Leucine-responsive regulatory protein (Lrp) is known to be an indirect activator of type 1 fimbriae synthesis in Salmonella enterica serovar Typhimurium via direct regulation of FimZ, a direct positive regulator for type 1 fimbriae production. Using RT-PCR, we have shown previously that fimA transcription is dramatically impaired in both lrp-deletion (Δlrp) and constitutive-lrp expression (lrp[superscript C]) mutant strains. In this work, we used chromosomal P[subscript fimA]-lacZ fusions and yeast agglutination assays to confirm and extend our previous results.
Download count: 0
- Digital object identifier: 10.1371/journal.pone.0026896
- Identifier TypeInternational standard serial numberIdentifier Value1045-3830
- Identifier TypeInternational standard serial numberIdentifier Value1939-1560
- The article is published at http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0026896, opens in a new window
Citation and reuse
Cite this item
This is a suggested citation. Consult the appropriate style guide for specific citation guidelines.
Baek, C., Kang, H., Roland, K. L., & Curtiss, R. (2011). Lrp Acts as Both a Positive and Negative Regulator for Type 1 Fimbriae Production in Salmonella enterica Serovar Typhimurium. PLoS ONE, 6(10). doi:10.1371/journal.pone.0026896