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oai:keep.lib.asu.edu:node-2008122025-06-02T23:54:19Zoai_pmh:alloai_pmh:repo_items

200812 https://hdl.handle.net/2286/R.2.N.200812 http://rightsstatements.org/vocab/InC/1.0/ http://creativecommons.org/licenses/by-nc-sa/4.0 2025-05 11 pages Patel, Anjali Clark, Heather Ma, Kristine Barrett, The Honors College School of Life Sciences Sanford School of Social and Family Dynamics Phosphorodiamidate Morpholino Oligomers (PMOs) are synthetic nucleic acid analogs designed for enhanced stability and resistance to enzymatic degradation compared to DNA and RNA. In this study, we examine the stability of different PMO-hybrid duplexes, specifically PMO:PMO, PMO:DNA, and PMO:LNA, using gel electrophoresis to determine their relative half-lives when put under biological conditions. Each duplex is then conjugated with gadolinium-based contrast agent Gd-DOTA to test their stability using gel electrophoresis once again, confirming the duplex’s half life is relatively unchanged compared to its unconjugated duplex when exposed to enzymatic degradation conditions. This study aims to bridge the gap between PMO-based therapeutics and molecular imaging by introducing a stable scaffold method and evaluating how conjugation impacts stability in biological settings. PMO Testing the Stability of Different PMO-Hybrid Duplexes