Solid-state NMR and molecular dynamics (MD) simulations are presented to help elucidate the molecular secondary structure of poly(Gly-Gly-X), which is one of the most common structural repetitive motifs found in orb-weaving dragline spider silk proteins. The combination of NMR and computational experiments provides insight into the molecular secondary structure of poly(Gly-Gly-X) segments and provides further support that these regions are disordered and primarily non-β-sheet. Furthermore, the combination of NMR and MD simulations illustrate the possibility for several secondary structural elements in the poly(Gly-Gly-X) regions of dragline silks, including β-turns, 3[subscript 10]-helicies, and coil structures with a negligible population of α-helix observed.
Gray, G., Vaart, A. V., Guo, C., Jones, J., Onofrei, D., Cherry, B., . . . Holland, G. (2016). Secondary Structure Adopted by the Gly-Gly-X Repetitive Regions of Dragline Spider Silk. International Journal of Molecular Sciences, 17(12), 2023. doi:10.3390/ijms17122023
- 2017-05-24 06:24:56
- 2021-06-17 05:40:09
- 3 months ago