The low pH of the stomach serves as a barrier to ingested microbes and must be overcome or bypassed when delivering live bacteria for vaccine or probiotic applications. Typically, the impact of stomach acidity on bacterial survival is evaluated in vitro, as there are no small animal models to evaluate these effects in vivo. To better understand the effect of this low pH barrier to live attenuated Salmonella vaccines, which are often very sensitive to low pH, we investigated the value of the histamine mouse model for this application.
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- Digital object identifier: 10.1371/journal.pone.0087411
- Identifier TypeInternational standard serial numberIdentifier Value1045-3830
- Identifier TypeInternational standard serial numberIdentifier Value1939-1560
- The article is published at http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0087411, opens in a new window
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Brenneman, K. E., Willingham, C., Kilbourne, J. A., 3Rd, R. C., & Roland, K. L. (2014). A Low Gastric pH Mouse Model to Evaluate Live Attenuated Bacterial Vaccines. PLoS ONE, 9(1). doi:10.1371/journal.pone.0087411