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136483 https://hdl.handle.net/2286/R.I.28810 http://rightsstatements.org/vocab/InC/1.0/ 2015-05 28 pages eng Tao, Kevin Huang Sierks, Michael Williams, Stephanie Barrett, The Honors College Chemical Engineering Program Text The research objective is to maintain the A4 nanobody stability during dialysis. Various dialysis buffers were tested and compared, including PBS with varying amounts of the detergent, Tween: low, high, none. Furthermore, PBS, Tris, and HEPES, were tested and compared. PBS without Tween was the worst for preserving A4 stability. PBS was determined to be a better dialysis buffer than Tris or HEPES. To find the optimum buffer, other buffers will be tested and compared with PBS; methods such as gravity filtration and lyophilization will be considered as alternatives to dialysis. Dialysis Alzheimer's Disease Antibodies Optimizing Dialysis Conditions for A4 Stability