Highly Multiplexed Single Cell in situ Protein Analysis with Cleavable Fluorescent Probes
An azide-based chemical cleavable linker has been introduced to design and synthesis novel fluorescent probes. These probes allow cyclic immunofluorescence staining which leads to the feasibility of highly multiplexed single cell in situ protein profiling. These highly multiplexed imaging-based platforms have the potential to quantify more than 100 protein targets in cultured cells and more than 50 protein targets in single cells in tissues.
This approach has been successfully applied in formalin-fixed paraffin-embedded (FFPE) brain tissues. Multiplexed protein expression level results reveal neuronal heterogeneity in the human hippocampus.]]>autLiao, RenjiethsGuo, JiadgcBorges, ChaddgcLiu, YanpblArizona State UniversityengPartial requirement for: Ph.D., Arizona State University, 2019Includes bibliographical references (pages 105-120)Field of study: Chemistryby Renjie Liaohttps://hdl.handle.net/2286/R.I.5360500Doctoral DissertationAcademic thesesix, 125 pages : illustrations (some color)115579412631630032421157181systemIn Copyright2019TextChemistryFFPE tissuesFluorescent ProbesImmunofluorescenceproteomicsSingle Cellstyramide signal amplificationFluorescence in situ hybridizationFluorescent ProbesSingle cell proteins--Analysis.Single cell proteins