Effects of Environmental Conditions on Pyocyanin Production in Pseudomonas aeruginosa Through a liquid-liquid extraction procedure, pyocyanin was quantified in cultures that were incubated at 30°C, 37°C, and 40°C and in the presence of Staphylococcus aureus spent media. In addition, culturing methods for the measurement of pyocyanin under hypoxic conditions were analyzed. I hypothesized that environmental conditions such as temperature, co-infection with S. aureus, and oxygen depletion would influence pyocyanin production. It was found that overall, 30°C incubation produced statistically significant decrease in pyocyanin production compared with incubation at 37°C. These findings will help to determine how phenotypes are affected by conditions in the CF lung. In addition, these conclusions will help direct metabolic analysis and to identify volatile biomarkers of pyocyanin production for future use in breath-based diagnostics of CF lung infections.]]>cauWitzel, LeacauBean, Heather D.thsBean, Heather D.dgcMisra, RajeevdgcHaydel, ShelleyctbSchool of Life SciencesctbBarrett, The Honors Collegeenghttps://hdl.handle.net/2286/R.I.4269141 pages115093930581628716197134816lcwitzelIn Copyright2016-12TextCystic FibrosisP. aeruginosaPyocyanin