2024-03-29T08:41:00Zhttps://keep.lib.asu.edu/oai/requestoai:keep.lib.asu.edu:node-1302742021-08-16T19:23:30Zoai_pmh:all130274
https://hdl.handle.net/2286/R.I.45082
Reddy, H. K., Yoon, C. H., Aquila, A., Awel, S., Ayyer, K., Barty, A., . . . Xavier, P. L. (2017). Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source. Scientific Data, 4, 170079. doi:10.1038/sdata.2017.79
10.1038/sdata.2017.79
2052-4463
http://rightsstatements.org/vocab/InC/1.0/
http://creativecommons.org/licenses/by/4.0
2017-06-27
9 pages
eng
Reddy, Hemanth K. N.
Yoon, Chun Hong
Aquila, Andrew
Awel, Salah
Ayyer, Kartik
Barty, Anton
Berntsen, Peter
Bielecki, Johan
Bobkov, Sergey
Bucher, Maximilian
Carini, Gabriella A.
Carron, Sebastian
Chapman, Henry
Daurer, Benedikt
DeMirci, Hasan
Ekeberg, Tomas
Fromme, Petra
Hajdu, Janos
Hanke, Max Felix
Hart, Philip
Hogue, Brenda
Hasseinizadeh, Ahmad
Kim, Yoonhee
Kirian, Richard
Kurta, Ruslan P.
Larsson, Daniel S. D.
Loh, N. Duane
Maia, Filipe R. N. C.
Mancuso, Adrian P.
Muhlig, Kerstin
Munke, Anna
Nam, Daewoong
Nettelblad, Carl
Ourmazd, Abbas
Rose, Max
Schwander, Peter
Seibert, Marvin
Sellberg, Jonas A.
Song, Changyong
Spence, John
Svenda, Martin
van der Schot, Gijs
Vartanyants, Ivan A.
Williams, Garth J.
Xavier, P. Lourdu
ASU Biodesign Center Immunotherapy, Vaccines and Virotherapy
Biodesign Institute
Applied Structural Discovery
College of Liberal Arts and Sciences
School of Molecular Sciences
School of Life Sciences
Department of Physics
The final version of this article, as published in Scientific Data, can be viewed online at: http://www.nature.com/articles/sdata201779
Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without the need for crystallization. In an effort to further develop the technique, we present a dataset of coherent soft X-ray diffraction images of Coliphage PR772 virus, collected at the Atomic Molecular Optics (AMO) beamline with pnCCD detectors in the LAMP instrument at the Linac Coherent Light Source. The diameter of PR772 ranges from 65–70 nm, which is considerably smaller than the previously reported ~600 nm diameter Mimivirus. This reflects continued progress in XFEL-based single-particle imaging towards the single molecular imaging regime. The data set contains significantly more single particle hits than collected in previous experiments, enabling the development of improved statistical analysis, reconstruction algorithms, and quantitative metrics to determine resolution and self-consistency.
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Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source