2024-03-19T08:29:32Zhttps://keep.lib.asu.edu/oai/requestoai:keep.lib.asu.edu:node-1280102021-10-26T21:23:48Zoai_pmh:all128010
https://hdl.handle.net/2286/R.I.45301
<p>Soni, A., O’Sullivan, L., Quick, L. N., Ott, C. M., Nickerson, C. A., & Wilson, J. W. (2014). Conservation of the Low-shear Modeled Microgravity Response in Enterobacteriaceae and Analysis of the trp Genes in this Response. The Open Microbiology Journal, 8(1), 51-58. doi:10.2174/1874285801408010051</p>
10.2174/1874285801408010051
1874-2858
2014
8 pages
eng
Soni, Anjali
O'Sullivan, Laura
Quick, Laura N.
Ott, C. Mark
Nickerson, Cheryl
Wilson, James W.
ASU Biodesign Center Immunotherapy, Vaccines and Virotherapy
Biodesign Institute
The final version of this article, as published in he Open Microbiology Journal, can be viewed online at: https://benthamopen.com/ABSTRACT/TOMICROJ-8-51
<p>Low fluid shear force, including that encountered in microgravity models, induces bacterial responses, but the range of bacteria capable of responding to this signal remains poorly characterized. We systematically analyzed a range of Gram negative Enterobacteriaceae for conservation of the low-shear modeled microgravity (LSMMG) response using phenotypic assays, qPCR, and targeted mutations. Our results indicate LSMMG response conservation across Enterobacteriacae with potential variance in up- or down-regulation of a given response depending on genus. Based on the data, we analyzed the role of the trp operon genes and the TrpR regulator in the LSMMG response using targeted mutations in these genes in S. Typhimurium and E. coli. We found no alteration of the LSMMG response compared to WT in these mutant strains under the conditions tested here. To our knowledge, this study is first-of-kind for Citrobacter, Enterobacter, and Serratia, presents novel data for Escherichia, and provides the first analysis of trp genes in LSMMG responses. This impacts our understanding of how LSMMG affects bacteria and our ability to modify bacteria with this condition in the future.</p>
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Conservation of the Low-Shear Modeled Microgravity Response in Enterobacteriaceae and Analysis of the TRP Genes in This Response