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oai:keep.lib.asu.edu:node-1645042024-12-19T19:04:43Zoai_pmh:alloai_pmh:repo_items

164504 https://hdl.handle.net/2286/R.2.N.164504 http://rightsstatements.org/vocab/InC/1.0/ http://creativecommons.org/licenses/by-nc-sa/4.0 2022-05 2023-04-15T05:00:00 Van Stone, Alicia Anderson, Karen Buetow, Kenneth Knappenberger, Mark Barrett, The Honors College College of Integrative Sciences and Arts School of Life Sciences Text <p>The Human Leukocyte Antigen (HLA) is a protein on the surface of cells that is a large component of the adaptive immune response as it helps recognize foreign pathogenic material. We wonder if a set of primers designed for each HLA type could be used to amplify a wide spectrum of HLA to improve sequencing of HLA to improve HLA-typing access. We propose the use of an HLA allele panel to determine the pulldown capacity of the primers followed by MinION sequencing and also offer a multiplexing design for running 96 patients at once. Our results show that primers can capture Class I HLA alleles and typing was successful with an average alignment accuracy of 91.7%. In conclusion this method for HLA capture could be utilized for HLA-typing with material costs of under $3.00 per sample within 3 days.</p> Human Leukocyte Antigen MinION Nanopore Sequencing Point of Care Major Histocompatibility Complex Efficient Methods of Human Leukocyte Antigen Capture in a Point of Care Setting