With the growth of autonomous vehicles’ prevalence, it is important to understand the relationship between autonomous vehicles and the other drivers around them. More specifically, how does one’s knowledge about autonomous vehicles (AV) affect positive and negative affect towards driving in their presence? Furthermore, how does trust of autonomous vehicles correlate with those emotions? These questions were addressed by conducting a survey to measure participant’s positive affect, negative affect, and trust when driving in the presence of autonomous vehicles. Participants’ were issued a pretest measuring existing knowledge of autonomous vehicles, followed by measures of affect and trust. After completing this pre-test portion of the study, participants were given information about how autonomous vehicles work, and were then presented with a posttest identical to the pretest. The educational intervention had no effect on positive or negative affect, though there was a positive relationship between positive affect and trust and a negative relationship between negative affect and trust. These findings will be used to inform future research endeavors researching trust and autonomous vehicles using a test bed developed at Arizona State University. This test bed allows for researchers to examine the behavior of multiple participants at the same time and include autonomous vehicles in studies.