pmh:all136661
https://hdl.handle.net/2286/R.I.27002
http://rightsstatements.org/vocab/InC/1.0/
2014-12
2016-12-09T09:04:10
50 pages
eng
Lee, Fred
Meldrum, Deirdre R.
Tian, Yanqing
Zhang, Liqiang
Barrett, The Honors College
Chemical Engineering Program
Department of Chemistry and Biochemistry
Text
A series of mitochondria targeting probes was synthesized for the purpose of exploring the feasibility of a mitochondria targeting fluorescent sensor. Of the probes, the probe with a two carbon spacer showed the best co-localization from staining with the established MitoTracker Red® FM, indicating a potential development of the probe into mitochondria targeting sensor. However, cytotoxicity was observed for the probe with a six carbon spacer. Three additional mitochondria targeting fluorescent probes of longer spacer groups were synthesized, but the cytotoxicity was not observed to be as high as that of the probe with a two carbon spacer. The cytotoxicity was characterized to be that of caspase dependent cell death. To screen for a possible effect on apoptosis due to the mitochondrial probe, three fluorescent fusion proteins binding the anti-apoptotic proteins were designed and expressed. Each purified fusion protein was then incubated with the cytotoxic mitochondrial probe, and the mixture was isolated by running an affinity column. The fluorescence analysis of eluted fractions showed preliminary data of possible interaction between the protein and the mitochondrial probe.
BCL-2
Mitochondria
Molecular Cloning
Fluorescent Probes
Organelle-specific Dyes
Biochemistry
Organic Synthesis
Synthesis and Characterization of Mitochondria Targeting Fluorescent Probes