In this study, the stability of two protein homo-oligomers, the β clamp (homodimer) from E. coli and the Proliferation Cell Nuclear Antigen (PCNA) from the yeast cell, were characterized. These clamps open through one interface by another protein called clamp loader, which helps it to encircle the DNA template strand. The β clamp protein binds with DNA polymerase and helps it to slide through the template strand and prevents its dissociation from the template strand.
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- Doctoral Dissertation Biochemistry 2020