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The impetus for discovery and evaluation of protein biomarkers has been accelerated by recent development of advanced technologies for rapid and broad proteome analyses. Mass spectrometry (MS)-based protein assays hold

The impetus for discovery and evaluation of protein biomarkers has been accelerated by recent development of advanced technologies for rapid and broad proteome analyses. Mass spectrometry (MS)-based protein assays hold great potential for in vitro biomarker studies. Described here is the development of a multiplex mass spectrometric immunoassay (MSIA) for quantification of apolipoprotein C-I (apoC-I), apolipoprotein C-II (apoC-II), apolipoprotein C-III (apoC-III) and their proteoforms. The multiplex MSIA assay was fast (∼40 min) and high-throughput (96 samples at a time).

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    Date Created
    • 2015-06-15
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  • Text
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    Identifier
    • Digital object identifier: 10.1016/j.ymeth.2015.02.020
    • Identifier Type
      International standard serial number
      Identifier Value
      1046-2023
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    Trenchevska, Olgica, Schaab, Matthew R., Nelson, Randall W., & Nedelkov, Dobrin (2015). Development of multiplex mass spectrometric immunoassay for detection and quantification of apolipoproteins C-I, C-II, C-III and their proteoforms. METHODS, 81, 86-92. http://dx.doi.org/10.1016/j.ymeth.2015.02.020

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