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Mechanical interactions between cells and their microenvironment dictate cell phenotype and behavior, calling for cell mechanics measurements in three-dimensional (3D) extracellular matrices (ECM). Here we describe a novel technique for

Mechanical interactions between cells and their microenvironment dictate cell phenotype and behavior, calling for cell mechanics measurements in three-dimensional (3D) extracellular matrices (ECM). Here we describe a novel technique for quantitative mechanical characterization of soft, heterogeneous samples in 3D. The technique is based on the integration of atomic force microscopy (AFM) based deep indentation, confocal fluorescence microscopy, finite element (FE) simulations and analytical modeling.

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    Date Created
    • 2016-01-27
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    Identifier
    • Digital object identifier: 10.1038/srep19686
    • Identifier Type
      International standard serial number
      Identifier Value
      2045-2322
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    Staunton, J. R., Doss, B. L., Lindsay, S., & Ros, R. (2016). Correlating confocal microscopy and atomic force indentation reveals metastatic cancer cells stiffen during invasion into collagen I matrices. Scientific Reports, 6(1). doi:10.1038/srep19686

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