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  4. Immunogenic Subviral Particles Displaying Domain III of Dengue 2 Envelope Protein Vectored by Measles Virus
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Immunogenic Subviral Particles Displaying Domain III of Dengue 2 Envelope Protein Vectored by Measles Virus

Full metadata

Title
Immunogenic Subviral Particles Displaying Domain III of Dengue 2 Envelope Protein Vectored by Measles Virus
Description

Vaccines against dengue virus (DV) are commercially nonexistent. A subunit vaccination strategy may be of value, especially if a safe viral vector acts as biologically active adjuvant. In this paper, we focus on an immunoglobulin-like, independently folded domain III (DIII) from DV 2 envelope protein (E), which contains epitopes that elicits highly specific neutralizing antibodies. We modified the hepatitis B small surface antigen (HBsAg, S) in order to display DV 2 DIII on a virus-like particle (VLP), thus generating the hybrid antigen DIII-S. Two varieties of measles virus (MV) vectors were developed to express DIII-S. The first expresses the hybrid antigen from an additional transcription unit (ATU) and the second additionally expresses HBsAg from a separate ATU. We found that this second MV vectoring the hybrid VLPs displaying DIII-S on an unmodified HBsAg scaffold were immunogenic in MV-susceptible mice (HuCD46Ge-IFNar[superscript ko]), eliciting robust neutralizing responses (averages) against MV (1:1280 NT90), hepatitis B virus (787 mIU/mL), and DV2 (1:160 NT50) in all of the tested animals. Conversely, the MV vector expressing only DIII-S induced immunity against MV alone. In summary, DV2 neutralizing responses can be generated by displaying E DIII on a scaffold of HBsAg-based VLPs, vectored by MV.

Date Created
2015-07-03
Contributors
  • Harahap, Indira (Author)
  • Ceballos-Olvera, Ivonne (Author)
  • Reyes del Valle, Jorge (Author)
  • College of Liberal Arts and Sciences (Contributor)
Resource Type
Text
Extent
16 pages
Language
eng
Copyright Statement
In Copyright
Reuse Permissions
Attribution
Primary Member of
ASU Scholarship Showcase
Peer-reviewed
No
Open Access
No
Series
VACCINES
Handle
https://hdl.handle.net/2286/R.I.43120
Preferred Citation

Harahap-Carrillo, I., Ceballos-Olvera, I., & Valle, J. (2015). Immunogenic Subviral Particles Displaying Domain III of Dengue 2 Envelope Protein Vectored by Measles Virus. Vaccines, 3(3), 503-518. doi:10.3390/vaccines3030503

Level of coding
minimal
Cataloging Standards
asu1
System Created
  • 2017-04-19 04:02:49
System Modified
  • 2021-12-02 03:33:52
  •     
  • 1 year 11 months ago
Additional Formats
  • OAI Dublin Core
  • MODS XML

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