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154114 https://hdl.handle.net/2286/R.I.36376 http://rightsstatements.org/vocab/InC/1.0/ All Rights Reserved 2015 iv, 123 pages Masters Thesis Academic theses Text eng Bartlett, Zane Maienschein, Jane Ellison, Karin Hurlbut, James Arizona State University Partial requirement for: M.S., Arizona State University, 2015 Includes bibliographical references Field of study: Biology Researchers in the twentieth and twenty-first centuries identify the study of the intrinsic and external factors that influence human aging as senescence. A commonly held belief in the year 2015 is that at least some kinds of cells can replicate over long periods or even indefinitely, thereby meaning the cell does not undergo senescence (also known as replicative senescence) and is considered immortal. This study aims to provide information to answer the following question: While some scientists claim they can indefinitely culture a stem cell line in vitro, what are the consequences of those culturing practices? An analysis of a cluster of articles from the Embryo Project Encyclopedia provides information to suggest possible solutions to some potential problems in cell culturing, recognition of benefits for existing or historical culturing practices, and identification of gaps in scientific knowledge that warrant further research. Recent research suggests that hESCs, and immortalized cell lines in general, do not escape the effects of senescence. While there exists a constant change in the practices of cell culturing, a large portion of scientists still rely on practices established before modern senescence research: research that seems to suggest that cultured hESCs, among other immortal cell lines, are not truly immortal. Biology History Cellular Biology Aging human embryonic stem cells immortal cell lines immortal cells senescence Stem Cells Stem Cells Aging Cell culture Cytology A history of cellular senescence and its relation to stem cells in the twentieth and twenty-first centuries