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  1. KEEP
  2. Theses and Dissertations
  3. Barrett, The Honors College Thesis/Creative Project Collection
  4. Using Natural Diversity of Quorum Sensing to Expand the Synthetic Biology Toolbox
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Using Natural Diversity of Quorum Sensing to Expand the Synthetic Biology Toolbox

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Description

Currently in synthetic biology only the Las, Lux, and Rhl quorum sensing pathways have been adapted for broad engineering use. Quorum sensing allows a means of cell to cell communication in which a designated sender cell produces quorum sensing molecules that modify gene expression of a designated receiver cell. While useful, these three quorum sensing pathways exhibit a nontrivial level of crosstalk, hindering robust engineering and leading to unexpected effects in a given design. To address the lack of orthogonality among these three quorum sensing pathways, previous scientists have attempted to perform directed evolution on components of the quorum sensing pathway. While a powerful tool, directed evolution is limited by the subspace that is defined by the protein. For this reason, we take an evolutionary biology approach to identify new orthogonal quorum sensing networks and test these networks for cross-talk with currently-used networks. By charting characteristics of acyl homoserine lactone (AHL) molecules used across quorum sensing pathways in nature, we have identified favorable candidate pathways likely to display orthogonality. These include Aub, Bja, Bra, Cer, Esa, Las, Lux, Rhl, Rpa, and Sin, which we have begun constructing and testing. Our synthetic circuits express GFP in response to a quorum sensing molecule, allowing quantitative measurement of orthogonality between pairs. By determining orthogonal quorum sensing pairs, we hope to identify and adapt novel quorum sensing pathways for robust use in higher-order genetic circuits.

Date Created
2015-05
Contributors
  • Muller, Ryan (Author)
  • Haynes, Karmella (Thesis director)
  • Wang, Xiao (Committee member)
  • Barrett, The Honors College (Contributor)
  • School of Mathematical and Statistical Sciences (Contributor)
  • Department of Chemistry and Biochemistry (Contributor)
  • School of Life Sciences (Contributor)
Topical Subject
  • Synthetic Biology
  • Quorum Sensing
  • Orthogonality
Resource Type
Text
Extent
26 pages
Language
eng
Copyright Statement
In Copyright
Primary Member of
Barrett, The Honors College Thesis/Creative Project Collection
Series
Academic Year 2014-2015
Handle
https://hdl.handle.net/2286/R.I.29349
Embargo Release Date
Wed, 05/03/2017 - 03:55
Level of coding
minimal
Cataloging Standards
asu1
System Created
  • 2017-10-30 02:50:57
System Modified
  • 2021-08-11 04:09:57
  •     
  • 1 year 5 months ago
Additional Formats
  • OAI Dublin Core
  • MODS XML

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