Early detection and treatment of breast cancer by random peptide array in neuN transgenic mouse model
In this dissertation, I propose a scenario for using immunosignature technology to detect breast cancer early and to implement an early treatment strategy by using the PD-L1 immune checkpoint inhibitor. I develop a methodology to describe the early diagnosis and treatment of breast cancer in a FVB/N neuN breast cancer mouse model. By comparing FVB/N neuN transgenic mice and age-matched wild type controls, I have found and validated specific immunosignatures at multiple time points before tumors are palpable. Immunosignatures change along with tumor development. Using a late-stage immunosignature to predict early samples, or vice versa, cannot achieve high prediction performance. By using the immunosignature of early breast cancer, I show that at the time of diagnosis, early treatment with the checkpoint blockade, anti-PD-L1, inhibits tumor growth in FVB/N neuN transgenic mouse model. The mRNA analysis of the PD-L1 level in mice mammary glands suggests that it is more effective to have treatment early.
Novel discoveries are changing understanding of breast cancer and improving strategies in clinical treatment. Researchers and healthcare professionals are actively working in the early diagnosis and early treatment fields. This dissertation provides a step along the road for better diagnosis and treatment of breast cancer.]]>autDuan, HuthsJohnston, Stephen AlbertdgcHartwell, Leland HarrisondgcDinu, ValentindgcChang, YungpblArizona State UniversityengPartial requirement for: Ph. D., Arizona State University, 2015Includes bibliographical references (pages 93-117)Field of study: Biological designby Hu Duanhttps://hdl.handle.net/2286/R.I.3491600Doctoral DissertationAcademic thesesxv, 152 pages : illustrations (some color)114398306091630348022153975adminIn CopyrightAll Rights Reserved2015TextBiomedical EngineeringAnitbodybiomarkerBreast CancerEarly DiagnosisMicroarrayMouse ModelBiochemical markersTransgenic miceProtein microarraysBreast--Cancer--Diagnosis.Breast--Cancer--Treatment.