Description
Transgene expression in mammalian cells has been shown to meet resistance in the form of silencing due to chromatin buildup within the cell. Interactions of proteins with chromatin modulate gene expression profiles. Synthetic Polycomb transcription factor (PcTF) variants have the potential to reactivate these silence transgenes as shown in Haynes & Silver 2011. PcTF variants have been constructed via TypeIIS assembly to further investigate this ability to reactive transgenes.
Copyright Statement
Download count: 0
Details
Contributors
- Gardner, Cameron Lee (Author)
- Haynes, Karmella (Thesis director)
- Stabenfeldt, Sarah (Committee member)
- Barrett, The Honors College (Contributor)
- Department of Finance (Contributor)
- Harrington Bioengineering Program (Contributor)
Date Created
- 2015-05
Resource Type
Collections this item is in