Mechanical interactions between cells and their microenvironment dictate cell phenotype and behavior, calling for cell mechanics measurements in three-dimensional (3D) extracellular matrices (ECM). Here we describe a novel technique for quantitative mechanical characterization of soft, heterogeneous samples in 3D. The technique is based on the integration of atomic force microscopy (AFM) based deep indentation, confocal fluorescence microscopy, finite element (FE) simulations and analytical modeling.
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- Staunton, Jack (Contributor)
- Doss, Bryant (Contributor)
- Lindsay, Stuart (Contributor)
- Ros, Robert (Contributor)
- College of Liberal Arts and Sciences (Contributor)
- 2016-01-27
- Digital object identifier: 10.1038/srep19686
- Identifier TypeInternational standard serial numberIdentifier Value2045-2322
- The final version of this article, as published in Scientific Reports, can be viewed online at: https://www.nature.com/articles/srep19686, opens in a new window
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Staunton, J. R., Doss, B. L., Lindsay, S., & Ros, R. (2016). Correlating confocal microscopy and atomic force indentation reveals metastatic cancer cells stiffen during invasion into collagen I matrices. Scientific Reports, 6(1). doi:10.1038/srep19686